Restriction map for
pFN21AE9572
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AlwI
AlwNI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BsaBI BsaI BseRI
BsgI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstYI BstZ17I Bsu36I ClaI
CpoI DpnII DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MaeIII MamI MboI MfeI
MflI MluI MseI MunI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccII 300 CGCG CG^CG
AccIII 164 TCCGGA T^CCGGA
AfaI 150 GTAC GT^AC
Alw44I 394 GTGCAC G^TGCAC
ApaI 892 GGGCCC GGGCC^C
ApaLI 394 GTGCAC G^TGCAC
BbeI 676 GGCGCC GGCGC^C
BbsI 196 GTCTTC GAAGAC(2/6)
BcnI 1006 CCCGG CC^[GC]GG
BglI 472 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1113 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1113 GCTGAGC GC^T[ACGT]AGC
BpuAI 196 GTCTTC GAAGAC(2/6)
BsaAI 898 TACGTG [CT]AC^GT[GA]
BsaWI 164 TCCGGA [AT]^CCGG[AT]
BseAI 164 TCCGGA T^CCGGA
BsmAI 13 GTCTC GTCTC(1/5)
BsmBI 12 CGTCTC CGTCTC(1/5)
BspEI 164 TCCGGA T^CCGGA
BsrDI 134 GCAATG GCAATG(2/0)
BsrFI 454 GCCGGC [GA]^CCGG[CT]
BstAPI 836 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 300 CGCG CG^CG
CelII 1113 GCTGAGC GC^T[ACGT]AGC
Cfr10I 454 GCCGGC [GA]^CCGG[CT]
EcoT14I 178 CCATGG C^C[AT][AT]GG
KasI 676 GGCGCC G^GCGCC
MroI 164 TCCGGA T^CCGGA
MvnI 300 CGCG CG^CG
NaeI 454 GCCGGC GCC^GGC
NarI 676 GGCGCC GG^CGCC
NciI 1006 CCCGG CC^[GC]GG
NcoI 178 CCATGG C^CATGG
NgoMIV 454 GCCGGC G^CCGGC
PpuMI 461 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 537 CTGCAG CTGCA^G
RsaI 150 GTAC GT^AC
SexAI 908 ACCAGGT A^CC[AT]GGT
SfoI 676 GGCGCC GGC^GCC
StuI 557 AGGCCT AGG^CCT
StyI 178 CCATGG C^C[AT][AT]GG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AcyI 676 GGCGCC G[GA]^CG[CT]C
1036 GACGCC
Aor51HI 314 AGCGCT AGC^GCT
448 AGCGCT
AvaII 462 GGTCC G^G[AT]CC
585 GGACC
BanII 892 GGGCCC G[GA]GC[CT]^C
1096 GAGCCC
BsaHI 676 GGCGCC G[GA]^CG[CT]C
1036 GACGCC
BstF5I 162 CATCC GGATG(2/0)
816 GGATG
EarI 322 CTCTTC CTCTTC(1/4)
510 GAAGAG
Eco47III 314 AGCGCT AGC^GCT
448 AGCGCT
FokI 162 CATCC GGATG(9/13)
816 GGATG
Hin1I 676 GGCGCC G[GA]^CG[CT]C
1036 GACGCC
Ksp632I 322 CTCTTC CTCTTC(1/4)
510 GAAGAG
PflMI 275 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
588 CCACGGGCTGG
Van91I 275 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
588 CCACGGGCTGG
XcmI 131 CCAGCAATGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
872 CCACAGCAGCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AluI AspHI AvaI BalI
BanI BbvI BmyI BpmI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI MaeII MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NlaIII NlaIV PvuII Sau96I
ScrFI SfaNI SfcI TseI TspRI