Restriction map for
pFN21AE4154
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# NO CUT ENZYMES ( 103 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AvaI AvrII BamHI BbrPI
BbvCI BciVI BfaI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsiWI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
DraI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MseI MunI NaeI
NdeI NgoMIV NheI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 541 TCCGGA T^CCGGA
Alw44I 1500 GTGCAC G^TGCAC
AlwI 597 GGATC GGATC(4/5)
ApaLI 1500 GTGCAC G^TGCAC
AspI 226 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 677 TGCGCA TGC^GCA
BalI 1100 TGGCCA TGG^CCA
BcgI 993 CGAGCTCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1238 TGATCA T^GATCA
BglII 1346 AGATCT A^GATCT
BsaWI 541 TCCGGA [AT]^CCGG[AT]
BseAI 541 TCCGGA T^CCGGA
BspEI 541 TCCGGA T^CCGGA
BspHI 1121 TCATGA T^CATGA
BsrBI 1327 GAGCGG CCGCTC(-3/-3)
BsrFI 41 ACCGGC [GA]^CCGG[CT]
BsrI 1287 CCAGT ACTGG(1/-1)
BssHII 1110 GCGCGC G^CGCGC
BssSI 1622 CACGAG CACGAG(-5/-1)
BstYI 1346 AGATCT [GA]^GATC[CT]
Cfr10I 41 ACCGGC [GA]^CCGG[CT]
DraIII 1492 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 943 GACCTGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 688 GAAGAG CTCTTC(1/4)
EcoNI 1080 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1238 TGATCA T^GATCA
FspI 677 TGCGCA TGC^GCA
Ksp632I 688 GAAGAG CTCTTC(1/4)
MflI 1346 AGATCT [GA]^GATC[CT]
MluNI 1100 TGGCCA TGG^CCA
MroI 541 TCCGGA T^CCGGA
MscI 1100 TGGCCA TGG^CCA
NcoI 1542 CCATGG C^CATGG
NotI 1716 GCGGCCGC GC^GGCCGC
NspI 1307 ACATGC [GA]CATG^[CT]
PflFI 226 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1389 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 561 CAGCTG CAG^CTG
RcaI 1121 TCATGA T^CATGA
SapI 688 GAAGAGC GCTCTTC(1/4)
SfiI 477 GGCCGTGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1297 CTCAAG C^T[CT][GA]AG
TfiI 813 GAATC G^A[AT]TC
Tsp509I 535 AATT ^AATT
TspRI 1288 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 226 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1389 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1346 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AocI 1612 CCTGAGG CC^T[ACGT]AGG
1669 CCTGAGG
BbeI 439 GGCGCC GGCGC^C
1487 GGCGCC
BglI 478 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
642 GCCTCTATGGC
BsaI 829 GAGACC GGTCTC(1/5)
1018 GGTCTC
BsiEI 476 CGGCCG CG[GA][CT]^CG
1717 CGGCCG
BsmBI 704 CGTCTC CGTCTC(1/5)
656 GAGACG
BsmFI 33 GTCCC GGGAC(10/14)
1218 GTCCC
BstAPI 45 GCACCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1427 GCAAGGTGTGC
BstXI 1037 CCAGCTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1091 CCACCGCGGTGG
Bsu36I 1612 CCTGAGG CC^T[ACGT]AGG
1669 CCTGAGG
EagI 476 CGGCCG C^GGCCG
1717 CGGCCG
EclXI 476 CGGCCG C^GGCCG
1717 CGGCCG
Eco52I 476 CGGCCG C^GGCCG
1717 CGGCCG
Eco81I 1612 CCTGAGG CC^T[ACGT]AGG
1669 CCTGAGG
FauI 54 GCGGG CCCGC(4/6)
1352 GCGGG
KasI 439 GGCGCC G^GCGCC
1487 GGCGCC
MslI 306 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1092 CACCGCGGTG
NarI 439 GGCGCC GG^CGCC
1487 GGCGCC
PpuMI 1607 AGGACCC [GA]G^G[AT]CC[CT]
1616 AGGACCC
PstI 334 CTGCAG CTGCA^G
1266 CTGCAG
SacI 58 GAGCTC GAGCT^C
994 GAGCTC
SfcI 334 CTGCAG C^T[GA][CT]AG
1266 CTGCAG
SfoI 439 GGCGCC GGC^GCC
1487 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI ApaI Asp700I AspHI AvaII
BanI BanII BbsI BbvI BcnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI SacII Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp45I TthHB8I XmnI