Restriction map for
pFN21AE5231
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# NO CUT ENZYMES ( 144 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BpuAI BsaBI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrFI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRV EcoT14I FauI
FbaI FseI FspI HindIII HpaI
HphI KasI KpnI KspI MamI
MboI MfeI MflI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI Sau3AI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp45I Tth111I XbaI XhoII
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 150 GACGTC GACGT^C
AccI 223 GTAGAC GT^[AC][GT]AC
AcsI 250 GAATTC [GA]^AATT[CT]
AfaI 102 GTAC GT^AC
AhdI 307 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 454 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 250 GAATTC [GA]^AATT[CT]
Asp700I 246 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 307 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 216 GGACC G^G[AT]CC
BciVI 22 GTATCC GTATCC(6/5)
BfaI 571 CTAG C^TAG
BlpI 447 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 447 GCTCAGC GC^T[ACGT]AGC
BsaI 412 GAGACC GGTCTC(1/5)
BsaWI 500 ACCGGA [AT]^CCGG[AT]
BseRI 442 GAGGAG GAGGAG(10/8)
BsgI 481 GTGCAG GTGCAG(16/14)
BsmAI 412 GAGAC GTCTC(1/5)
BsmFI 148 GGGAC GGGAC(10/14)
BsmI 286 GAATGC GAATGC(1/-1)
BspMI 483 GCAGGT ACCTGC(4/8)
BsrBI 120 GAGCGG CCGCTC(-3/-3)
BsrI 126 ACTGG ACTGG(1/-1)
BstF5I 617 GGATG GGATG(2/0)
CelII 447 GCTCAGC GC^T[ACGT]AGC
Cfr13I 216 GGACC G^G[ACGT]CC
DdeI 448 CTCAG C^T[ACGT]AG
EaeI 189 CGGCCA [CT]^GGCC[GA]
Eam1105I 307 GACGTGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 250 GAATTC G^AATTC
EcoT22I 535 ATGCAT ATGCA^T
FokI 617 GGATG GGATG(9/13)
HaeII 455 AGCGCC [GA]GCGC^[CT]
HaeIII 190 GGCC GG^CC
HincII 598 GTCAAC GT[CT]^[GA]AC
HindII 598 GTCAAC GT[CT]^[GA]AC
MaeI 571 CTAG C^TAG
MaeIII 56 GTAAC ^GT[ACGT]AC
MspA1I 382 CAGCTG C[AC]G^C[GT]G
NsiI 535 ATGCAT ATGCA^T
PaeR7I 394 CTCGAG C^TCGAG
PleI 401 GACTC GAGTC(4/5)
PvuII 382 CAGCTG CAG^CTG
RsaI 102 GTAC GT^AC
SapI 4 GAAGAGC GCTCTTC(1/4)
Sau96I 216 GGACC G^G[ACGT]CC
SmlI 394 CTCGAG C^T[CT][GA]AG
SnaBI 99 TACGTA TAC^GTA
Tsp509I 251 AATT ^AATT
TspRI 193 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 365 CCAACTTCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 394 CTCGAG C^TCGAG
XmnI 246 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BanI 256 GGCACC G^G[CT][GA]CC
588 GGCACC
BbvI 356 GCAGC GCAGC(8/12)
381 GCAGC
BsaAI 99 TACGTA [CT]AC^GT[GA]
220 CACGTA
BssHII 130 GCGCGC G^CGCGC
475 GCGCGC
BstNI 168 CCAGG CC^[AT]GG
459 CCTGG
EarI 403 CTCTTC CTCTTC(1/4)
4 GAAGAG
EcoRII 168 CCAGG ^CC[AT]GG
459 CCTGG
Ksp632I 403 CTCTTC CTCTTC(1/4)
4 GAAGAG
MboII 4 GAAGA GAAGA(8/7)
404 TCTTC
MvaI 168 CCAGG CC^[AT]GG
459 CCTGG
NlaIII 435 CATG CATG^
603 CATG
PflMI 453 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAACAGATGG
SfaNI 534 GATGC GCATC(5/9)
618 GATGC
SmaI 137 CCCGGG CCC^GGG
654 CCCGGG
TaqI 395 TCGA T^CGA
407 TCGA
TseI 356 GCAGC G^C[AT]GC
381 GCAGC
TthHB8I 395 TCGA T^CGA
407 TCGA
Van91I 453 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
627 CCAACAGATGG
XmaCI 137 CCCGGG C^CCGGG
654 CCCGGG
XmaI 137 CCCGGG C^CCGGG
654 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI BanII BcnI BmyI BpmI
BsaHI BsaJI BsiHKAI BsiYI BslI
BsoBI Bsp1286I BssKI BstUI Cac8I
CfoI Fnu4HI HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
MaeII MnlI MspI MvnI MwoI
NciI NlaIV SacI ScrFI TfiI