Restriction map for
pFN21AE5626
##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BbvCI BciVI BclI
BfrI BlnI BsaI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eam1105I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HindIII
HpaI KpnI KspI MfeI MluI
MroI MseI MunI NcoI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 12 CGCG CG^CG
AcsI 808 AAATTC [GA]^AATT[CT]
Alw44I 1078 GTGCAC G^TGCAC
ApaLI 1078 GTGCAC G^TGCAC
ApoI 808 AAATTC [GA]^AATT[CT]
AspI 1401 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 386 GGCGCC GGCGC^C
BbsI 153 GAAGAC GAAGAC(2/6)
BcgI 305 GCATCATCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 989 AGATCT A^GATCT
BlpI 1291 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1291 GCTGAGC GC^T[ACGT]AGC
BpuAI 153 GAAGAC GAAGAC(2/6)
BsaWI 896 ACCGGA [AT]^CCGG[AT]
BsiEI 1008 CGGCCG CG[GA][CT]^CG
BsmBI 1345 GAGACG CGTCTC(1/5)
BstUI 12 CGCG CG^CG
CelII 1291 GCTGAGC GC^T[ACGT]AGC
DraIII 1072 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1008 CGGCCG C^GGCCG
EclXI 1008 CGGCCG C^GGCCG
Eco52I 1008 CGGCCG C^GGCCG
EcoNI 1152 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1162 GTCAAC GT[CT]^[GA]AC
HindII 1162 GTCAAC GT[CT]^[GA]AC
KasI 386 GGCGCC G^GCGCC
MaeIII 1054 GTGAC ^GT[ACGT]AC
MvnI 12 CGCG CG^CG
NaeI 528 GCCGGC GCC^GGC
NarI 386 GGCGCC GG^CGCC
NgoMIV 528 GCCGGC G^CCGGC
PflFI 1401 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1004 GAGTC GAGTC(4/5)
PstI 586 CTGCAG CTGCA^G
SfoI 386 GGCGCC GGC^GCC
TfiI 786 GATTC G^A[AT]TC
Tsp45I 1054 GTGAC ^GT[GC]AC
Tsp509I 809 AATT ^AATT
Tth111I 1401 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 233 CCACCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AfaI 949 GTAC GT^AC
1062 GTAC
AflIII 363 ACACGT A^C[GA][CT]GT
767 ACACGT
Aor51HI 662 AGCGCT AGC^GCT
719 AGCGCT
AviII 404 TGCGCA TGC^GCA
803 TGCGCA
BamHI 198 GGATCC G^GATCC
899 GGATCC
BanI 386 GGCGCC G^G[CT][GA]CC
675 GGTGCC
BbrPI 364 CACGTG CAC^GTG
768 CACGTG
BfaI 289 CTAG C^TAG
871 CTAG
BglI 285 GCCTCTAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
511 GCCATCCTGGC
BsaAI 364 CACGTG [CT]AC^GT[GA]
768 CACGTG
BsaBI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
990 GATCTACATC
BsmAI 119 GAGAC GTCTC(1/5)
1345 GAGAC
BsmFI 1413 GTCCC GGGAC(10/14)
1429 GTCCC
BssSI 736 CACGAG CACGAG(-5/-1)
37 CTCGTG
BstXI 236 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1043 CCATCACGCTGG
EarI 1129 GAAGAG CTCTTC(1/4)
1175 GAAGAG
Eco47III 662 AGCGCT AGC^GCT
719 AGCGCT
FauI 100 CCCGC CCCGC(4/6)
533 CCCGC
FspI 404 TGCGCA TGC^GCA
803 TGCGCA
HinfI 786 GATTC G^A[ACGT]TC
1004 GAGTC
Ksp632I 1129 GAAGAG CTCTTC(1/4)
1175 GAAGAG
MaeI 289 CTAG C^TAG
871 CTAG
MamI 900 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
990 GATCTACATC
PflMI 336 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1437 CCAGGGCCTGG
PmaCI 364 CACGTG CAC^GTG
768 CACGTG
PmlI 364 CACGTG CAC^GTG
768 CACGTG
RsaI 949 GTAC GT^AC
1062 GTAC
SacI 16 GAGCTC GAGCT^C
346 GAGCTC
SexAI 701 ACCTGGT A^CC[AT]GGT
1073 ACCTGGT
SfcI 586 CTGCAG C^T[GA][CT]AG
1144 CTACAG
SmaI 926 CCCGGG CCC^GGG
976 CCCGGG
TaqI 117 TCGA T^CGA
1088 TCGA
TthHB8I 117 TCGA T^CGA
1088 TCGA
Van91I 336 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1437 CCAGGGCCTGG
XmaCI 926 CCCGGG C^CCGGG
976 CCCGGG
XmaI 926 CCCGGG C^CCGGG
976 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AcyI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MaeII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PvuII Sau3AI Sau96I ScrFI
SfaNI TseI TspRI XhoII