Restriction map for
pFN21AE4080
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstYI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco52I EcoNI EcoO109I EcoT22I
FauI FseI FspI HapII HgaI
Hin1I HpaI HpaII KasI KpnI
Ksp632I KspI MaeI MfeI MflI
MluI MluNI MroI MscI MspI
MunI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TseI XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AfaI 54 GTAC GT^AC
AlwNI 740 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 898 CCTCAGG CC^T[ACGT]AGG
Aor51HI 439 AGCGCT AGC^GCT
Asp700I 451 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 256 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 522 GGACC G^G[AT]CC
BanI 990 GGTGCC G^G[CT][GA]CC
BclI 720 TGATCA T^GATCA
BsaAI 1019 TACGTG [CT]AC^GT[GA]
BsaBI 126 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 557 CCATGG C^C[ACGT][ACGT]GG
BsrDI 1150 GCAATG GCAATG(2/0)
BstAPI 579 GCAACATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 103 GGTTACC G^GT[ACGT]ACC
BstPI 103 GGTTACC G^GT[ACGT]ACC
Bsu36I 898 CCTCAGG CC^T[ACGT]AGG
Cfr13I 522 GGACC G^G[ACGT]CC
DraIII 1016 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 557 CCATGG C^C[GA][CT]GG
Eco47III 439 AGCGCT AGC^GCT
Eco81I 898 CCTCAGG CC^T[ACGT]AGG
EcoO65I 103 GGTTACC G^GT[ACGT]ACC
EcoRI 1005 GAATTC G^AATTC
EcoRV 619 GATATC GAT^ATC
EcoT14I 557 CCATGG C^C[AT][AT]GG
FbaI 720 TGATCA T^GATCA
Fnu4HI 407 GCCGC GC^[ACGT]GC
HaeII 439 AGCGCT [GA]GCGC^[CT]
HaeIII 1169 GGCC GG^CC
HincII 794 GTCAAC GT[CT]^[GA]AC
HindII 794 GTCAAC GT[CT]^[GA]AC
HindIII 384 AAGCTT A^AGCTT
ItaI 407 GCCGC GC^[ACGT]GC
MaeII 1020 ACGT A^CGT
MamI 126 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 800 CAGCGG C[AC]G^C[GT]G
NcoI 557 CCATGG C^CATGG
NdeI 583 CATATG CA^TATG
NspI 222 GCATGC [GA]CATG^[CT]
PflFI 256 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1209 GAGTC GAGTC(4/5)
RsaI 54 GTAC GT^AC
SacI 419 GAGCTC GAGCT^C
Sau96I 522 GGACC G^G[ACGT]CC
ScaI 53 AGTACT AGT^ACT
SmlI 1122 CTTGAG C^T[CT][GA]AG
SphI 222 GCATGC GCATG^C
StyI 557 CCATGG C^C[AT][AT]GG
TfiI 742 GATTC G^A[AT]TC
Tth111I 256 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1029 CCAGTCTCAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 451 GAAAAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccII 281 CGCG CG^CG
1205 CGCG
AlwI 961 GATCC GGATC(4/5)
1155 GGATC
AspHI 419 GAGCTC G[AT]GC[AT]^C
1022 GTGCTC
BanII 419 GAGCTC G[GA]GC[CT]^C
1125 GAGCCC
BbsI 1259 GAAGAC GAAGAC(2/6)
331 GTCTTC
BpuAI 1259 GAAGAC GAAGAC(2/6)
331 GTCTTC
BsiHKAI 419 GAGCTC G[AT]GC[AT]^C
1022 GTGCTC
BsmAI 375 GTCTC GTCTC(1/5)
1032 GTCTC
BssKI 100 CCTGG ^CC[ACGT]GG
624 CCAGG
BstNI 100 CCTGG CC^[AT]GG
624 CCAGG
BstUI 281 CGCG CG^CG
1205 CGCG
EcoRII 100 CCTGG ^CC[AT]GG
624 CCAGG
HinfI 742 GATTC G^A[ACGT]TC
1209 GAGTC
MboII 1259 GAAGA GAAGA(8/7)
332 TCTTC
MslI 163 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
555 CACCATGGTG
MvaI 100 CCTGG CC^[AT]GG
624 CCAGG
MvnI 281 CGCG CG^CG
1205 CGCG
PflMI 665 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCATCATCTGG
ScrFI 100 CCTGG CC^[ACGT]GG
624 CCAGG
SfaNI 9 GATGC GCATC(5/9)
1147 GATGC
TaqI 423 TCGA T^CGA
1236 TCGA
TthHB8I 423 TCGA T^CGA
1236 TCGA
Van91I 665 CCATCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCATCATCTGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcsI AluI ApoI BmyI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrI BstF5I Cac8I CfoI DdeI
DpnII FokI HhaI HinP1I HphI
MaeIII MboI MnlI MseI MwoI
NdeII NlaIII NlaIV Sau3AI Tru9I
Tsp45I Tsp509I TspRI