Restriction map for
pFN21AE1983
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BcgI BciVI BfrI BglI BglII
BlnI BsaBI BseAI BsiEI BsiWI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrDI BssHII Bst1107I BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV FseI FspI HincII HindII
HpaI KasI KpnI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SalI SbfI
ScaI SfcI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcyI 100 GACGCC G[GA]^CG[CT]C
AlwNI 575 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 279 AGCGCT AGC^GCT
Asp700I 1257 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1249 GAAGAC GAAGAC(2/6)
BbvCI 515 CCTCAGC CCTCAGC(-5/-2)
BfaI 1246 CTAG C^TAG
BlpI 1332 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1332 GCTGAGC GC^T[ACGT]AGC
BpuAI 1249 GAAGAC GAAGAC(2/6)
BsaAI 1510 TACGTG [CT]AC^GT[GA]
BsaHI 100 GACGCC G[GA]^CG[CT]C
BsaWI 1253 ACCGGA [AT]^CCGG[AT]
BsgI 889 CTGCAC GTGCAG(16/14)
BsmBI 433 GAGACG CGTCTC(1/5)
BsmFI 481 GGGAC GGGAC(10/14)
Bsp1407I 402 TGTACA T^GTACA
BspMI 1344 ACCTGC ACCTGC(4/8)
BsrGI 402 TGTACA T^GTACA
BssSI 31 CTCGTG CACGAG(-5/-1)
BstAPI 368 GCAAAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 170 TTCGAA TT^CGAA
BstXI 653 CCAGACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1332 GCTGAGC GC^T[ACGT]AGC
DraII 390 AGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 279 AGCGCT AGC^GCT
EcoO109I 390 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1640 ATGCAT ATGCA^T
Hin1I 100 GACGCC G[GA]^CG[CT]C
KspI 95 CCGCGG CCGC^GG
MaeI 1246 CTAG C^TAG
MslI 1184 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 298 GCCGGC GCC^GGC
NgoMIV 298 GCCGGC G^CCGGC
NsiI 1640 ATGCAT ATGCA^T
NspI 1638 ACATGC [GA]CATG^[CT]
NspV 170 TTCGAA TT^CGAA
PpuMI 390 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 518 CAGCTG CAG^CTG
SacII 95 CCGCGG CCGC^GG
SapI 1354 GAAGAGC GCTCTTC(1/4)
SexAI 488 ACCAGGT A^CC[AT]GGT
SfuI 170 TTCGAA TT^CGAA
SspBI 402 TGTACA T^GTACA
XcmI 615 CCAAGCCTTAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1257 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 1104 AAATTC [GA]^AATT[CT]
1487 AAATTC
ApoI 1104 AAATTC [GA]^AATT[CT]
1487 AAATTC
AspI 270 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
487 GACCAGGTC
BclI 1097 TGATCA T^GATCA
1392 TGATCA
DrdI 58 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1563 GACGAGACTGTC
EcoT14I 470 CCTTGG C^C[AT][AT]GG
716 CCAAGG
FauI 39 GCGGG CCCGC(4/6)
1002 GCGGG
FbaI 1097 TGATCA T^GATCA
1392 TGATCA
HindIII 1318 AAGCTT A^AGCTT
1444 AAGCTT
MaeIII 56 GTGAC ^GT[ACGT]AC
745 GTCAC
MspA1I 95 CCGCGG C[AC]G^C[GT]G
518 CAGCTG
NlaIII 780 CATG CATG^
1639 CATG
PflFI 270 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
487 GACCAGGTC
StyI 470 CCTTGG C^C[AT][AT]GG
716 CCAAGG
Tsp45I 56 GTGAC ^GT[GC]AC
745 GTCAC
Tth111I 270 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
487 GACCAGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MluNI MnlI MscI MseI
MspI MvaI MvnI MwoI NciI
NdeII NlaIV PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII