Restriction map for
pFN21AE2375
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbvCI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BsgI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MaeI MfeI MluI
MroI MunI NheI NotI NruI
PacI PflFI PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfcI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tth111I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 2040 GTAC GT^AC
AocI 106 CCTAAGG CC^T[ACGT]AGG
AscI 67 GGCGCGCC GG^CGCGCC
AspHI 304 GAGCTC G[AT]GC[AT]^C
BbrPI 2032 CACGTG CAC^GTG
BcgI 1466 GCATGGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 545 GTATCC GTATCC(6/5)
BsiEI 1503 CGACCG CG[GA][CT]^CG
BsiHKAI 304 GAGCTC G[AT]GC[AT]^C
BssHII 68 GCGCGC G^CGCGC
BstBI 2171 TTCGAA TT^CGAA
Bsu36I 106 CCTAAGG CC^T[ACGT]AGG
Eco81I 106 CCTAAGG CC^T[ACGT]AGG
EcoRI 352 GAATTC G^AATTC
EcoT22I 584 ATGCAT ATGCA^T
HindIII 1896 AAGCTT A^AGCTT
HinfI 2098 GAGTC G^A[ACGT]TC
NdeI 188 CATATG CA^TATG
NsiI 584 ATGCAT ATGCA^T
NspI 453 ACATGC [GA]CATG^[CT]
NspV 2171 TTCGAA TT^CGAA
PleI 2098 GAGTC GAGTC(4/5)
PmaCI 2032 CACGTG CAC^GTG
PmlI 2032 CACGTG CAC^GTG
PvuII 799 CAGCTG CAG^CTG
RsaI 2040 GTAC GT^AC
SacI 304 GAGCTC GAGCT^C
SfuI 2171 TTCGAA TT^CGAA
StuI 1952 AGGCCT AGG^CCT
Tsp45I 255 GTCAC ^GT[GC]AC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 352 GAATTC [GA]^AATT[CT]
2014 AAATTC
AcyI 1675 GGCGCC G[GA]^CG[CT]C
1714 GGCGCC
ApoI 352 GAATTC [GA]^AATT[CT]
2014 AAATTC
BbeI 1675 GGCGCC GGCGC^C
1714 GGCGCC
BbsI 416 GAAGAC GAAGAC(2/6)
599 GAAGAC
BbvI 382 GCTGC GCAGC(8/12)
801 GCTGC
BglI 1373 GCCCGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1945 GCCAGGAAGGC
BpuAI 416 GAAGAC GAAGAC(2/6)
599 GAAGAC
BsaAI 298 TACGTG [CT]AC^GT[GA]
2032 CACGTG
BsaHI 1675 GGCGCC G[GA]^CG[CT]C
1714 GGCGCC
BsrDI 1155 CATTGC GCAATG(2/0)
466 GCAATG
BsrI 490 ACTGG ACTGG(1/-1)
542 CCAGT
BstYI 438 AGATCC [GA]^GATC[CT]
1090 GGATCT
Hin1I 1675 GGCGCC G[GA]^CG[CT]C
1714 GGCGCC
KasI 1675 GGCGCC G^GCGCC
1714 GGCGCC
MflI 438 AGATCC [GA]^GATC[CT]
1090 GGATCT
MspA1I 799 CAGCTG C[AC]G^C[GT]G
1375 CCGCTG
NaeI 1240 GCCGGC GCC^GGC
1462 GCCGGC
NarI 1675 GGCGCC GG^CGCC
1714 GGCGCC
NgoMIV 1240 GCCGGC G^CCGGC
1462 GCCGGC
PaeR7I 1726 CTCGAG C^TCGAG
1765 CTCGAG
PflMI 1272 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1419 CCAGACCATGG
PpuMI 987 AGGTCCC [GA]G^G[AT]CC[CT]
1212 GGGTCCT
SexAI 266 ACCTGGT A^CC[AT]GGT
510 ACCAGGT
SfiI 1372 GGCCCGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1944 GGCCAGGAAGGCC
SfoI 1675 GGCGCC GGC^GCC
1714 GGCGCC
TseI 382 GCTGC G^C[AT]GC
801 GCTGC
Van91I 1272 CCACGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1419 CCAGACCATGG
XhoI 1726 CTCGAG C^TCGAG
1765 CTCGAG
XhoII 438 AGATCC [GA]^GATC[CT]
1090 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BalI BanI BanII
BcnI BmyI BpmI BsaBI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MamI MboI MboII
MluNI MnlI MscI MseI MslI
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI Tru9I Tsp509I TspRI TthHB8I
XcmI