Restriction map for
pFN21AE9560
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AhdI AocI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbrPI BbsI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraII DraIII
DrdI Eam1105I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I RcaI RsaI RsrII SalI
SbfI ScaI SexAI SfaNI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 73 GTCTAC GT^[AC][GT]AC
AcyI 569 GGCGCC G[GA]^CG[CT]C
Alw44I 535 GTGCAC G^TGCAC
AlwNI 936 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 419 AGCGCT AGC^GCT
ApaI 548 GGGCCC GGGCC^C
ApaLI 535 GTGCAC G^TGCAC
AvaI 927 CCCGAG C^[CT]CG[GA]G
BamHI 1005 GGATCC G^GATCC
BbeI 569 GGCGCC GGCGC^C
BbvCI 843 GCTGAGG CCTCAGC(-5/-2)
BcgI 312 GCAGGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 981 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 981 GCTGAGC GC^T[ACGT]AGC
BsaHI 569 GGCGCC G[GA]^CG[CT]C
BsaWI 94 ACCGGA [AT]^CCGG[AT]
BseRI 1140 CTCCTC GAGGAG(10/8)
BsgI 802 CTGCAC GTGCAG(16/14)
BsoBI 927 CCCGAG C^[CT]CG[GA]G
BspLU11I 476 ACATGT A^CATGT
BsrBI 84 GAGCGG CCGCTC(-3/-3)
BsrI 719 ACTGG ACTGG(1/-1)
BssHII 661 GCGCGC G^CGCGC
BstAPI 537 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1005 GGATCC [GA]^GATC[CT]
CelII 981 GCTGAGC GC^T[ACGT]AGC
EarI 1063 CTCTTC CTCTTC(1/4)
Eco47III 419 AGCGCT AGC^GCT
Hin1I 569 GGCGCC G[GA]^CG[CT]C
HinfI 584 GACTC G^A[ACGT]TC
KasI 569 GGCGCC G^GCGCC
Ksp632I 1063 CTCTTC CTCTTC(1/4)
MflI 1005 GGATCC [GA]^GATC[CT]
NarI 569 GGCGCC GG^CGCC
NotI 610 GCGGCCGC GC^GGCCGC
NruI 353 TCGCGA TCG^CGA
NspI 476 ACATGT [GA]CATG^[CT]
PflMI 935 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 584 GACTC GAGTC(4/5)
PstI 637 CTGCAG CTGCA^G
PvuI 669 CGATCG CGAT^CG
PvuII 996 CAGCTG CAG^CTG
SacI 184 GAGCTC GAGCT^C
SapI 1062 GCTCTTC GCTCTTC(1/4)
SfcI 637 CTGCAG C^T[GA][CT]AG
SfiI 1092 GGCCTCCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 569 GGCGCC GGC^GCC
Van91I 935 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1005 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 476 ACATGT A^C[GA][CT]GT
1025 ACGTGT
AvaII 258 GGTCC G^G[AT]CC
924 GGACC
BpmI 1150 CTCCAG CTGGAG(16/14)
181 CTGGAG
BsaAI 964 TACGTG [CT]AC^GT[GA]
1024 TACGTG
BsmFI 1043 GGGAC GGGAC(10/14)
1082 GGGAC
EcoT14I 579 CCTTGG C^C[AT][AT]GG
1097 CCAAGG
HphI 103 GGTGA GGTGA(8/7)
734 TCACC
KspI 138 CCGCGG CCGC^GG
391 CCGCGG
MaeII 965 ACGT A^CGT
1025 ACGT
MaeIII 173 GTGAC ^GT[ACGT]AC
473 GTCAC
MboII 884 TCTTC GAAGA(8/7)
1064 TCTTC
MslI 406 CACCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
475 CACATGTATG
SacII 138 CCGCGG CCGC^GG
391 CCGCGG
StuI 710 AGGCCT AGG^CCT
1100 AGGCCT
StyI 579 CCTTGG C^C[AT][AT]GG
1097 CCAAGG
Tsp45I 173 GTGAC ^GT[GC]AC
473 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AlwI AspHI
BalI BanI BanII BbvI BcnI
BglI BmyI BsaJI BsiEI BsiHKAI
BsiYI BslI Bsp1286I BspMI BsrFI
BssKI BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EagI EclXI Eco52I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI HinP1I HpaII
ItaI MboI MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI