Restriction map for
pFN21AB7931
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwNI
AocI ApoI AscI AseI AsnI
Asp718I AviII BalI BamHI BbrPI
BbvCI BclI BfrI BglII BlpI
Bpu1102I BsaAI BsaBI BseAI BsgI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeIII MamI MfeI MluI
MluNI MroI MscI MseI MunI
NdeI NheI NruI NspI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp45I Tsp509I
Van91I XbaI XcmI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcyI 132 GGCGCC G[GA]^CG[CT]C
AhdI 430 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1015 GTGCAC G^TGCAC
Aor51HI 671 AGCGCT AGC^GCT
ApaLI 1015 GTGCAC G^TGCAC
Asp700I 540 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 430 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 406 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 67 CCTAGG C^CTAGG
BbeI 132 GGCGCC GGCGC^C
BciVI 620 GGATAC GTATCC(6/5)
BlnI 67 CCTAGG C^CTAGG
BsaHI 132 GGCGCC G[GA]^CG[CT]C
BsaI 1003 GAGACC GGTCTC(1/5)
BsaWI 617 ACCGGA [AT]^CCGG[AT]
BseRI 250 CTCCTC GAGGAG(10/8)
BsmBI 411 CGTCTC CGTCTC(1/5)
BsmI 544 GCATTC GAATGC(1/-1)
BspMI 104 ACCTGC ACCTGC(4/8)
BsrBI 284 CCGCTC CCGCTC(-3/-3)
BssHII 791 GCGCGC G^CGCGC
BstXI 1052 CCACGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 154 GGATCT [GA]^GATC[CT]
Eam1105I 430 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 529 GAAGAG CTCTTC(1/4)
Eco47III 671 AGCGCT AGC^GCT
EcoT22I 811 ATGCAT ATGCA^T
HgaI 410 GCGTC GACGC(5/10)
Hin1I 132 GGCGCC G[GA]^CG[CT]C
HphI 519 TCACC GGTGA(8/7)
KasI 132 GGCGCC G^GCGCC
Ksp632I 529 GAAGAG CTCTTC(1/4)
MflI 154 GGATCT [GA]^GATC[CT]
MslI 825 CATGGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 132 GGCGCC GG^CGCC
NotI 280 GCGGCCGC GC^GGCCGC
NsiI 811 ATGCAT ATGCA^T
PaeR7I 287 CTCGAG C^TCGAG
PflFI 406 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 523 CAGCTG CAG^CTG
SacI 604 GAGCTC GAGCT^C
SfoI 132 GGCGCC GGC^GCC
SgrAI 275 CGCCGGCG C[GA]^CCGG[CT]G
TaqI 288 TCGA T^CGA
Tth111I 406 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 288 TCGA T^CGA
XhoI 287 CTCGAG C^TCGAG
XhoII 154 GGATCT [GA]^GATC[CT]
XmnI 540 GAAGGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 702 GTAC GT^AC
711 GTAC
AlwI 154 GGATC GGATC(4/5)
919 GGATC
AvaII 102 GGACC G^G[AT]CC
169 GGTCC
BanI 51 GGTGCC G^G[CT][GA]CC
132 GGCGCC
BbsI 442 GAAGAC GAAGAC(2/6)
613 GAAGAC
BcgI 289 CGAGCACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
342 CGAGCCCGCTGC
BpuAI 442 GAAGAC GAAGAC(2/6)
613 GAAGAC
BsmAI 1003 GAGAC GTCTC(1/5)
412 GTCTC
BsmFI 11 GGGAC GGGAC(10/14)
240 GGGAC
BsrI 1059 ACTGG ACTGG(1/-1)
435 CCAGT
DpnII 155 GATC ^GATC
920 GATC
MaeII 314 ACGT A^CGT
418 ACGT
MboI 155 GATC ^GATC
920 GATC
NaeI 129 GCCGGC GCC^GGC
276 GCCGGC
NcoI 719 CCATGG C^CATGG
824 CCATGG
NdeII 155 GATC ^GATC
920 GATC
NgoMIV 129 GCCGGC G^CCGGC
276 GCCGGC
NlaIII 720 CATG CATG^
825 CATG
RsaI 702 GTAC GT^AC
711 GTAC
Sau3AI 155 GATC ^GATC
920 GATC
SmlI 287 CTCGAG C^T[CT][GA]AG
1034 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiEI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrFI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
PleI Sau96I ScrFI SfaNI SmaI
StyI TseI TspRI XmaCI XmaI