Restriction map for
pFN21AE5737
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AccI AclI AcsI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
ApoI AscI AseI AsnI AspEI
AviII AvrII BamHI BbeI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsiWI BsmI BspHI
BspLU11I BspMI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
CelII CpoI DraI DraIII DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MluI NarI NcoI NheI NotI
NruI NspI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 1792 GACGTC GACGT^C
Acc65I 1739 GGTACC G^GTACC
AccIII 2150 TCCGGA T^CCGGA
AcyI 1792 GACGTC G[GA]^CG[CT]C
Asp700I 1957 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1739 GGTACC G^GTACC
AvaI 2041 CCCGAG C^[CT]CG[GA]G
BalI 1784 TGGCCA TGG^CCA
BbrPI 685 CACGTG CAC^GTG
BbvCI 2094 CCTCAGC CCTCAGC(-5/-2)
BcgI 1847 GCATGACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 685 CACGTG [CT]AC^GT[GA]
BsaHI 1792 GACGTC G[GA]^CG[CT]C
BseAI 2150 TCCGGA T^CCGGA
BseRI 1950 GAGGAG GAGGAG(10/8)
BsiEI 1294 CGGCCG CG[GA][CT]^CG
BsoBI 2041 CCCGAG C^[CT]CG[GA]G
Bsp1407I 464 TGTACA T^GTACA
BspDI 262 ATCGAT AT^CGAT
BspEI 2150 TCCGGA T^CCGGA
BsrGI 464 TGTACA T^GTACA
ClaI 262 ATCGAT AT^CGAT
DraII 2255 AGGACCC [GA]G^G[ACGT]CC[CT]
EagI 1294 CGGCCG C^GGCCG
EclXI 1294 CGGCCG C^GGCCG
Eco52I 1294 CGGCCG C^GGCCG
EcoO109I 2255 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 2143 GATATC GAT^ATC
EcoT22I 1691 ATGCAT ATGCA^T
Hin1I 1792 GACGTC G[GA]^CG[CT]C
KpnI 1739 GGTACC GGTAC^C
MfeI 812 CAATTG C^AATTG
MluNI 1784 TGGCCA TGG^CCA
MroI 2150 TCCGGA T^CCGGA
MscI 1784 TGGCCA TGG^CCA
MunI 812 CAATTG C^AATTG
NsiI 1691 ATGCAT ATGCA^T
PmaCI 685 CACGTG CAC^GTG
PmlI 685 CACGTG CAC^GTG
PpuMI 2255 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 379 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 773 CTGCAG CTGCA^G
SacI 1186 GAGCTC GAGCT^C
SfcI 773 CTGCAG C^T[GA][CT]AG
SmlI 1222 CTTGAG C^T[CT][GA]AG
SspBI 464 TGTACA T^GTACA
XbaI 834 TCTAGA T^CTAGA
XmnI 1957 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 1428 CGCG CG^CG
2183 CGCG
Alw44I 682 GTGCAC G^TGCAC
806 GTGCAC
ApaLI 682 GTGCAC G^TGCAC
806 GTGCAC
AspI 1525 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1593 GACGGTGTC
BanII 1186 GAGCTC G[GA]GC[CT]^C
1217 GAGCCC
BfaI 679 CTAG C^TAG
835 CTAG
BglI 892 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2239 GCCCAGATGGC
BsaWI 649 TCCGGT [AT]^CCGG[AT]
2150 TCCGGA
BsmBI 943 CGTCTC CGTCTC(1/5)
1867 GAGACG
BsrBI 570 GAGCGG CCGCTC(-3/-3)
2187 GAGCGG
BsrDI 1146 CATTGC GCAATG(2/0)
1549 GCAATG
BstAPI 523 GCACCATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
775 GCAGCGGCTGC
BstUI 1428 CGCG CG^CG
2183 CGCG
BstXI 1085 CCACCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1728 CCACTTAAATGG
BstYI 916 AGATCC [GA]^GATC[CT]
1388 AGATCC
DsaI 272 CCGTGG C^C[GA][CT]GG
1904 CCGTGG
EcoT14I 248 CCTTGG C^C[AT][AT]GG
660 CCTTGG
HaeII 477 GGCGCT [GA]GCGC^[CT]
1928 AGCGCC
HgaI 1869 GACGC GACGC(5/10)
2181 GACGC
MaeI 679 CTAG C^TAG
835 CTAG
MflI 916 AGATCC [GA]^GATC[CT]
1388 AGATCC
MvnI 1428 CGCG CG^CG
2183 CGCG
NaeI 1296 GCCGGC GCC^GGC
2049 GCCGGC
NgoMIV 1296 GCCGGC G^CCGGC
2049 GCCGGC
PflFI 1525 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1593 GACGGTGTC
PflMI 95 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1673 CCAGCAAGTGG
StyI 248 CCTTGG C^C[AT][AT]GG
660 CCTTGG
Tth111I 1525 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1593 GACGGTGTC
Van91I 95 CCATCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1673 CCAGCAAGTGG
XcmI 1322 CCATGTGGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1412 CCAGCATGGCTGTGG
XhoII 916 AGATCC [GA]^GATC[CT]
1388 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BbsI BbvI
BcnI BmyI BpmI BpuAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII EaeI
EarI EcoNI EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I