Restriction map for
pFN21AE1128
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# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MluNI MscI MslI MspA1I MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 60 TCCGGA T^CCGGA
AfaI 36 GTAC GT^AC
AluI 405 AGCT AG^CT
AlwI 354 GATCC GGATC(4/5)
ApaI 126 GGGCCC GGGCC^C
AvaII 8 GGACC G^G[AT]CC
BbsI 133 GAAGAC GAAGAC(2/6)
BbvI 314 GCAGC GCAGC(8/12)
BclI 119 TGATCA T^GATCA
BcnI 441 CCGGG CC^[GC]GG
BpuAI 133 GAAGAC GAAGAC(2/6)
BsaJI 299 CCATGG C^C[ACGT][ACGT]GG
BsaWI 60 TCCGGA [AT]^CCGG[AT]
BseAI 60 TCCGGA T^CCGGA
BsgI 337 GTGCAG GTGCAG(16/14)
BsmAI 483 GTCTC GTCTC(1/5)
BspEI 60 TCCGGA T^CCGGA
BspMI 139 ACCTGC ACCTGC(4/8)
BsrBI 322 GAGCGG CCGCTC(-3/-3)
BstNI 286 CCAGG CC^[AT]GG
BstYI 353 AGATCC [GA]^GATC[CT]
DdeI 172 CTGAG C^T[ACGT]AG
DraII 78 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 299 CCATGG C^C[GA][CT]GG
EaeI 29 TGGCCG [CT]^GGCC[GA]
EarI 91 GAAGAG CTCTTC(1/4)
EcoNI 58 CCTCCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 78 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 286 CCAGG ^CC[AT]GG
EcoT14I 299 CCATGG C^C[AT][AT]GG
FauI 4 GCGGG CCCGC(4/6)
FbaI 119 TGATCA T^GATCA
Fnu4HI 314 GCAGC GC^[ACGT]GC
HgaI 418 GCGTC GACGC(5/10)
HphI 369 GGTGA GGTGA(8/7)
ItaI 314 GCAGC GC^[ACGT]GC
Ksp632I 91 GAAGAG CTCTTC(1/4)
MaeII 410 ACGT A^CGT
MflI 353 AGATCC [GA]^GATC[CT]
MroI 60 TCCGGA T^CCGGA
MvaI 286 CCAGG CC^[AT]GG
NciI 441 CCGGG CC^[GC]GG
NcoI 299 CCATGG C^CATGG
NspI 374 GCATGC [GA]CATG^[CT]
PflMI 422 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 36 GTAC GT^AC
SfaNI 415 GATGC GCATC(5/9)
SmlI 16 CTCAAG C^T[CT][GA]AG
SphI 374 GCATGC GCATG^C
StyI 299 CCATGG C^C[AT][AT]GG
TfiI 54 GAATC G^A[AT]TC
TseI 314 GCAGC G^C[AT]GC
TspRI 50 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 422 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 353 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BanII 126 GGGCCC G[GA]GC[CT]^C
385 GAGCCC
BmyI 126 GGGCCC G[AGT]GC[ACT]^C
385 GAGCCC
Bsp1286I 126 GGGCCC G[AGT]GC[ACT]^C
385 GAGCCC
BssKI 286 CCAGG ^CC[ACGT]GG
441 CCGGG
HapII 61 CCGG C^CGG
441 CCGG
HpaII 61 CCGG C^CGG
441 CCGG
MseI 46 TTAA T^TAA
196 TTAA
MspI 61 CCGG C^CGG
441 CCGG
PleI 260 GAGTC GAGTC(4/5)
330 GAGTC
ScrFI 286 CCAGG CC^[ACGT]GG
441 CCGGG
Tru9I 46 TTAA T^TAA
196 TTAA
Tsp509I 44 AATT ^AATT
69 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AccII AciI BsiYI BslI BsmFI
BstF5I BstUI Cac8I CfoI Cfr13I
DpnII FokI HaeIII HhaI HinP1I
HinfI MboI MboII MnlI MvnI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I