Restriction map for
pFN21AE5847
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# NO CUT ENZYMES ( 39 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI Asp718I AspEI AviII AvrII
BbvCI BglII BlnI BsiWI BstBI
CpoI Eam1105I EcoNI EcoRV FspI
HpaI KpnI MluI NheI NruI
NspV PacI PinAI PmeI Psp1406I
RsrII SacI SfiI SfuI SgrAI
SnaBI SpeI SwaI XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 4097 TCCGGA T^CCGGA
AflII 4690 CTTAAG C^TTAAG
AlwNI 225 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 3066 AGCGCT AGC^GCT
BamHI 3326 GGATCC G^GATCC
BcgI 3243 GCAGGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4690 CTTAAG C^TTAAG
BsaI 4594 GAGACC GGTCTC(1/5)
BsaWI 4097 TCCGGA [AT]^CCGG[AT]
BseAI 4097 TCCGGA T^CCGGA
Bsp1407I 3038 TGTACA T^GTACA
BspDI 2212 ATCGAT AT^CGAT
BspEI 4097 TCCGGA T^CCGGA
BsrGI 3038 TGTACA T^GTACA
BssSI 5046 CACGAG CACGAG(-5/-1)
Bst1107I 6304 GTATAC GTA^TAC
BstEII 6319 GGTTACC G^GT[ACGT]ACC
BstPI 6319 GGTTACC G^GT[ACGT]ACC
BstZ17I 6304 GTATAC GTA^TAC
ClaI 2212 ATCGAT AT^CGAT
DraI 6012 TTTAAA TTT^AAA
Eco47III 3066 AGCGCT AGC^GCT
EcoO65I 6319 GGTTACC G^GT[ACGT]ACC
EcoRI 2302 GAATTC G^AATTC
EcoT22I 4993 ATGCAT ATGCA^T
FseI 311 GGCCGGCC GGCCGG^CC
MfeI 5712 CAATTG C^AATTG
MroI 4097 TCCGGA T^CCGGA
MunI 5712 CAATTG C^AATTG
NaeI 312 GCCGGC GCC^GGC
NgoMIV 312 GCCGGC G^CCGGC
NsiI 4993 ATGCAT ATGCA^T
PaeR7I 844 CTCGAG C^TCGAG
PflMI 3306 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1641 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 3913 CGATCG CGAT^CG
SalI 337 GTCGAC G^TCGAC
SexAI 6323 ACCTGGT A^CC[AT]GGT
SspBI 3038 TGTACA T^GTACA
StuI 4 AGGCCT AGG^CCT
Van91I 3306 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1856 CCATCCTGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 844 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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Alw44I 3388 GTGCAC G^TGCAC
4474 GTGCAC
ApaI 10 GGGCCC GGGCC^C
1236 GGGCCC
ApaLI 3388 GTGCAC G^TGCAC
4474 GTGCAC
AseI 1702 ATTAAT AT^TAAT
4326 ATTAAT
AsnI 1702 ATTAAT AT^TAAT
4326 ATTAAT
Asp700I 5876 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6054 GAAACATTTC
BanII 10 GGGCCC G[GA]GC[CT]^C
1236 GGGCCC
BbrPI 1279 CACGTG CAC^GTG
1345 CACGTG
BciVI 5015 GGATAC GTATCC(6/5)
3229 GTATCC
BglI 457 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
6241 GCCATCCAGGC
BsmBI 99 CGTCTC CGTCTC(1/5)
483 CGTCTC
BsrBI 14 CCGCTC CCGCTC(-3/-3)
213 GAGCGG
BsrFI 72 GCCGGT [GA]^CCGG[CT]
312 GCCGGC
BstXI 3062 CCACAGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3077 CCACCTTCCTGG
Cfr10I 72 GCCGGT [GA]^CCGG[CT]
312 GCCGGC
DraIII 540 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
3469 CACTATGTG
DrdI 274 GACTTGCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5023 GACCAGATCGTC
HindIII 1568 AAGCTT A^AGCTT
5879 AAGCTT
NcoI 240 CCATGG C^CATGG
1674 CCATGG
NdeI 3981 CATATG CA^TATG
6521 CATATG
PmaCI 1279 CACGTG CAC^GTG
1345 CACGTG
PmlI 1279 CACGTG CAC^GTG
1345 CACGTG
PshBI 1702 ATTAAT AT^TAAT
4326 ATTAAT
SapI 5114 GAAGAGC GCTCTTC(1/4)
2619 GCTCTTC
SbfI 1005 CCTGCAGG CCTGCA^GG
2113 CCTGCAGG
ScaI 770 AGTACT AGT^ACT
2419 AGTACT
SmaI 135 CCCGGG CCC^GGG
406 CCCGGG
Sse8387I 1005 CCTGCAGG CCTGCA^GG
2113 CCTGCAGG
SspI 2446 AATATT AAT^ATT
5085 AATATT
XmaCI 135 CCCGGG C^CCGGG
406 CCCGGG
XmaI 135 CCCGGG C^CCGGG
406 CCCGGG
XmnI 5876 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6054 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 144 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AocI
ApoI AspHI AspI AvaI AvaII
BalI BanI BbeI BbsI BbvI
BclI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspLU11I
BspMI BsrDI BsrI BssHII BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI EarI EclXI Eco52I Eco81I
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NotI NspI PflFI PleI PpuMI
PstI PvuII RcaI RsaI SacII
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII