Restriction map for
pFN21AE5634
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbeI BbrPI BbvCI BclI
BfaI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MaeI MamI
MfeI MroI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SacII ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccII 78 CGCG CG^CG
AflII 961 CTTAAG C^TTAAG
AflIII 77 ACGCGT A^C[GA][CT]GT
Alw44I 1572 GTGCAC G^TGCAC
AlwNI 1451 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1572 GTGCAC G^TGCAC
BalI 176 TGGCCA TGG^CCA
BamHI 504 GGATCC G^GATCC
BanII 1491 GGGCTC G[GA]GC[CT]^C
BciVI 1723 GTATCC GTATCC(6/5)
BfrI 961 CTTAAG C^TTAAG
BglI 643 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 1268 CTGCAC GTGCAG(16/14)
BspHI 860 TCATGA T^CATGA
BspMI 1068 ACCTGC ACCTGC(4/8)
BstAPI 262 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 4 GGTAACC G^GT[ACGT]ACC
BstPI 4 GGTAACC G^GT[ACGT]ACC
BstUI 78 CGCG CG^CG
BstXI 999 CCAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 504 GGATCC [GA]^GATC[CT]
DsaI 1586 CCACGG C^C[GA][CT]GG
EaeI 176 TGGCCA [CT]^GGCC[GA]
EarI 1536 GAAGAG CTCTTC(1/4)
EcoO65I 4 GGTAACC G^GT[ACGT]ACC
EcoRI 1014 GAATTC G^AATTC
HgaI 79 GCGTC GACGC(5/10)
HindIII 358 AAGCTT A^AGCTT
Ksp632I 1536 GAAGAG CTCTTC(1/4)
MaeII 315 ACGT A^CGT
MflI 504 GGATCC [GA]^GATC[CT]
MluI 77 ACGCGT A^CGCGT
MluNI 176 TGGCCA TGG^CCA
MscI 176 TGGCCA TGG^CCA
MvnI 78 CGCG CG^CG
NlaIII 861 CATG CATG^
PshAI 1343 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 860 TCATGA T^CATGA
SapI 1536 GAAGAGC GCTCTTC(1/4)
SbfI 712 CCTGCAGG CCTGCA^GG
Sse8387I 712 CCTGCAGG CCTGCA^GG
StuI 1674 AGGCCT AGG^CCT
XcmI 400 CCAAGTGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 504 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 111 GTCGAC GT^[AC][GT]AC
142 GTCGAC
AcsI 586 AAATTC [GA]^AATT[CT]
1014 GAATTC
AfaI 307 GTAC GT^AC
317 GTAC
ApoI 586 AAATTC [GA]^AATT[CT]
1014 GAATTC
AspHI 1469 GAGCAC G[AT]GC[AT]^C
1572 GTGCAC
AspI 481 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1153 GACTCAGTC
AvaI 190 CCCGAG C^[CT]CG[GA]G
256 CCCGAG
BbsI 34 GAAGAC GAAGAC(2/6)
919 GAAGAC
BcgI 1257 CGAAGGAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1598 CGAACTCACTGC
BcnI 436 CCGGG CC^[GC]GG
471 CCCGG
BpuAI 34 GAAGAC GAAGAC(2/6)
919 GAAGAC
BsaWI 319 ACCGGA [AT]^CCGG[AT]
420 TCCGGT
BsiHKAI 1469 GAGCAC G[AT]GC[AT]^C
1572 GTGCAC
BsoBI 190 CCCGAG C^[CT]CG[GA]G
256 CCCGAG
DraI 871 TTTAAA TTT^AAA
1669 TTTAAA
EcoNI 304 CCTGTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
523 CCTGAGACAGG
NciI 436 CCGGG CC^[GC]GG
471 CCCGG
PflFI 481 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1153 GACTCAGTC
PstI 713 CTGCAG CTGCA^G
1606 CTGCAG
RsaI 307 GTAC GT^AC
317 GTAC
SalI 111 GTCGAC G^TCGAC
142 GTCGAC
SmlI 961 CTTAAG C^T[CT][GA]AG
1703 CTCAAG
Tth111I 481 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1153 GACTCAGTC
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AhdI AluI AlwI AspEI
AvaII BanI BbvI BmyI BpmI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII Eam1105I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HincII HindII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIV PleI PpuMI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I