Restriction map for
pFN21AE2041
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BcnI
BfrI BglI BlnI BlpI BmyI
BpmI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I BspDI BspEI BsrBI
BsrDI BsrFI BssHII Bst1107I BstAPI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoT22I FauI FseI FspI
HapII HgaI Hin1I HpaI HpaII
KasI KspI MfeI MluI MluNI
MroI MscI MspA1I MspI MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 77 GGTACC G^GTACC
AccI 664 GTCTAC GT^[AC][GT]AC
AciI 1641 CCGC CCGC(-3/-1)
AclI 1609 AACGTT AA^CGTT
AflIII 1619 ACATGT A^C[GA][CT]GT
Asp718I 77 GGTACC G^GTACC
BanI 77 GGTACC G^G[CT][GA]CC
BbsI 1924 GAAGAC GAAGAC(2/6)
BciVI 1398 GGATAC GTATCC(6/5)
BpuAI 1924 GAAGAC GAAGAC(2/6)
BsaAI 1119 TACGTG [CT]AC^GT[GA]
BsgI 679 CTGCAC GTGCAG(16/14)
BsmFI 307 GTCCC GGGAC(10/14)
BspLU11I 1619 ACATGT A^CATGT
BspMI 677 ACCTGC ACCTGC(4/8)
BssSI 1466 CTCGTG CACGAG(-5/-1)
BstBI 1578 TTCGAA TT^CGAA
BstXI 1844 CCAACATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1839 GCTTGC GC[ACGT]^[ACGT]GC
CfoI 1415 GCGC GCG^C
DraI 1893 TTTAAA TTT^AAA
EcoNI 569 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 861 GATATC GAT^ATC
HaeII 1414 AGCGCC [GA]GCGC^[CT]
HhaI 1415 GCGC GCG^C
HinP1I 1415 GCGC G^CGC
HincII 829 GTTGAC GT[CT]^[GA]AC
HindII 829 GTTGAC GT[CT]^[GA]AC
HindIII 1837 AAGCTT A^AGCTT
KpnI 77 GGTACC GGTAC^C
MwoI 1097 GCAAAAATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspI 1619 ACATGT [GA]CATG^[CT]
NspV 1578 TTCGAA TT^CGAA
PflMI 1389 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1609 AACGTT AA^CGTT
SapI 226 GAAGAGC GCTCTTC(1/4)
SfuI 1578 TTCGAA TT^CGAA
SmlI 373 CTTGAG C^T[CT][GA]AG
Van91I 1389 CCAAAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaII 325 GGTCC G^G[AT]CC
1147 GGACC
BclI 62 TGATCA T^GATCA
1740 TGATCA
BfaI 232 CTAG C^TAG
1678 CTAG
BglII 34 AGATCT A^GATCT
1702 AGATCT
BsaBI 1191 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1697 GATACAGATC
Bsp1407I 1656 TGTACA T^GTACA
1691 TGTACA
BspHI 1206 TCATGA T^CATGA
1239 TCATGA
BsrGI 1656 TGTACA T^GTACA
1691 TGTACA
Cfr13I 325 GGTCC G^G[ACGT]CC
1147 GGACC
EarI 1382 CTCTTC CTCTTC(1/4)
226 GAAGAG
EcoRI 621 GAATTC G^AATTC
796 GAATTC
FbaI 62 TGATCA T^GATCA
1740 TGATCA
Ksp632I 1382 CTCTTC CTCTTC(1/4)
226 GAAGAG
MaeI 232 CTAG C^TAG
1678 CTAG
MamI 1191 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1697 GATACAGATC
NcoI 852 CCATGG C^CATGG
1561 CCATGG
RcaI 1206 TCATGA T^CATGA
1239 TCATGA
Sau96I 325 GGTCC G^G[ACGT]CC
1147 GGACC
SfaNI 5 GATGC GCATC(5/9)
1191 GATGC
SspBI 1656 TGTACA T^GTACA
1691 TGTACA
StuI 377 AGGCCT AGG^CCT
1749 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbvI BsaI BsaJI BseRI
BsiYI BslI BsmAI BsrI BssKI
BstF5I BstNI BstYI DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MvaI NdeII NlaIII
NlaIV PleI RsaI Sau3AI ScrFI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI