Restriction map for
pFN21AE5821
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# NO CUT ENZYMES ( 99 enzymes) #
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AatII Acc65I AccII AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbvCI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaHI BsiEI BsiWI
BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstPI BstUI BstXI
CelII Cfr10I CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PflFI PinAI PmeI PshAI
PvuI PvuII RcaI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccIII 1352 TCCGGA T^CCGGA
AclI 2734 AACGTT AA^CGTT
AfaI 298 GTAC GT^AC
AlwNI 1162 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 2766 GGACC G^G[AT]CC
BalI 2777 TGGCCA TGG^CCA
BanII 2994 GAGCTC G[GA]GC[CT]^C
BbrPI 643 CACGTG CAC^GTG
BbsI 397 GAAGAC GAAGAC(2/6)
BcgI 2055 GCAGTTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 982 GCCTCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1677 AGATCT A^GATCT
BpuAI 397 GAAGAC GAAGAC(2/6)
BsaBI 2362 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1352 TCCGGA [AT]^CCGG[AT]
BseAI 1352 TCCGGA T^CCGGA
BseRI 2752 GAGGAG GAGGAG(10/8)
BsgI 144 GTGCAG GTGCAG(16/14)
BsmBI 2065 CGTCTC CGTCTC(1/5)
Bsp1407I 297 TGTACA T^GTACA
BspDI 1225 ATCGAT AT^CGAT
BspEI 1352 TCCGGA T^CCGGA
BspLU11I 8 ACATGT A^CATGT
BspMI 34 ACCTGC ACCTGC(4/8)
BsrGI 297 TGTACA T^GTACA
Bst1107I 323 GTATAC GTA^TAC
BstBI 1392 TTCGAA TT^CGAA
BstZ17I 323 GTATAC GTA^TAC
ClaI 1225 ATCGAT AT^CGAT
DraII 2765 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1430 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2519 CCATGG C^C[GA][CT]GG
EaeI 2777 TGGCCA [CT]^GGCC[GA]
EcoO109I 2765 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 955 GAATTC G^AATTC
EcoT14I 2519 CCATGG C^C[AT][AT]GG
HgaI 1361 GACGC GACGC(5/10)
HincII 155 GTCGAC GT[CT]^[GA]AC
HindII 155 GTCGAC GT[CT]^[GA]AC
MamI 2362 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2777 TGGCCA TGG^CCA
MroI 1352 TCCGGA T^CCGGA
MscI 2777 TGGCCA TGG^CCA
MspA1I 2980 CAGCGG C[AC]G^C[GT]G
NcoI 2519 CCATGG C^CATGG
NspI 8 ACATGT [GA]CATG^[CT]
NspV 1392 TTCGAA TT^CGAA
PaeR7I 2068 CTCGAG C^TCGAG
PflMI 174 CCAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 643 CACGTG CAC^GTG
PmlI 643 CACGTG CAC^GTG
PpuMI 2765 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 2734 AACGTT AA^CGTT
PstI 2480 CTGCAG CTGCA^G
RsaI 298 GTAC GT^AC
SacI 2994 GAGCTC GAGCT^C
SalI 155 GTCGAC G^TCGAC
SfuI 1392 TTCGAA TT^CGAA
SmaI 2769 CCCGGG CCC^GGG
SspBI 297 TGTACA T^GTACA
StyI 2519 CCATGG C^C[AT][AT]GG
Van91I 174 CCAAACGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2068 CTCGAG C^TCGAG
XmaCI 2769 CCCGGG C^CCGGG
XmaI 2769 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AccI 155 GTCGAC GT^[AC][GT]AC
323 GTATAC
AocI 378 CCTAAGG CC^T[ACGT]AGG
894 CCTCAGG
AspHI 1643 GTGCTC G[AT]GC[AT]^C
2994 GAGCTC
AvaI 2068 CTCGAG C^[CT]CG[GA]G
2769 CCCGGG
BmyI 1643 GTGCTC G[AGT]GC[ACT]^C
2994 GAGCTC
BsaAI 643 CACGTG [CT]AC^GT[GA]
2084 TACGTG
BsaI 1909 GGTCTC GGTCTC(1/5)
2612 GGTCTC
BsiHKAI 1643 GTGCTC G[AT]GC[AT]^C
2994 GAGCTC
BsmFI 2765 GGGAC GGGAC(10/14)
1824 GTCCC
BsoBI 2068 CTCGAG C^[CT]CG[GA]G
2769 CCCGGG
Bsp1286I 1643 GTGCTC G[AGT]GC[ACT]^C
2994 GAGCTC
Bsu36I 378 CCTAAGG CC^T[ACGT]AGG
894 CCTCAGG
CfoI 74 GCGC GCG^C
2703 GCGC
Cfr13I 1489 GGGCC G^G[ACGT]CC
2766 GGACC
EarI 2433 GAAGAG CTCTTC(1/4)
2716 GAAGAG
Eco81I 378 CCTAAGG CC^T[ACGT]AGG
894 CCTCAGG
FauI 1010 CCCGC CCCGC(4/6)
761 GCGGG
HhaI 74 GCGC GCG^C
2703 GCGC
HinP1I 74 GCGC G^CGC
2703 GCGC
Ksp632I 2433 GAAGAG CTCTTC(1/4)
2716 GAAGAG
Sau96I 1489 GGGCC G^G[ACGT]CC
2766 GGACC
SspI 1072 AATATT AAT^ATT
1598 AATATT
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI AseI AsnI Asp700I BbvI
BcnI BfaI BpmI BsaJI BsiYI
BslI BsmAI BsmI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII DraI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PshBI
Sau3AI ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XcmI
XhoII XmnI