##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BclI        
BcnI        BfaI        BfrI        BglII       BlnI        
BpmI        BsaBI       BsaHI       BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmBI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstYI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       HapII       Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        MaeI        MaeIII      MamI        
MboI        MfeI        MflI        MluI        MroI        
MseI        MspI        MunI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SalI        SapI        Sau3AI      SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
StuI        SwaI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AspHI          67  GAGCTC               G[AT]GC[AT]^C
AvaI         1018  CCCGAG               C^[CT]CG[GA]G
BbsI          598  GTCTTC               GAAGAC(2/6)
BglI          571  GCCGTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         598  GTCTTC               GAAGAC(2/6)
BsaAI         640  TACGTG               [CT]AC^GT[GA]
BsiHKAI        67  GAGCTC               G[AT]GC[AT]^C
BsmFI         278  GTCCC                GGGAC(10/14)
BsoBI        1018  CCCGAG               C^[CT]CG[GA]G
Bsp1407I      620  TGTACA               T^GTACA
BspMI         206  ACCTGC               ACCTGC(4/8)
BsrDI         812  GCAATG               GCAATG(2/0)
BsrGI         620  TGTACA               T^GTACA
BstAPI        321  GCAGGGCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoNI         315  CCTGCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        89  CCAAGG               C^C[AT][AT]GG
HgaI          349  GCGTC                GACGC(5/10)
KspI          224  CCGCGG               CCGC^GG
MaeII         641  ACGT                 A^CGT
NspI          672  GCATGT               [GA]CATG^[CT]
PshAI         723  GACTGTTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI           67  GAGCTC               GAGCT^C
SacII         224  CCGCGG               CCGC^GG
ScaI          833  AGTACT               AGT^ACT
SfcI          824  CTGTAG               C^T[GA][CT]AG
SmlI          301  CTTGAG               C^T[CT][GA]AG
SspBI         620  TGTACA               T^GTACA
StyI           89  CCAAGG               C^C[AT][AT]GG
TaqI          586  TCGA                 T^CGA
TthHB8I       586  TCGA                 T^CGA
XcmI          704  CCACGCTCAGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AvaII         277  GGTCC                G^G[AT]CC
              525  GGACC               
BalI          356  TGGCCA               TGG^CCA
              368  TGGCCA              
BanII          67  GAGCTC               G[GA]GC[CT]^C
              578  GGGCTC              
BsaI           31  GAGACC               GGTCTC(1/5)
              700  GAGACC              
BsmAI          31  GAGAC                GTCTC(1/5)
              700  GAGAC               
BsrBI         558  CCGCTC               CCGCTC(-3/-3)
              382  GAGCGG              
EarI          235  CTCTTC               CTCTTC(1/4)
              256  CTCTTC              
Ksp632I       235  CTCTTC               CTCTTC(1/4)
              256  CTCTTC              
MluNI         356  TGGCCA               TGG^CCA
              368  TGGCCA              
MscI          356  TGGCCA               TGG^CCA
              368  TGGCCA              
MslI          372  CATCGCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              388  CACCGCAGTG          
PvuII         409  CAGCTG               CAG^CTG
              678  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        BanI        
BbvI        BlpI        BmyI        Bpu1102I    BsaJI       
BsgI        BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DsaI        EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HinfI       
ItaI        MboII       MnlI        MspA1I      MvaI        
MvnI        MwoI        NlaIII      NlaIV       PleI        
RsaI        Sau96I      ScrFI       SfaNI       TseI        
TspRI