Restriction map for
pFN21AE4179
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbsI BcgI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsiWI
BsmBI BsmI BspDI BspHI BspLU11I
BsrDI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI Eam1105I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MflI MluI MluNI MscI
MseI MunI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
StuI SwaI Tru9I Tth111I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 824 TCCGGA T^CCGGA
AcsI 115 GAATTC [GA]^AATT[CT]
AlwI 207 GATCC GGATC(4/5)
AocI 1501 CCTGAGG CC^T[ACGT]AGG
ApoI 115 GAATTC [GA]^AATT[CT]
AviII 1852 TGCGCA TGC^GCA
BbeI 1639 GGCGCC GGCGC^C
BbrPI 217 CACGTG CAC^GTG
BbvCI 534 GCTGAGG CCTCAGC(-5/-2)
BsaAI 217 CACGTG [CT]AC^GT[GA]
BsaI 100 GAGACC GGTCTC(1/5)
BsaWI 824 TCCGGA [AT]^CCGG[AT]
BseAI 824 TCCGGA T^CCGGA
BspEI 824 TCCGGA T^CCGGA
BssSI 724 CTCGTG CACGAG(-5/-1)
Bsu36I 1501 CCTGAGG CC^T[ACGT]AGG
DraIII 1075 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1483 GACTTATACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1501 CCTGAGG CC^T[ACGT]AGG
EcoRI 115 GAATTC G^AATTC
FspI 1852 TGCGCA TGC^GCA
KasI 1639 GGCGCC G^GCGCC
MroI 824 TCCGGA T^CCGGA
MslI 1560 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 232 GCCGGC GCC^GGC
NarI 1639 GGCGCC GG^CGCC
NgoMIV 232 GCCGGC G^CCGGC
NotI 1662 GCGGCCGC GC^GGCCGC
PflMI 999 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
PshAI 1339 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 324 CCTGCAGG CCTGCA^GG
SfoI 1639 GGCGCC GGC^GCC
SmlI 1735 CTCAAG C^T[CT][GA]AG
Sse8387I 324 CCTGCAGG CCTGCA^GG
TfiI 190 GAATC G^A[AT]TC
Tsp509I 116 AATT ^AATT
Van91I 999 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1058 CCACACCACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 780 GACGCC G[GA]^CG[CT]C
1639 GGCGCC
Alw44I 1083 GTGCAC G^TGCAC
1183 GTGCAC
Aor51HI 452 AGCGCT AGC^GCT
1625 AGCGCT
ApaLI 1083 GTGCAC G^TGCAC
1183 GTGCAC
BfaI 914 CTAG C^TAG
1915 CTAG
BglI 403 GCCAACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1124 GCCCTTCCGGC
BsaHI 780 GACGCC G[GA]^CG[CT]C
1639 GGCGCC
BsgI 214 CTGCAC GTGCAG(16/14)
1387 CTGCAC
BsmAI 100 GAGAC GTCTC(1/5)
1711 GAGAC
BsrBI 796 CCGCTC CCGCTC(-3/-3)
1839 GAGCGG
BsrFI 49 ACCGGC [GA]^CCGG[CT]
232 GCCGGC
BstAPI 240 GCAGCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
474 GCAGGAGCTGC
Cfr10I 49 ACCGGC [GA]^CCGG[CT]
232 GCCGGC
DpnII 207 GATC ^GATC
1620 GATC
EagI 446 CGGCCG C^GGCCG
1663 CGGCCG
EclXI 446 CGGCCG C^GGCCG
1663 CGGCCG
Eco47III 452 AGCGCT AGC^GCT
1625 AGCGCT
Eco52I 446 CGGCCG C^GGCCG
1663 CGGCCG
EcoT14I 284 CCATGG C^C[AT][AT]GG
1532 CCATGG
Hin1I 780 GACGCC G[GA]^CG[CT]C
1639 GGCGCC
MaeI 914 CTAG C^TAG
1915 CTAG
MboI 207 GATC ^GATC
1620 GATC
NcoI 284 CCATGG C^CATGG
1532 CCATGG
NdeII 207 GATC ^GATC
1620 GATC
PvuII 373 CAGCTG CAG^CTG
1864 CAGCTG
SacI 517 GAGCTC GAGCT^C
991 GAGCTC
Sau3AI 207 GATC ^GATC
1620 GATC
SmaI 194 CCCGGG CCC^GGG
737 CCCGGG
StyI 284 CCATGG C^C[AT][AT]GG
1532 CCATGG
XmaCI 194 CCCGGG C^CCGGG
737 CCCGGG
XmaI 194 CCCGGG C^CCGGG
737 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EaeI
EarI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I KspI MaeII MaeIII
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
PleI PstI RsaI SacII Sau96I
ScrFI SfaNI SfcI SspBI TaqI
TseI Tsp45I TspRI TthHB8I