Restriction map for
pFN21AE3135
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI BspDI
BspEI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII KasI
KpnI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 106 CGCG CG^CG
AlwNI 887 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 506 GAGCAC G[AT]GC[AT]^C
AvaI 187 CCCGAG C^[CT]CG[GA]G
AvaII 54 GGTCC G^G[AT]CC
BanII 16 GGGCTC G[GA]GC[CT]^C
BglI 9 GCCGGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 905 AGATCT A^GATCT
BseRI 217 GAGGAG GAGGAG(10/8)
BsiHKAI 506 GAGCAC G[AT]GC[AT]^C
BsoBI 187 CCCGAG C^[CT]CG[GA]G
BspHI 933 TCATGA T^CATGA
BspMI 891 ACCTGC ACCTGC(4/8)
BsrFI 9 GCCGGC [GA]^CCGG[CT]
BsrI 170 ACTGG ACTGG(1/-1)
BstAPI 361 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 922 GGATG GGATG(2/0)
BstUI 106 CGCG CG^CG
CfoI 24 GCGC GCG^C
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
Cfr13I 54 GGTCC G^G[ACGT]CC
DdeI 384 CTCAG C^T[ACGT]AG
DraI 531 TTTAAA TTT^AAA
DraII 53 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 105 CCGCGG C^C[GA][CT]GG
EagI 7 CGGCCG C^GGCCG
EarI 296 GAAGAG CTCTTC(1/4)
EclXI 7 CGGCCG C^GGCCG
Eco52I 7 CGGCCG C^GGCCG
EcoNI 210 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 53 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 427 GAATTC G^AATTC
EcoT14I 40 CCTTGG C^C[AT][AT]GG
FauI 104 CCCGC CCCGC(4/6)
FokI 922 GGATG GGATG(9/13)
HaeII 23 GGCGCT [GA]GCGC^[CT]
HhaI 24 GCGC GCG^C
HinP1I 24 GCGC G^CGC
HincII 736 GTTAAC GT[CT]^[GA]AC
HindII 736 GTTAAC GT[CT]^[GA]AC
HpaI 736 GTTAAC GTT^AAC
Ksp632I 296 GAAGAG CTCTTC(1/4)
KspI 105 CCGCGG CCGC^GG
MvnI 106 CGCG CG^CG
NaeI 9 GCCGGC GCC^GGC
NgoMIV 9 GCCGGC G^CCGGC
PleI 937 GAGTC GAGTC(4/5)
PpuMI 53 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 933 TCATGA T^CATGA
SacII 105 CCGCGG CCGC^GG
Sau96I 54 GGTCC G^G[ACGT]CC
SexAI 458 ACCTGGT A^CC[AT]GGT
SfaNI 822 GCATC GCATC(5/9)
SmlI 511 CTTGAG C^T[CT][GA]AG
StyI 40 CCTTGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 233 GGATC GGATC(4/5)
282 GGATC
Asp700I 132 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
427 GAATTCCTTC
BfaI 503 CTAG C^TAG
991 CTAG
BmyI 16 GGGCTC G[AGT]GC[ACT]^C
506 GAGCAC
BsiEI 7 CGGCCG CG[GA][CT]^CG
141 CGACCG
BsiYI 210 CCTGAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCTTGCTTTGG
BslI 210 CCTGAAGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
273 CCTTGCTTTGG
BsmAI 827 GAGAC GTCTC(1/5)
97 GTCTC
BsmFI 675 GGGAC GGGAC(10/14)
55 GTCCC
Bsp1286I 16 GGGCTC G[AGT]GC[ACT]^C
506 GAGCAC
Bsp1407I 854 TGTACA T^GTACA
866 TGTACA
BsrGI 854 TGTACA T^GTACA
866 TGTACA
BstYI 282 GGATCT [GA]^GATC[CT]
905 AGATCT
EaeI 7 CGGCCG [CT]^GGCC[GA]
72 TGGCCG
HaeIII 8 GGCC GG^CC
73 GGCC
MaeI 503 CTAG C^TAG
991 CTAG
MflI 282 GGATCT [GA]^GATC[CT]
905 AGATCT
NlaIII 915 CATG CATG^
934 CATG
PvuII 842 CAGCTG CAG^CTG
953 CAGCTG
SspBI 854 TGTACA T^GTACA
866 TGTACA
SspI 133 AATATT AAT^ATT
314 AATATT
TaqI 140 TCGA T^CGA
825 TCGA
TfiI 66 GAATC G^A[AT]TC
519 GATTC
Tsp45I 85 GTCAC ^GT[GC]AC
939 GTCAC
TthHB8I 140 TCGA T^CGA
825 TCGA
XhoII 282 GGATCT [GA]^GATC[CT]
905 AGATCT
XmnI 132 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
427 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BcnI BsaJI BsrDI BssKI
BstNI Cac8I DpnII EcoRII Fnu4HI
HapII HinfI HpaII HphI ItaI
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV RsaI Sau3AI ScrFI
Tru9I TseI Tsp509I TspRI