Restriction map for
pFN21AE4350
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BlnI BlpI Bpu1102I BsaAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MaeIII MfeI MluI
MluNI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuII
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp45I
Tsp509I Tth111I Van91I XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 805 CGCG CG^CG
AccIII 818 TCCGGA T^CCGGA
AcyI 106 GGCGTC G[GA]^CG[CT]C
AocI 525 CCTGAGG CC^T[ACGT]AGG
ApaI 7 GGGCCC GGGCC^C
Asp700I 846 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 366 GAGCTC G[AT]GC[AT]^C
BbsI 931 GAAGAC GAAGAC(2/6)
BglI 742 GCCATGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 386 AGATCT A^GATCT
BpuAI 931 GAAGAC GAAGAC(2/6)
BsaBI 228 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 106 GGCGTC G[GA]^CG[CT]C
BseAI 818 TCCGGA T^CCGGA
BsgI 550 GTGCAG GTGCAG(16/14)
BsiEI 879 CGATCG CG[GA][CT]^CG
BsiHKAI 366 GAGCTC G[AT]GC[AT]^C
BspEI 818 TCCGGA T^CCGGA
BspHI 362 TCATGA T^CATGA
BstUI 805 CGCG CG^CG
BstXI 785 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 525 CCTGAGG CC^T[ACGT]AGG
DrdI 451 GACGTTGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 659 CCGTGG C^C[GA][CT]GG
EaeI 1059 CGGCCA [CT]^GGCC[GA]
Eco81I 525 CCTGAGG CC^T[ACGT]AGG
HaeII 739 AGCGCC [GA]GCGC^[CT]
HgaI 107 GCGTC GACGC(5/10)
Hin1I 106 GGCGTC G[GA]^CG[CT]C
MaeII 452 ACGT A^CGT
MamI 228 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 818 TCCGGA T^CCGGA
MslI 302 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 691 CCGCTG C[AC]G^C[GT]G
MvnI 805 CGCG CG^CG
PaeR7I 136 CTCGAG C^TCGAG
PpuMI 500 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 796 GACCACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 637 CTGCAG CTGCA^G
PvuI 879 CGATCG CGAT^CG
RcaI 362 TCATGA T^CATGA
SacI 366 GAGCTC GAGCT^C
SfcI 637 CTGCAG C^T[GA][CT]AG
TspRI 707 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 1004 CCAACACCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 136 CTCGAG C^TCGAG
XmnI 846 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AlwNI 520 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
956 CAGATCCTG
BamHI 261 GGATCC G^GATCC
272 GGATCC
BanI 300 GGCACC G^G[CT][GA]CC
325 GGTGCC
BpmI 1038 CTCCAG CTGGAG(16/14)
1015 CTGGAG
BsaWI 95 TCCGGT [AT]^CCGG[AT]
818 TCCGGA
BseRI 631 GAGGAG GAGGAG(10/8)
940 GAGGAG
BsrBI 276 CCGCTC CCGCTC(-3/-3)
295 GAGCGG
CfoI 666 GCGC GCG^C
740 GCGC
DraII 500 AGGACCC [GA]G^G[ACGT]CC[CT]
808 GGGGCCT
EcoO109I 500 AGGACCC [GA]G^G[ACGT]CC[CT]
808 GGGGCCT
HhaI 666 GCGC GCG^C
740 GCGC
HinP1I 666 GCGC G^CGC
740 GCGC
SmlI 136 CTCGAG C^T[CT][GA]AG
997 CTTGAG
StuI 398 AGGCCT AGG^CCT
1064 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanII BbvI BcnI BmyI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI MboI MboII MflI
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SmaI
StyI TaqI Tru9I TseI TthHB8I
XhoII XmaCI XmaI