Restriction map for
pFN21AE4035
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# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI KasI KpnI MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RcaI
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AlwNI 309 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 259 GGTGCC G^G[CT][GA]CC
BglI 668 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 243 CTGCAC GTGCAG(16/14)
BsmFI 231 GTCCC GGGAC(10/14)
BsmI 684 GCATTC GAATGC(1/-1)
BsrDI 534 CATTGC GCAATG(2/0)
BstNI 489 CCAGG CC^[AT]GG
BstXI 68 CCAAGGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 480 GCGC GCG^C
CpoI 93 CGGACCG CG^G[AT]CCG
DdeI 462 CTCAG C^T[ACGT]AG
EarI 553 CTCTTC CTCTTC(1/4)
EcoNI 708 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 489 CCAGG ^CC[AT]GG
FauI 482 GCGGG CCCGC(4/6)
HgaI 576 GACGC GACGC(5/10)
HhaI 480 GCGC GCG^C
HinP1I 480 GCGC G^CGC
HinfI 704 GACTC G^A[ACGT]TC
Ksp632I 553 CTCTTC CTCTTC(1/4)
KspI 391 CCGCGG CCGC^GG
MvaI 489 CCAGG CC^[AT]GG
NaeI 134 GCCGGC GCC^GGC
NgoMIV 134 GCCGGC G^CCGGC
PflMI 102 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 704 GACTC GAGTC(4/5)
PpuMI 380 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 576 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 93 CGGACCG CG^G[AT]CCG
SacII 391 CCGCGG CCGC^GG
SmaI 384 CCCGGG CCC^GGG
TaqI 357 TCGA T^CGA
TthHB8I 357 TCGA T^CGA
Van91I 102 CCAGTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 384 CCCGGG C^CCGGG
XmaI 384 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 392 CGCG CG^CG
481 CGCG
AfaI 158 GTAC GT^AC
609 GTAC
AlwI 651 GATCC GGATC(4/5)
194 GGATC
ApaI 485 GGGCCC GGGCC^C
675 GGGCCC
AvaI 384 CCCGGG C^[CT]CG[GA]G
670 CCCGAG
BanII 485 GGGCCC G[GA]GC[CT]^C
675 GGGCCC
BmyI 485 GGGCCC G[AGT]GC[ACT]^C
675 GGGCCC
BpmI 280 CTGGAG CTGGAG(16/14)
517 CTGGAG
BsaI 529 GGTCTC GGTCTC(1/5)
582 GGTCTC
BseRI 139 CTCCTC GAGGAG(10/8)
724 GAGGAG
BsoBI 384 CCCGGG C^[CT]CG[GA]G
670 CCCGAG
Bsp1286I 485 GGGCCC G[AGT]GC[ACT]^C
675 GGGCCC
BsrFI 134 GCCGGC [GA]^CCGG[CT]
247 ACCGGC
BstF5I 153 CATCC GGATG(2/0)
129 GGATG
BstUI 392 CGCG CG^CG
481 CGCG
BstYI 194 GGATCT [GA]^GATC[CT]
650 AGATCC
Cfr10I 134 GCCGGC [GA]^CCGG[CT]
247 ACCGGC
DpnII 195 GATC ^GATC
651 GATC
DraIII 170 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
366 CACAGAGTG
DsaI 391 CCGCGG C^C[GA][CT]GG
524 CCGTGG
EaeI 132 TGGCCG [CT]^GGCC[GA]
452 TGGCCG
EcoT14I 68 CCAAGG C^C[AT][AT]GG
161 CCTTGG
FokI 153 CATCC GGATG(9/13)
129 GGATG
MboI 195 GATC ^GATC
651 GATC
MflI 194 GGATCT [GA]^GATC[CT]
650 AGATCC
MvnI 392 CGCG CG^CG
481 CGCG
NdeII 195 GATC ^GATC
651 GATC
NlaIII 202 CATG CATG^
423 CATG
RsaI 158 GTAC GT^AC
609 GTAC
Sau3AI 195 GATC ^GATC
651 GATC
StyI 68 CCAAGG C^C[AT][AT]GG
161 CCTTGG
XhoII 194 GGATCT [GA]^GATC[CT]
650 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BbvI BcnI
BsaJI BsiYI BslI BsmAI BsrI
BssKI Cac8I Cfr13I DraII EcoO109I
Fnu4HI HaeIII HapII HpaII HphI
ItaI MaeIII MboII MnlI MspA1I
MspI MwoI NciI NlaIV PvuII
Sau96I ScrFI TseI Tsp45I TspRI