Restriction map for
pFN21AE5015
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BseRI BsgI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I ClaI
CpoI DpnII DraI EarI Eco47III
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
Ksp632I KspI MaeI MamI MboI
MfeI MflI MluI MluNI MscI
MseI MslI MunI NcoI NdeI
NdeII NheI NruI NsiI NspV
PacI PflFI PinAI PmeI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccIII 143 TCCGGA T^CCGGA
AhdI 116 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 971 GGGCCC GGGCC^C
AspEI 116 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 874 CACGTG CAC^GTG
BcgI 762 CGAGCGCGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 205 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 205 GCTGAGC GC^T[ACGT]AGC
BsaWI 143 TCCGGA [AT]^CCGG[AT]
BseAI 143 TCCGGA T^CCGGA
BspEI 143 TCCGGA T^CCGGA
BspMI 53 ACCTGC ACCTGC(4/8)
BsrFI 220 GCCGGC [GA]^CCGG[CT]
CelII 205 GCTGAGC GC^T[ACGT]AGC
Cfr10I 220 GCCGGC [GA]^CCGG[CT]
DdeI 206 CTGAG C^T[ACGT]AG
DraII 970 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 42 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 453 GACGTGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 116 GACTGCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 970 GGGGCCC [GA]G^G[ACGT]CC[CT]
MroI 143 TCCGGA T^CCGGA
MspA1I 355 CCGCTG C[AC]G^C[GT]G
NaeI 220 GCCGGC GCC^GGC
NgoMIV 220 GCCGGC G^CCGGC
NlaIII 330 CATG CATG^
NotI 104 GCGGCCGC GC^GGCCGC
NspI 329 GCATGT [GA]CATG^[CT]
PflMI 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 874 CACGTG CAC^GTG
PmlI 874 CACGTG CAC^GTG
PshAI 360 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI 350 GCATC GCATC(5/9)
SfiI 513 GGCCCTCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Tsp45I 367 GTCAC ^GT[GC]AC
Van91I 375 CCAGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 285 GTAC GT^AC
305 GTAC
Alw44I 39 GTGCAC G^TGCAC
877 GTGCAC
AlwNI 112 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
376 CAGAGCCTG
ApaLI 39 GTGCAC G^TGCAC
877 GTGCAC
AspHI 39 GTGCAC G[AT]GC[AT]^C
877 GTGCAC
BglI 505 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
514 GCCCTCGGGGC
BsaAI 658 TACGTG [CT]AC^GT[GA]
874 CACGTG
BsiHKAI 39 GTGCAC G[AT]GC[AT]^C
877 GTGCAC
BsrBI 843 CCGCTC CCGCTC(-3/-3)
627 GAGCGG
BsrI 755 ACTGG ACTGG(1/-1)
228 CCAGT
BssHII 18 GCGCGC G^CGCGC
765 GCGCGC
BstF5I 351 CATCC GGATG(2/0)
681 CATCC
FokI 351 CATCC GGATG(9/13)
681 CATCC
HgaI 79 GACGC GACGC(5/10)
360 GACGC
HinfI 88 GAGTC G^A[ACGT]TC
310 GACTC
MaeIII 50 GTTAC ^GT[ACGT]AC
367 GTCAC
PaeR7I 85 CTCGAG C^TCGAG
927 CTCGAG
PleI 310 GACTC GAGTC(4/5)
88 GAGTC
PstI 622 CTGCAG CTGCA^G
640 CTGCAG
RsaI 285 GTAC GT^AC
305 GTAC
SexAI 473 ACCTGGT A^CC[AT]GGT
719 ACCTGGT
SfcI 622 CTGCAG C^T[GA][CT]AG
640 CTGCAG
SmaI 795 CCCGGG CCC^GGG
933 CCCGGG
SmlI 85 CTCGAG C^T[CT][GA]AG
927 CTCGAG
TspRI 229 CAGTG CA[GC]TG[ACGT][ACGT]^
620 CACTG
XcmI 428 CCAAGTGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
464 CCACGGAGAACCTGG
XhoI 85 CTCGAG C^TCGAG
927 CTCGAG
XmaCI 795 CCCGGG C^CCGGG
933 CCCGGG
XmaI 795 CCCGGG C^CCGGG
933 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AcyI AluI AvaI
AvaII BanI BanII BbeI BbsI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaJI BsiEI BsiYI BslI
BsoBI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DsaI EaeI
EagI EclXI Eco52I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MboII MnlI MspI
MvaI MvnI MwoI NarI NciI
NlaIV Sau96I ScrFI SfoI TaqI
TseI TthHB8I