Restriction map for
pFN21AE9411
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Alw44I
AlwI AocI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BamHI BbeI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HindIII HinfI
HpaI KasI KpnI KspI MamI
MfeI MflI MluI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmeI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI TfiI Tth111I
TthHB8I XbaI XcmI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AcsI 532 AAATTC [GA]^AATT[CT]
AflIII 58 ACACGT A^C[GA][CT]GT
Aor51HI 787 AGCGCT AGC^GCT
ApoI 532 AAATTC [GA]^AATT[CT]
AspHI 516 GTGCTC G[AT]GC[AT]^C
AvaI 1061 CCCGGG C^[CT]CG[GA]G
AviII 522 TGCGCA TGC^GCA
BalI 654 TGGCCA TGG^CCA
BanI 697 GGCACC G^G[CT][GA]CC
BbrPI 59 CACGTG CAC^GTG
BbvCI 366 CCTCAGC CCTCAGC(-5/-2)
BfaI 899 CTAG C^TAG
BglI 759 GCCCAAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 59 CACGTG [CT]AC^GT[GA]
BsgI 463 GTGCAG GTGCAG(16/14)
BsiHKAI 516 GTGCTC G[AT]GC[AT]^C
BsoBI 1061 CCCGGG C^[CT]CG[GA]G
BspMI 700 ACCTGC ACCTGC(4/8)
BsrFI 755 ACCGGC [GA]^CCGG[CT]
Cfr10I 755 ACCGGC [GA]^CCGG[CT]
DpnII 64 GATC ^GATC
DsaI 1047 CCGTGG C^C[GA][CT]GG
EarI 90 CTCTTC CTCTTC(1/4)
Eco47III 787 AGCGCT AGC^GCT
EcoNI 102 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 908 CCTTGG C^C[AT][AT]GG
FspI 522 TGCGCA TGC^GCA
HaeII 787 AGCGCT [GA]GCGC^[CT]
HgaI 154 GCGTC GACGC(5/10)
HincII 919 GTCAAC GT[CT]^[GA]AC
HindII 919 GTCAAC GT[CT]^[GA]AC
Ksp632I 90 CTCTTC CTCTTC(1/4)
MaeI 899 CTAG C^TAG
MboI 64 GATC ^GATC
MluNI 654 TGGCCA TGG^CCA
MscI 654 TGGCCA TGG^CCA
MseI 815 TTAA T^TAA
MspA1I 466 CAGCGG C[AC]G^C[GT]G
NdeII 64 GATC ^GATC
NheI 898 GCTAGC G^CTAGC
PmaCI 59 CACGTG CAC^GTG
PmlI 59 CACGTG CAC^GTG
PstI 30 CTGCAG CTGCA^G
Sau3AI 64 GATC ^GATC
SfcI 30 CTGCAG C^T[GA][CT]AG
SmaI 1061 CCCGGG CCC^GGG
SmlI 856 CTCAAG C^T[CT][GA]AG
StyI 908 CCTTGG C^C[AT][AT]GG
Tru9I 815 TTAA T^TAA
Tsp45I 35 GTGAC ^GT[GC]AC
Tsp509I 533 AATT ^AATT
XmaCI 1061 CCCGGG C^CCGGG
XmaI 1061 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 94 TCCGGA T^CCGGA
536 TCCGGA
AfaI 297 GTAC GT^AC
563 GTAC
AlwNI 24 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
381 CAGTAACTG
ApaI 443 GGGCCC GGGCC^C
679 GGGCCC
BanII 443 GGGCCC G[GA]GC[CT]^C
679 GGGCCC
BpmI 309 CTCCAG CTGGAG(16/14)
892 CTCCAG
BsaI 9 GGTCTC GGTCTC(1/5)
225 GGTCTC
BsaWI 94 TCCGGA [AT]^CCGG[AT]
536 TCCGGA
BseAI 94 TCCGGA T^CCGGA
536 TCCGGA
BspEI 94 TCCGGA T^CCGGA
536 TCCGGA
BsrDI 288 CATTGC GCAATG(2/0)
179 GCAATG
BsrI 380 CCAGT ACTGG(1/-1)
984 CCAGT
EaeI 503 TGGCCG [CT]^GGCC[GA]
654 TGGCCA
FauI 468 GCGGG CCCGC(4/6)
732 GCGGG
MaeIII 35 GTGAC ^GT[ACGT]AC
383 GTAAC
MroI 94 TCCGGA T^CCGGA
536 TCCGGA
PflMI 311 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
545 CCAGAAAATGG
PpuMI 50 GGGTCCT [GA]G^G[AT]CC[CT]
118 GGGTCCC
RsaI 297 GTAC GT^AC
563 GTAC
Van91I 311 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
545 CCAGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AvaII BbsI BbvI
BcnI BmyI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MboII MnlI MslI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SfaNI TseI TspRI