Restriction map for
pFN21AE2246
##################################
# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI AlwI
AocI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DpnII DraI DrdI DsaI Eam1105I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI Hin1I
HincII HindII HindIII HinfI HpaI
HphI KasI KspI MaeI MaeIII
MamI MboI MfeI MflI MluI
MroI MseI MunI NarI NcoI
NdeI NdeII NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
Sau3AI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I Van91I XbaI
XcmI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 612 GGTACC G^GTACC
AflIII 205 ACGTGT A^C[GA][CT]GT
Asp718I 612 GGTACC G^GTACC
AvaII 727 GGTCC G^G[AT]CC
BalI 845 TGGCCA TGG^CCA
BanII 787 GAGCTC G[GA]GC[CT]^C
BsiEI 178 CGGCCG CG[GA][CT]^CG
BsmFI 116 GGGAC GGGAC(10/14)
BstF5I 851 GGATG GGATG(2/0)
EagI 178 CGGCCG C^GGCCG
EarI 555 CTCTTC CTCTTC(1/4)
EclXI 178 CGGCCG C^GGCCG
Eco52I 178 CGGCCG C^GGCCG
EcoT14I 736 CCAAGG C^C[AT][AT]GG
FokI 851 GGATG GGATG(9/13)
KpnI 612 GGTACC GGTAC^C
Ksp632I 555 CTCTTC CTCTTC(1/4)
MluNI 845 TGGCCA TGG^CCA
MscI 845 TGGCCA TGG^CCA
NlaIII 565 CATG CATG^
PaeR7I 706 CTCGAG C^TCGAG
SacI 787 GAGCTC GAGCT^C
SapI 554 GCTCTTC GCTCTTC(1/4)
SexAI 399 ACCTGGT A^CC[AT]GGT
SfiI 702 GGCCCTCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 706 CTCGAG C^T[CT][GA]AG
StuI 21 AGGCCT AGG^CCT
StyI 736 CCAAGG C^C[AT][AT]GG
XhoI 706 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 79 GTAC GT^AC
613 GTAC
Alw44I 576 GTGCAC G^TGCAC
895 GTGCAC
Aor51HI 257 AGCGCT AGC^GCT
881 AGCGCT
ApaLI 576 GTGCAC G^TGCAC
895 GTGCAC
BbvCI 600 CCTCAGC CCTCAGC(-5/-2)
622 GCTGAGG
BsmAI 124 GAGAC GTCTC(1/5)
191 GAGAC
BsrBI 133 GAGCGG CCGCTC(-3/-3)
865 GAGCGG
BsrI 589 ACTGG ACTGG(1/-1)
150 CCAGT
DraII 701 AGGCCCT [GA]G^G[ACGT]CC[CT]
710 AGGCCCC
EaeI 178 CGGCCG [CT]^GGCC[GA]
845 TGGCCA
Eco47III 257 AGCGCT AGC^GCT
881 AGCGCT
EcoO109I 701 AGGCCCT [GA]G^G[ACGT]CC[CT]
710 AGGCCCC
MaeII 205 ACGT A^CGT
275 ACGT
MboII 356 TCTTC GAAGA(8/7)
556 TCTTC
MslI 165 CACCTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
325 CACAACCGTG
NaeI 72 GCCGGC GCC^GGC
406 GCCGGC
NgoMIV 72 GCCGGC G^CCGGC
406 GCCGGC
PvuII 582 CAGCTG CAG^CTG
760 CAGCTG
RsaI 79 GTAC GT^AC
613 GTAC
SfaNI 852 GATGC GCATC(5/9)
388 GCATC
SmaI 420 CCCGGG CCC^GGG
856 CCCGGG
TaqI 647 TCGA T^CGA
707 TCGA
TthHB8I 647 TCGA T^CGA
707 TCGA
XmaCI 420 CCCGGG C^CCGGG
856 CCCGGG
XmaI 420 CCCGGG C^CCGGG
856 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaI BanI BbvI BcnI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrFI BssKI BstAPI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraIII EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
PstI Sau96I ScrFI SfcI TseI
TspRI