Restriction map for
pFN21AE3075
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# NO CUT ENZYMES ( 171 enzymes) #
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AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiEI
BsiWI BsmAI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI Hin1I
HindIII HpaI HpaII KasI KspI
MaeII MamI MfeI MluI MluNI
MroI MscI MseI MspI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 74 GGTACC G^GTACC
AlwI 669 GATCC GGATC(4/5)
Asp718I 74 GGTACC G^GTACC
AspHI 88 GAGCTC G[AT]GC[AT]^C
AvaII 406 GGACC G^G[AT]CC
BanI 74 GGTACC G^G[CT][GA]CC
BanII 88 GAGCTC G[GA]GC[CT]^C
BbsI 43 GTCTTC GAAGAC(2/6)
BfaI 557 CTAG C^TAG
BmyI 88 GAGCTC G[AGT]GC[ACT]^C
BpmI 164 CTCCAG CTGGAG(16/14)
BpuAI 43 GTCTTC GAAGAC(2/6)
BsiHKAI 88 GAGCTC G[AT]GC[AT]^C
BsmFI 169 GTCCC GGGAC(10/14)
BsmI 504 GAATGC GAATGC(1/-1)
Bsp1286I 88 GAGCTC G[AGT]GC[ACT]^C
BspMI 77 ACCTGC ACCTGC(4/8)
BsrDI 330 CATTGC GCAATG(2/0)
BssKI 62 CCTGG ^CC[ACGT]GG
BstEII 459 GGTGACC G^GT[ACGT]ACC
BstF5I 129 CATCC GGATG(2/0)
BstNI 62 CCTGG CC^[AT]GG
BstPI 459 GGTGACC G^GT[ACGT]ACC
BstYI 668 AGATCC [GA]^GATC[CT]
CfoI 3 GCGC GCG^C
Cfr13I 406 GGACC G^G[ACGT]CC
EarI 451 CTCTTC CTCTTC(1/4)
EcoNI 21 CCTGCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 459 GGTGACC G^GT[ACGT]ACC
EcoRII 62 CCTGG ^CC[AT]GG
FokI 129 CATCC GGATG(9/13)
HhaI 3 GCGC GCG^C
HinP1I 3 GCGC G^CGC
HincII 340 GTCAAC GT[CT]^[GA]AC
HindII 340 GTCAAC GT[CT]^[GA]AC
HinfI 198 GACTC G^A[ACGT]TC
HphI 459 GGTGA GGTGA(8/7)
KpnI 74 GGTACC GGTAC^C
Ksp632I 451 CTCTTC CTCTTC(1/4)
MaeI 557 CTAG C^TAG
MflI 668 AGATCC [GA]^GATC[CT]
MslI 625 CACTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 62 CCTGG CC^[AT]GG
NlaIV 74 GGTACC GG[ACGT]^[ACGT]CC
PflMI 7 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 198 GACTC GAGTC(4/5)
PstI 662 CTGCAG CTGCA^G
PvuII 301 CAGCTG CAG^CTG
SacI 88 GAGCTC GAGCT^C
SapI 450 GCTCTTC GCTCTTC(1/4)
Sau96I 406 GGACC G^G[ACGT]CC
ScrFI 62 CCTGG CC^[ACGT]GG
SmlI 25 CTCAAG C^T[CT][GA]AG
StuI 148 AGGCCT AGG^CCT
Van91I 7 CCATTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 668 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 132 CCGC CCGC(-3/-1)
239 GCGG
AcsI 547 AAATTC [GA]^AATT[CT]
602 AAATTC
AfaI 75 GTAC GT^AC
258 GTAC
ApoI 547 AAATTC [GA]^AATT[CT]
602 AAATTC
DdeI 235 CTCAG C^T[ACGT]AG
635 CTAAG
DpnII 618 GATC ^GATC
669 GATC
MboI 618 GATC ^GATC
669 GATC
MboII 44 TCTTC GAAGA(8/7)
452 TCTTC
MspA1I 237 CAGCGG C[AC]G^C[GT]G
301 CAGCTG
NdeII 618 GATC ^GATC
669 GATC
NlaIII 456 CATG CATG^
543 CATG
RsaI 75 GTAC GT^AC
258 GTAC
Sau3AI 618 GATC ^GATC
669 GATC
Tsp509I 548 AATT ^AATT
603 AATT
TspRI 114 CAGTG CA[GC]TG[ACGT][ACGT]^
193 CACTG
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BbvI BsiYI BslI BsrI
Cac8I Fnu4HI HaeIII ItaI MaeIII
MnlI MwoI SfaNI SfcI TseI
Tsp45I