Restriction map for
pFN21AE1735
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BfaI
BfrI BglII BlnI BsaAI BsaHI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BspHI BspMI BsrBI BsrDI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstXI BstZ17I Bsu36I CfoI
Cfr10I CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI MaeI
MfeI MluI MluNI MscI MseI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 69 enzymes) #
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AccII 11 CGCG CG^CG
AccIII 704 TCCGGA T^CCGGA
AcsI 199 AAATTC [GA]^AATT[CT]
AflIII 122 ACATGT A^C[GA][CT]GT
AlwI 229 GATCC GGATC(4/5)
ApoI 199 AAATTC [GA]^AATT[CT]
Asp700I 318 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 411 GGACC G^G[AT]CC
BanI 46 GGTGCC G^G[CT][GA]CC
BbsI 100 GAAGAC GAAGAC(2/6)
BbvI 132 GCAGC GCAGC(8/12)
BciVI 520 GGATAC GTATCC(6/5)
BclI 540 TGATCA T^GATCA
BglI 49 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 364 GCTAAGC GC^T[ACGT]AGC
BmyI 47 GTGCCC G[AGT]GC[ACT]^C
BpmI 313 CTGGAG CTGGAG(16/14)
Bpu1102I 364 GCTAAGC GC^T[ACGT]AGC
BpuAI 100 GAAGAC GAAGAC(2/6)
BsaBI 447 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 161 GAGACC GGTCTC(1/5)
BsaWI 704 TCCGGA [AT]^CCGG[AT]
BseAI 704 TCCGGA T^CCGGA
BsmAI 161 GAGAC GTCTC(1/5)
BsmFI 410 GGGAC GGGAC(10/14)
Bsp1286I 47 GTGCCC G[AGT]GC[ACT]^C
Bsp1407I 377 TGTACA T^GTACA
BspDI 214 ATCGAT AT^CGAT
BspEI 704 TCCGGA T^CCGGA
BspLU11I 122 ACATGT A^CATGT
BsrGI 377 TGTACA T^GTACA
BstBI 142 TTCGAA TT^CGAA
BstEII 624 GGTGACC G^GT[ACGT]ACC
BstPI 624 GGTGACC G^GT[ACGT]ACC
BstUI 11 CGCG CG^CG
BstYI 228 AGATCC [GA]^GATC[CT]
CelII 364 GCTAAGC GC^T[ACGT]AGC
ClaI 214 ATCGAT AT^CGAT
EcoO65I 624 GGTGACC G^GT[ACGT]ACC
EcoT14I 371 CCAAGG C^C[AT][AT]GG
FauI 50 CCCGC CCCGC(4/6)
FbaI 540 TGATCA T^GATCA
Fnu4HI 132 GCAGC GC^[ACGT]GC
ItaI 132 GCAGC GC^[ACGT]GC
KspI 10 CCGCGG CCGC^GG
MaeII 500 ACGT A^CGT
MamI 447 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 228 AGATCC [GA]^GATC[CT]
MroI 704 TCCGGA T^CCGGA
MvnI 11 CGCG CG^CG
NspI 122 ACATGT [GA]CATG^[CT]
NspV 142 TTCGAA TT^CGAA
PflMI 564 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 410 GGGACCC [GA]G^G[AT]CC[CT]
PstI 721 CTGCAG CTGCA^G
PvuII 133 CAGCTG CAG^CTG
SacII 10 CCGCGG CCGC^GG
SapI 187 GAAGAGC GCTCTTC(1/4)
SfcI 721 CTGCAG C^T[GA][CT]AG
SfuI 142 TTCGAA TT^CGAA
SmlI 283 CTCAAG C^T[CT][GA]AG
SspBI 377 TGTACA T^GTACA
StuI 389 AGGCCT AGG^CCT
StyI 371 CCAAGG C^C[AT][AT]GG
TseI 132 GCAGC G^C[AT]GC
TspRI 168 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 564 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 228 AGATCC [GA]^GATC[CT]
XmnI 318 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 253 GTAC GT^AC
378 GTAC
AlwNI 352 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
565 CAGACCCTG
AvaI 83 CCCGGG C^[CT]CG[GA]G
221 CCCGGG
BseRI 25 GAGGAG GAGGAG(10/8)
398 GAGGAG
BsoBI 83 CCCGGG C^[CT]CG[GA]G
221 CCCGGG
DdeI 136 CTGAG C^T[ACGT]AG
365 CTAAG
DpnII 229 GATC ^GATC
541 GATC
DsaI 10 CCGCGG C^C[GA][CT]GG
509 CCACGG
MaeIII 73 GTCAC ^GT[ACGT]AC
625 GTGAC
MboI 229 GATC ^GATC
541 GATC
MspA1I 10 CCGCGG C[AC]G^C[GT]G
133 CAGCTG
NdeII 229 GATC ^GATC
541 GATC
RsaI 253 GTAC GT^AC
378 GTAC
Sau3AI 229 GATC ^GATC
541 GATC
SfaNI 109 GATGC GCATC(5/9)
490 GATGC
SmaI 83 CCCGGG CCC^GGG
221 CCCGGG
Tsp45I 73 GTCAC ^GT[GC]AC
625 GTGAC
Tsp509I 179 AATT ^AATT
200 AATT
XmaCI 83 CCCGGG C^CCGGG
221 CCCGGG
XmaI 83 CCCGGG C^CCGGG
221 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BcnI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cac8I
Cfr13I DraII EarI EcoO109I EcoRII
FokI HaeIII HapII HinfI HpaII
HphI Ksp632I MboII MnlI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TaqI TfiI TthHB8I