Restriction map for
pFN21AB6958
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbsI BbvCI BciVI
BclI BfaI BfrI BlnI BlpI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI Tru9I Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 532 GACGTC GACGT^C
AccI 322 GTCTAC GT^[AC][GT]AC
AccII 91 CGCG CG^CG
AcyI 532 GACGTC G[GA]^CG[CT]C
AlwNI 282 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 260 GGGCCC GGGCC^C
AspHI 268 GAGCAC G[AT]GC[AT]^C
BanI 372 GGTGCC G^G[CT][GA]CC
BbrPI 291 CACGTG CAC^GTG
BglI 253 GCCGAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 121 AGATCT A^GATCT
BsaAI 291 CACGTG [CT]AC^GT[GA]
BsaHI 532 GACGTC G[GA]^CG[CT]C
BseRI 163 GAGGAG GAGGAG(10/8)
BsiEI 251 CGGCCG CG[GA][CT]^CG
BsiHKAI 268 GAGCAC G[AT]GC[AT]^C
BspMI 679 ACCTGC ACCTGC(4/8)
BsrI 69 CCAGT ACTGG(1/-1)
BstUI 91 CGCG CG^CG
BstYI 121 AGATCT [GA]^GATC[CT]
EagI 251 CGGCCG C^GGCCG
EclXI 251 CGGCCG C^GGCCG
Eco52I 251 CGGCCG C^GGCCG
EcoNI 693 CCTCTTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 284 GGCGCT [GA]GCGC^[CT]
Hin1I 532 GACGTC G[GA]^CG[CT]C
HindIII 402 AAGCTT A^AGCTT
HphI 299 TCACC GGTGA(8/7)
MaeIII 37 GTGAC ^GT[ACGT]AC
MflI 121 AGATCT [GA]^GATC[CT]
MspA1I 687 CCGCTG C[AC]G^C[GT]G
MvnI 91 CGCG CG^CG
NlaIII 645 CATG CATG^
PleI 659 GACTC GAGTC(4/5)
PmaCI 291 CACGTG CAC^GTG
PmlI 291 CACGTG CAC^GTG
SfaNI 79 GATGC GCATC(5/9)
SfiI 252 GGCCGAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 500 AGGCCT AGG^CCT
Tsp45I 37 GTGAC ^GT[GC]AC
Tsp509I 544 AATT ^AATT
XhoII 121 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 278 GATCC GGATC(4/5)
350 GGATC
AvaII 217 GGTCC G^G[AT]CC
600 GGACC
BcgI 203 CGACAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
653 GCAGTGGACTCG
BsgI 288 CTGCAC GTGCAG(16/14)
508 CTGCAC
BsmAI 142 GAGAC GTCTC(1/5)
166 GAGAC
BstF5I 634 CATCC GGATG(2/0)
78 GGATG
DdeI 125 CTGAG C^T[ACGT]AG
266 CTGAG
EaeI 251 CGGCCG [CT]^GGCC[GA]
460 CGGCCA
EarI 139 GAAGAG CTCTTC(1/4)
574 GAAGAG
FauI 686 CCCGC CCCGC(4/6)
424 GCGGG
FokI 634 CATCC GGATG(9/13)
78 GGATG
Ksp632I 139 GAAGAG CTCTTC(1/4)
574 GAAGAG
MaeII 292 ACGT A^CGT
533 ACGT
MboII 139 GAAGA GAAGA(8/7)
574 GAAGA
PflMI 220 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
522 CCAAACTCTGG
TfiI 53 GATTC G^A[AT]TC
441 GAATC
Van91I 220 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
522 CCAAACTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MboI MnlI
MspI MvaI MwoI NciI NdeII
NlaIV PstI Sau3AI Sau96I ScrFI
SfcI TseI TspRI