Restriction map for
pFN21AB7827
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BcgI
BciVI BclI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRV EcoT22I FauI
FbaI FseI FspI Hin1I HincII
HindII HpaI KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AocI 177 CCTAAGG CC^T[ACGT]AGG
AspHI 278 GAGCAC G[AT]GC[AT]^C
BbrPI 592 CACGTG CAC^GTG
BbvCI 496 CCTCAGC CCTCAGC(-5/-2)
BbvI 69 GCTGC GCAGC(8/12)
BcnI 1398 CCGGG CC^[GC]GG
BfaI 1069 CTAG C^TAG
BmyI 278 GAGCAC G[AGT]GC[ACT]^C
BsaAI 592 CACGTG [CT]AC^GT[GA]
BsiHKAI 278 GAGCAC G[AT]GC[AT]^C
BsmBI 1554 GAGACG CGTCTC(1/5)
Bsp1286I 278 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 1479 TGTACA T^GTACA
BspHI 810 TCATGA T^CATGA
BspMI 128 ACCTGC ACCTGC(4/8)
BsrGI 1479 TGTACA T^GTACA
Bsu36I 177 CCTAAGG CC^T[ACGT]AGG
Cac8I 299 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 1569 GCGC GCG^C
DsaI 920 CCATGG C^C[GA][CT]GG
Eco81I 177 CCTAAGG CC^T[ACGT]AGG
EcoRI 228 GAATTC G^AATTC
EcoT14I 920 CCATGG C^C[AT][AT]GG
Fnu4HI 69 GCTGC GC^[ACGT]GC
HaeII 1568 GGCGCT [GA]GCGC^[CT]
HapII 1398 CCGG C^CGG
HgaI 1556 GACGC GACGC(5/10)
HhaI 1569 GCGC GCG^C
HinP1I 1569 GCGC G^CGC
HindIII 1194 AAGCTT A^AGCTT
HpaII 1398 CCGG C^CGG
ItaI 69 GCTGC GC^[ACGT]GC
MaeI 1069 CTAG C^TAG
MaeII 593 ACGT A^CGT
MspI 1398 CCGG C^CGG
MwoI 63 GCTGGGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1398 CCGGG CC^[GC]GG
NcoI 920 CCATGG C^CATGG
PflMI 915 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 592 CACGTG CAC^GTG
PmlI 592 CACGTG CAC^GTG
RcaI 810 TCATGA T^CATGA
SexAI 334 ACCAGGT A^CC[AT]GGT
SfcI 1515 CTATAG C^T[GA][CT]AG
SspBI 1479 TGTACA T^GTACA
StuI 800 AGGCCT AGG^CCT
StyI 920 CCATGG C^C[AT][AT]GG
TseI 69 GCTGC G^C[AT]GC
Tsp45I 22 GTGAC ^GT[GC]AC
Van91I 915 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AflII 823 CTTAAG C^TTAAG
907 CTTAAG
AflIII 529 ACATGT A^C[GA][CT]GT
958 ACATGT
AlwI 243 GATCC GGATC(4/5)
1259 GATCC
BbsI 46 GTCTTC GAAGAC(2/6)
1245 GTCTTC
BfrI 823 CTTAAG C^TTAAG
907 CTTAAG
BpmI 112 CTGGAG CTGGAG(16/14)
680 CTGGAG
BpuAI 46 GTCTTC GAAGAC(2/6)
1245 GTCTTC
BsaI 1359 GAGACC GGTCTC(1/5)
338 GGTCTC
BsmFI 205 GGGAC GGGAC(10/14)
388 GTCCC
BspLU11I 529 ACATGT A^CATGT
958 ACATGT
BsrDI 1000 CATTGC GCAATG(2/0)
705 GCAATG
BsrI 679 ACTGG ACTGG(1/-1)
847 ACTGG
BstYI 242 AGATCC [GA]^GATC[CT]
1258 AGATCC
HaeIII 801 GGCC GG^CC
1401 GGCC
HphI 382 GGTGA GGTGA(8/7)
1270 GGTGA
MflI 242 AGATCC [GA]^GATC[CT]
1258 AGATCC
SapI 1134 GAAGAGC GCTCTTC(1/4)
568 GCTCTTC
XhoII 242 AGATCC [GA]^GATC[CT]
1258 AGATCC
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
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AcsI AfaI AluI ApoI Asp700I
AvaII BsaJI BseRI BsiYI BslI
BsmAI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EarI EcoRII FokI
HinfI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MvaI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TfiI Tru9I Tsp509I TspRI XmnI