Restriction map for
pFN21AE6739
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# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Aor51HI AscI Asp700I AspEI AviII
AvrII BamHI BbvCI BciVI BfaI
BfrI BlnI BsaBI BseAI BseRI
BsiWI BsmBI Bsp1407I BspEI BspHI
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
DraI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HindIII MaeI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NsiI NspV PacI PaeR7I PmeI
PshAI Psp1406I PvuI RcaI SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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Acc65I 2408 GGTACC G^GTACC
AccI 3535 GTAGAC GT^[AC][GT]AC
AocI 973 CCTGAGG CC^T[ACGT]AGG
Asp718I 2408 GGTACC G^GTACC
AspI 2050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 479 TGATCA T^GATCA
BglII 885 AGATCT A^GATCT
BsmI 2467 GAATGC GAATGC(1/-1)
BsrDI 1290 CATTGC GCAATG(2/0)
BstAPI 1670 GCAAGAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 973 CCTGAGG CC^T[ACGT]AGG
CpoI 25 CGGTCCG CG^G[AT]CCG
DraIII 2480 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 973 CCTGAGG CC^T[ACGT]AGG
EcoNI 380 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 479 TGATCA T^GATCA
HpaI 1177 GTTAAC GTT^AAC
KpnI 2408 GGTACC GGTAC^C
KspI 336 CCGCGG CCGC^GG
NruI 2046 TCGCGA TCG^CGA
PflFI 2050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1167 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 25 CGGTCCG CG^G[AT]CCG
SacI 3219 GAGCTC GAGCT^C
SacII 336 CCGCGG CCGC^GG
StuI 774 AGGCCT AGG^CCT
Tth111I 2050 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1167 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AcsI 1834 AAATTC [GA]^AATT[CT]
2952 GAATTT
AgeI 2411 ACCGGT A^CCGGT
2525 ACCGGT
Alw44I 195 GTGCAC G^TGCAC
2841 GTGCAC
ApaLI 195 GTGCAC G^TGCAC
2841 GTGCAC
ApoI 1834 AAATTC [GA]^AATT[CT]
2952 GAATTT
BbeI 32 GGCGCC GGCGC^C
169 GGCGCC
BglI 701 GCCGACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
758 GCCAGTATGGC
BlpI 1006 GCTGAGC GC^T[ACGT]AGC
3216 GCTGAGC
BpmI 2540 CTCCAG CTGGAG(16/14)
2821 CTCCAG
Bpu1102I 1006 GCTGAGC GC^T[ACGT]AGC
3216 GCTGAGC
BsaWI 2411 ACCGGT [AT]^CCGG[AT]
2525 ACCGGT
BsgI 1731 CTGCAC GTGCAG(16/14)
2787 CTGCAC
BspLU11I 2272 ACATGT A^CATGT
3036 ACATGT
CelII 1006 GCTGAGC GC^T[ACGT]AGC
3216 GCTGAGC
EagI 21 CGGCCG C^GGCCG
260 CGGCCG
EarI 3469 GAAGAG CTCTTC(1/4)
3544 GAAGAG
EclXI 21 CGGCCG C^GGCCG
260 CGGCCG
Eco52I 21 CGGCCG C^GGCCG
260 CGGCCG
HaeII 32 GGCGCC [GA]GCGC^[CT]
169 GGCGCC
KasI 32 GGCGCC G^GCGCC
169 GGCGCC
Ksp632I 3469 GAAGAG CTCTTC(1/4)
3544 GAAGAG
NaeI 491 GCCGGC GCC^GGC
3519 GCCGGC
NarI 32 GGCGCC GG^CGCC
169 GGCGCC
NcoI 1742 CCATGG C^CATGG
2594 CCATGG
NgoMIV 491 GCCGGC G^CCGGC
3519 GCCGGC
PinAI 2411 ACCGGT A^CCGGT
2525 ACCGGT
SfoI 32 GGCGCC GGC^GCC
169 GGCGCC
SmlI 235 CTCAAG C^T[CT][GA]AG
325 CTCAAG
XcmI 2649 CCAGTGCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2781 CCACCCCTGCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AseI
AsnI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvI
BcgI BcnI BmyI BpuAI BsaAI
BsaHI BsaI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspDI BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I ClaI
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmaI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmaCI
XmaI