Restriction map for
pFN21AE4569
##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AclI AcsI AflII AgeI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI AviII BbeI BbrPI
BbvCI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaBI BseRI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KasI
MamI MfeI MseI MunI NarI
NcoI NdeI NheI NotI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 280 GACGTC GACGT^C
Acc65I 1068 GGTACC G^GTACC
AccI 1441 GTAGAC GT^[AC][GT]AC
Aor51HI 578 AGCGCT AGC^GCT
ApaI 2004 GGGCCC GGGCC^C
Asp700I 1773 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1068 GGTACC G^GTACC
AspHI 1558 GTGCTC G[AT]GC[AT]^C
AvrII 1063 CCTAGG C^CTAGG
BalI 1287 TGGCCA TGG^CCA
BlnI 1063 CCTAGG C^CTAGG
BpmI 1719 CTCCAG CTGGAG(16/14)
BsaAI 1729 TACGTG [CT]AC^GT[GA]
BsiHKAI 1558 GTGCTC G[AT]GC[AT]^C
BsrBI 219 GAGCGG CCGCTC(-3/-3)
BsrDI 2123 GCAATG GCAATG(2/0)
BssSI 613 CACGAG CACGAG(-5/-1)
BstAPI 36 GCAGGACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1547 CCAACTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 996 CGGCCG C^GGCCG
EarI 1137 GAAGAG CTCTTC(1/4)
EclXI 996 CGGCCG C^GGCCG
Eco47III 578 AGCGCT AGC^GCT
Eco52I 996 CGGCCG C^GGCCG
HincII 283 GTCAAC GT[CT]^[GA]AC
HindII 283 GTCAAC GT[CT]^[GA]AC
KpnI 1068 GGTACC GGTAC^C
Ksp632I 1137 GAAGAG CTCTTC(1/4)
KspI 245 CCGCGG CCGC^GG
MluI 110 ACGCGT A^CGCGT
MluNI 1287 TGGCCA TGG^CCA
MscI 1287 TGGCCA TGG^CCA
NaeI 679 GCCGGC GCC^GGC
NgoMIV 679 GCCGGC G^CCGGC
NruI 702 TCGCGA TCG^CGA
NspI 618 GCATGC [GA]CATG^[CT]
PflMI 390 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 245 CCGCGG CCGC^GG
SapI 1137 GAAGAGC GCTCTTC(1/4)
SmlI 1810 CTCAAG C^T[CT][GA]AG
SphI 618 GCATGC GCATG^C
StuI 1605 AGGCCT AGG^CCT
TfiI 1994 GATTC G^A[AT]TC
Van91I 390 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1721 CCAGCTTCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1773 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccIII 840 TCCGGA T^CCGGA
1461 TCCGGA
BamHI 689 GGATCC G^GATCC
1037 GGATCC
BanII 504 GGGCTC G[GA]GC[CT]^C
2004 GGGCCC
BbsI 1824 GTCTTC GAAGAC(2/6)
2071 GTCTTC
BcgI 837 CGATCCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1419 CGACTTTGATGC
BpuAI 1824 GTCTTC GAAGAC(2/6)
2071 GTCTTC
BsaI 454 GAGACC GGTCTC(1/5)
2149 GAGACC
BseAI 840 TCCGGA T^CCGGA
1461 TCCGGA
BsgI 273 CTGCAC GTGCAG(16/14)
1582 GTGCAG
BsmBI 827 GAGACG CGTCTC(1/5)
1214 GAGACG
BspEI 840 TCCGGA T^CCGGA
1461 TCCGGA
BstYI 689 GGATCC [GA]^GATC[CT]
1037 GGATCC
DsaI 245 CCGCGG C^C[GA][CT]GG
1247 CCACGG
EcoT14I 413 CCAAGG C^C[AT][AT]GG
1063 CCTAGG
MflI 689 GGATCC [GA]^GATC[CT]
1037 GGATCC
MroI 840 TCCGGA T^CCGGA
1461 TCCGGA
PpuMI 860 GGGTCCC [GA]G^G[AT]CC[CT]
1907 AGGACCC
PvuII 1846 CAGCTG CAG^CTG
2158 CAGCTG
StyI 413 CCAAGG C^C[AT][AT]GG
1063 CCTAGG
XhoII 689 GGATCC [GA]^GATC[CT]
1037 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AhdI AluI AlwI AspEI AspI
AvaI AvaII BanI BbvI BcnI
BfaI BglI BmyI BsaHI BsaJI
BsaWI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI Eam1105I
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PleI PshAI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI TaqI TseI
Tsp45I TspRI Tth111I TthHB8I XmaCI
XmaI