Restriction map for
pFN21AE9692
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I ClaI CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV FseI
FspI HpaI KasI KpnI KspI
MaeII MamI MfeI MluI MroI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI PacI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Van91I XbaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 1636 GACGCC G[GA]^CG[CT]C
AfaI 833 GTAC GT^AC
AhdI 628 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 226 GGATC GGATC(4/5)
Asp700I 288 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 628 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1026 GAGCAC G[AT]GC[AT]^C
BclI 1769 TGATCA T^GATCA
BlpI 1492 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1492 GCTCAGC GC^T[ACGT]AGC
BsaHI 1636 GACGCC G[GA]^CG[CT]C
BsiHKAI 1026 GAGCAC G[AT]GC[AT]^C
BsmI 1502 GCATTC GAATGC(1/-1)
BsrBI 1272 GAGCGG CCGCTC(-3/-3)
BstBI 1648 TTCGAA TT^CGAA
BstYI 226 GGATCT [GA]^GATC[CT]
CelII 1492 GCTCAGC GC^T[ACGT]AGC
DrdI 594 GACGGGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 628 GACCACCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1310 ATGCAT ATGCA^T
FbaI 1769 TGATCA T^GATCA
HgaI 1636 GACGC GACGC(5/10)
Hin1I 1636 GACGCC G[GA]^CG[CT]C
HincII 248 GTCAAC GT[CT]^[GA]AC
HindII 248 GTCAAC GT[CT]^[GA]AC
HindIII 1796 AAGCTT A^AGCTT
MflI 226 GGATCT [GA]^GATC[CT]
MseI 1811 TTAA T^TAA
MslI 1720 CATGCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NsiI 1310 ATGCAT ATGCA^T
NspI 1719 ACATGC [GA]CATG^[CT]
NspV 1648 TTCGAA TT^CGAA
PshAI 1321 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1556 CTGCAG CTGCA^G
PvuII 433 CAGCTG CAG^CTG
RsaI 833 GTAC GT^AC
SfuI 1648 TTCGAA TT^CGAA
Tru9I 1811 TTAA T^TAA
Tsp509I 146 AATT ^AATT
XcmI 1514 CCACCTCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 226 GGATCT [GA]^GATC[CT]
XmnI 288 GAACTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 35 enzymes) #
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AspI 38 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
820 GACGGTGTC
BbsI 330 GAAGAC GAAGAC(2/6)
1821 GAAGAC
BpuAI 330 GAAGAC GAAGAC(2/6)
1821 GAAGAC
BsaWI 1303 TCCGGT [AT]^CCGG[AT]
1817 ACCGGA
BsgI 1158 CTGCAC GTGCAG(16/14)
538 GTGCAG
BsmBI 198 CGTCTC CGTCTC(1/5)
818 GAGACG
BspMI 1101 ACCTGC ACCTGC(4/8)
1544 ACCTGC
BsrFI 236 GCCGGC [GA]^CCGG[CT]
1763 GCCGGC
BstAPI 499 GCAGACAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
540 GCAGGCGGTGC
CfoI 413 GCGC GCG^C
1806 GCGC
Cfr10I 236 GCCGGC [GA]^CCGG[CT]
1763 GCCGGC
DpnII 227 GATC ^GATC
1770 GATC
DsaI 369 CCGTGG C^C[GA][CT]GG
473 CCACGG
EarI 349 GAAGAG CTCTTC(1/4)
1048 GAAGAG
FauI 509 CCCGC CCCGC(4/6)
1428 CCCGC
HaeII 412 AGCGCC [GA]GCGC^[CT]
1805 AGCGCC
HhaI 413 GCGC GCG^C
1806 GCGC
HinP1I 413 GCGC G^CGC
1806 GCGC
Ksp632I 349 GAAGAG CTCTTC(1/4)
1048 GAAGAG
MaeIII 337 GTGAC ^GT[ACGT]AC
686 GTGAC
MboI 227 GATC ^GATC
1770 GATC
MspA1I 433 CAGCTG C[AC]G^C[GT]G
1124 CCGCTG
NaeI 236 GCCGGC GCC^GGC
1763 GCCGGC
NdeII 227 GATC ^GATC
1770 GATC
NgoMIV 236 GCCGGC G^CCGGC
1763 GCCGGC
PaeR7I 1408 CTCGAG C^TCGAG
1883 CTCGAG
PflFI 38 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
820 GACGGTGTC
Sau3AI 227 GATC ^GATC
1770 GATC
SfcI 1244 CTACAG C^T[GA][CT]AG
1556 CTGCAG
SmaI 901 CCCGGG CCC^GGG
1486 CCCGGG
Tsp45I 337 GTGAC ^GT[GC]AC
686 GTGAC
Tth111I 38 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
820 GACGGTGTC
XhoI 1408 CTCGAG C^TCGAG
1883 CTCGAG
XmaCI 901 CCCGGG C^CCGGG
1486 CCCGGG
XmaI 901 CCCGGG C^CCGGG
1486 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwNI AocI ApaI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I Cfr13I DdeI DraII
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MboII MluNI MnlI MscI MspI
MvaI MwoI NciI NlaIII NlaIV
PleI PpuMI Sau96I ScrFI SfaNI
SmlI StyI TaqI TfiI TseI
TspRI TthHB8I