Restriction map for
pFN21AE5649
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvaI AvrII BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaWI
BseAI BsgI BsiEI BsmI BsoBI
BspEI BspLU11I BsrBI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PstI PvuI PvuII RsrII SacII
SalI SbfI ScaI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AccII 367 CGCG CG^CG
AlwNI 715 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 188 AGCGCT AGC^GCT
ApaI 220 GGGCCC GGGCC^C
AspI 322 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 605 TGCGCA TGC^GCA
BalI 1331 TGGCCA TGG^CCA
BamHI 777 GGATCC G^GATCC
BbeI 493 GGCGCC GGCGC^C
BbrPI 1429 CACGTG CAC^GTG
BbsI 328 GTCTTC GAAGAC(2/6)
BbvCI 283 GCTGAGG CCTCAGC(-5/-2)
BfaI 616 CTAG C^TAG
BpmI 730 CTGGAG CTGGAG(16/14)
BpuAI 328 GTCTTC GAAGAC(2/6)
BsaAI 1429 CACGTG [CT]AC^GT[GA]
BsiWI 769 CGTACG C^GTACG
BsmBI 1088 GAGACG CGTCTC(1/5)
Bsp1407I 161 TGTACA T^GTACA
BspDI 502 ATCGAT AT^CGAT
BsrDI 116 CATTGC GCAATG(2/0)
BsrFI 1013 ACCGGC [GA]^CCGG[CT]
BsrGI 161 TGTACA T^GTACA
BssHII 366 GCGCGC G^CGCGC
BssSI 1069 CACGAG CACGAG(-5/-1)
BstUI 367 CGCG CG^CG
Cfr10I 1013 ACCGGC [GA]^CCGG[CT]
ClaI 502 ATCGAT AT^CGAT
EaeI 1331 TGGCCA [CT]^GGCC[GA]
Eco47III 188 AGCGCT AGC^GCT
EcoNI 1152 CCTCCCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 974 CCAAGG C^C[AT][AT]GG
FspI 605 TGCGCA TGC^GCA
HgaI 472 GACGC GACGC(5/10)
HincII 636 GTCAAC GT[CT]^[GA]AC
HindII 636 GTCAAC GT[CT]^[GA]AC
KasI 493 GGCGCC G^GCGCC
MaeI 616 CTAG C^TAG
MluNI 1331 TGGCCA TGG^CCA
MscI 1331 TGGCCA TGG^CCA
MseI 1268 TTAA T^TAA
MspA1I 294 CAGCGG C[AC]G^C[GT]G
MvnI 367 CGCG CG^CG
NarI 493 GGCGCC GG^CGCC
NspI 821 GCATGC [GA]CATG^[CT]
PflFI 322 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1429 CACGTG CAC^GTG
PmlI 1429 CACGTG CAC^GTG
PpuMI 105 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1342 GAGCTC GAGCT^C
SapI 81 GAAGAGC GCTCTTC(1/4)
SexAI 869 ACCTGGT A^CC[AT]GGT
SfoI 493 GGCGCC GGC^GCC
SmlI 1384 CTCAAG C^T[CT][GA]AG
SphI 821 GCATGC GCATG^C
SspBI 161 TGTACA T^GTACA
StyI 974 CCAAGG C^C[AT][AT]GG
TfiI 620 GAATC G^A[AT]TC
Tru9I 1268 TTAA T^TAA
Tsp509I 784 AATT ^AATT
Tth111I 322 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcyI 472 GACGCC G[GA]^CG[CT]C
493 GGCGCC
AflIII 1415 ACGTGT A^C[GA][CT]GT
1428 ACACGT
Asp700I 888 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1311 GAACTGTTTC
AspHI 757 GAGCAC G[AT]GC[AT]^C
1342 GAGCTC
BsaHI 472 GACGCC G[GA]^CG[CT]C
493 GGCGCC
BsaI 910 GAGACC GGTCTC(1/5)
1199 GAGACC
BsiHKAI 757 GAGCAC G[AT]GC[AT]^C
1342 GAGCTC
BspHI 173 TCATGA T^CATGA
950 TCATGA
BspMI 137 ACCTGC ACCTGC(4/8)
686 ACCTGC
BstYI 777 GGATCC [GA]^GATC[CT]
1073 AGATCC
DsaI 644 CCACGG C^C[GA][CT]GG
790 CCACGG
Hin1I 472 GACGCC G[GA]^CG[CT]C
493 GGCGCC
MaeIII 597 GTCAC ^GT[ACGT]AC
1398 GTCAC
MflI 777 GGATCC [GA]^GATC[CT]
1073 AGATCC
PleI 805 GACTC GAGTC(4/5)
1118 GAGTC
RcaI 173 TCATGA T^CATGA
950 TCATGA
StuI 707 AGGCCT AGG^CCT
1326 AGGCCT
Tsp45I 597 GTCAC ^GT[GC]AC
1398 GTCAC
XhoII 777 GGATCC [GA]^GATC[CT]
1073 AGATCC
XmnI 888 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1311 GAACTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EarI Eco81I EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TaqI
TseI TspRI TthHB8I