Restriction map for
pFN21AE6509
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstUI BstXI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MflI MluI MroI
MseI MslI MspA1I MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp509I Tth111I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AfaI 228 GTAC GT^AC
AhdI 461 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 461 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 165 CCCGGG C^[CT]CG[GA]G
BalI 29 TGGCCA TGG^CCA
BanII 412 GAGCTC G[GA]GC[CT]^C
BsaWI 107 ACCGGA [AT]^CCGG[AT]
BseRI 490 CTCCTC GAGGAG(10/8)
BsoBI 165 CCCGGG C^[CT]CG[GA]G
BspMI 387 GCAGGT ACCTGC(4/8)
BsrDI 430 CATTGC GCAATG(2/0)
BsrFI 316 ACCGGC [GA]^CCGG[CT]
BstEII 468 GGTCACC G^GT[ACGT]ACC
BstF5I 264 CATCC GGATG(2/0)
BstPI 468 GGTCACC G^GT[ACGT]ACC
CfoI 455 GCGC GCG^C
Cfr10I 316 ACCGGC [GA]^CCGG[CT]
DraII 113 AGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 29 TGGCCA [CT]^GGCC[GA]
Eam1105I 461 GACTTCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 49 CTCTTC CTCTTC(1/4)
EcoO109I 113 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 468 GGTCACC G^GT[ACGT]ACC
EcoT14I 299 CCAAGG C^C[AT][AT]GG
FokI 264 CATCC GGATG(9/13)
HhaI 455 GCGC GCG^C
HinP1I 455 GCGC G^CGC
HinfI 95 GAATC G^A[ACGT]TC
Ksp632I 49 CTCTTC CTCTTC(1/4)
MboII 50 TCTTC GAAGA(8/7)
MluNI 29 TGGCCA TGG^CCA
MscI 29 TGGCCA TGG^CCA
NspI 41 GCATGC [GA]CATG^[CT]
PflMI 239 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 228 GTAC GT^AC
SacI 412 GAGCTC GAGCT^C
SapI 48 GCTCTTC GCTCTTC(1/4)
SmaI 165 CCCGGG CCC^GGG
SmlI 424 CTCAAG C^T[CT][GA]AG
SphI 41 GCATGC GCATG^C
StyI 299 CCAAGG C^C[AT][AT]GG
TaqI 197 TCGA T^CGA
TfiI 95 GAATC G^A[AT]TC
TthHB8I 197 TCGA T^CGA
Van91I 239 CCAACTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 165 CCCGGG C^CCGGG
XmaI 165 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 9 enzymes) #
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AvaII 69 GGTCC G^G[AT]CC
405 GGTCC
BcnI 165 CCCGG CC^[GC]GG
166 CCGGG
BglI 145 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
331 GCCACCCAGGC
BsrI 245 ACTGG ACTGG(1/-1)
484 CCAGT
DpnII 135 GATC ^GATC
510 GATC
MboI 135 GATC ^GATC
510 GATC
NciI 165 CCCGG CC^[GC]GG
166 CCGGG
NdeII 135 GATC ^GATC
510 GATC
Sau3AI 135 GATC ^GATC
510 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AspHI BbvI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I Cfr13I DdeI
EcoRII Fnu4HI HaeIII HapII HpaII
HphI ItaI MaeIII MnlI MspI
MvaI MwoI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI Tsp45I TspRI