Restriction map for
pFN21AE2064
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# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AgeI AocI ApoI AscI AseI
AsnI BbrPI BcgI BciVI BclI
BfrI BglII BlpI Bpu1102I BsaAI
BsaBI BsgI BsiWI BsmI BspDI
BspHI BssHII Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII ClaI DraI
Eco81I EcoRI EcoT22I FbaI FseI
HindIII HpaI MamI MfeI MluI
MunI NdeI NheI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI PvuII
RcaI SalI SapI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspI SwaI Tsp509I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 1898 GGTACC G^GTACC
AccIII 147 TCCGGA T^CCGGA
AflIII 1499 ACATGT A^C[GA][CT]GT
AhdI 286 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1624 GTGCAC G^TGCAC
Aor51HI 131 AGCGCT AGC^GCT
ApaI 278 GGGCCC GGGCC^C
ApaLI 1624 GTGCAC G^TGCAC
Asp718I 1898 GGTACC G^GTACC
AspEI 286 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1463 TGCGCA TGC^GCA
AvrII 531 CCTAGG C^CTAGG
BbeI 110 GGCGCC GGCGC^C
BlnI 531 CCTAGG C^CTAGG
BsaWI 147 TCCGGA [AT]^CCGG[AT]
BseAI 147 TCCGGA T^CCGGA
Bsp1407I 1349 TGTACA T^GTACA
BspEI 147 TCCGGA T^CCGGA
BspLU11I 1499 ACATGT A^CATGT
BsrGI 1349 TGTACA T^GTACA
BssSI 264 CTCGTG CACGAG(-5/-1)
BstAPI 1200 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 831 GGTGACC G^GT[ACGT]ACC
BstPI 831 GGTGACC G^GT[ACGT]ACC
DrdI 735 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 286 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 131 AGCGCT AGC^GCT
EcoNI 230 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 831 GGTGACC G^GT[ACGT]ACC
EcoRV 1423 GATATC GAT^ATC
FspI 1463 TGCGCA TGC^GCA
HincII 732 GTTGAC GT[CT]^[GA]AC
HindII 732 GTTGAC GT[CT]^[GA]AC
KasI 110 GGCGCC G^GCGCC
KpnI 1898 GGTACC GGTAC^C
MroI 147 TCCGGA T^CCGGA
MseI 1322 TTAA T^TAA
NaeI 2092 GCCGGC GCC^GGC
NarI 110 GGCGCC GG^CGCC
NcoI 1538 CCATGG C^CATGG
NgoMIV 2092 GCCGGC G^CCGGC
NotI 59 GCGGCCGC GC^GGCCGC
PaeR7I 1225 CTCGAG C^TCGAG
PshAI 1741 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 196 GAGCTC GAGCT^C
SbfI 233 CCTGCAGG CCTGCA^GG
SfoI 110 GGCGCC GGC^GCC
Sse8387I 233 CCTGCAGG CCTGCA^GG
SspBI 1349 TGTACA T^GTACA
StuI 2051 AGGCCT AGG^CCT
Tru9I 1322 TTAA T^TAA
XhoI 1225 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Asp700I 560 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1080 GAAGACATTC
AspHI 196 GAGCTC G[AT]GC[AT]^C
1624 GTGCAC
AspI 1454 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1840 GACCTGGTC
AvaI 1225 CTCGAG C^[CT]CG[GA]G
1942 CCCGAG
BamHI 144 GGATCC G^GATCC
716 GGATCC
BbsI 973 GAAGAC GAAGAC(2/6)
1080 GAAGAC
BpuAI 973 GAAGAC GAAGAC(2/6)
1080 GAAGAC
BsiHKAI 196 GAGCTC G[AT]GC[AT]^C
1624 GTGCAC
BsmBI 622 GAGACG CGTCTC(1/5)
661 GAGACG
BsoBI 1225 CTCGAG C^[CT]CG[GA]G
1942 CCCGAG
BsrDI 1809 CATTGC GCAATG(2/0)
525 GCAATG
BsrFI 1028 ACCGGC [GA]^CCGG[CT]
2092 GCCGGC
Cfr10I 1028 ACCGGC [GA]^CCGG[CT]
2092 GCCGGC
CpoI 487 CGGACCG CG^G[AT]CCG
1682 CGGACCG
DraIII 679 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1282 CACGACGTG
EagI 60 CGGCCG C^GGCCG
190 CGGCCG
EclXI 60 CGGCCG C^GGCCG
190 CGGCCG
Eco52I 60 CGGCCG C^GGCCG
190 CGGCCG
KspI 158 CCGCGG CCGC^GG
187 CCGCGG
PflFI 1454 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1840 GACCTGGTC
RsrII 487 CGGACCG CG^G[AT]CCG
1682 CGGACCG
SacII 158 CCGCGG CCGC^GG
187 CCGCGG
SmlI 1225 CTCGAG C^T[CT][GA]AG
1531 CTCAAG
TfiI 1601 GAATC G^A[AT]TC
1964 GATTC
Tth111I 1454 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1840 GACCTGGTC
XmnI 560 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1080 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI StyI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII