Restriction map for
pFN21AE6686
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AvrII
BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccII 102 CGCG CG^CG
AcyI 334 GGCGTC G[GA]^CG[CT]C
AfaI 281 GTAC GT^AC
AlwI 513 GATCC GGATC(4/5)
AviII 371 TGCGCA TGC^GCA
BalI 797 TGGCCA TGG^CCA
BbrPI 444 CACGTG CAC^GTG
BbsI 831 GAAGAC GAAGAC(2/6)
BcnI 519 CCCGG CC^[GC]GG
BglI 395 GCCTAGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 420 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 420 GCTGAGC GC^T[ACGT]AGC
BpuAI 831 GAAGAC GAAGAC(2/6)
BsaAI 444 CACGTG [CT]AC^GT[GA]
BsaHI 334 GGCGTC G[GA]^CG[CT]C
BsaI 659 GAGACC GGTCTC(1/5)
BsmAI 659 GAGAC GTCTC(1/5)
BspMI 21 GCAGGT ACCTGC(4/8)
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrDI 294 GCAATG GCAATG(2/0)
BsrFI 331 ACCGGC [GA]^CCGG[CT]
BssSI 156 CTCGTG CACGAG(-5/-1)
BstAPI 697 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 726 GGTCACC G^GT[ACGT]ACC
BstPI 726 GGTCACC G^GT[ACGT]ACC
BstUI 102 CGCG CG^CG
BstYI 512 AGATCC [GA]^GATC[CT]
CelII 420 GCTGAGC GC^T[ACGT]AGC
Cfr10I 331 ACCGGC [GA]^CCGG[CT]
DpnII 513 GATC ^GATC
EcoNI 572 CCTCTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 726 GGTCACC G^GT[ACGT]ACC
FauI 108 CCCGC CCCGC(4/6)
FspI 371 TGCGCA TGC^GCA
HgaI 335 GCGTC GACGC(5/10)
Hin1I 334 GGCGTC G[GA]^CG[CT]C
MaeII 445 ACGT A^CGT
MboI 513 GATC ^GATC
MboII 831 GAAGA GAAGA(8/7)
MflI 512 AGATCC [GA]^GATC[CT]
MluNI 797 TGGCCA TGG^CCA
MscI 797 TGGCCA TGG^CCA
MspA1I 698 CAGCTG C[AC]G^C[GT]G
MvnI 102 CGCG CG^CG
NciI 519 CCCGG CC^[GC]GG
NdeII 513 GATC ^GATC
PmaCI 444 CACGTG CAC^GTG
PmlI 444 CACGTG CAC^GTG
PvuII 698 CAGCTG CAG^CTG
RsaI 281 GTAC GT^AC
Sau3AI 513 GATC ^GATC
SfaNI 529 GATGC GCATC(5/9)
SgrAI 330 CACCGGCG C[GA]^CCGG[CT]G
StuI 87 AGGCCT AGG^CCT
XhoII 512 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 339 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
698 CAGCTGCTG
ApaI 544 GGGCCC GGGCC^C
598 GGGCCC
BfaI 85 CTAG C^TAG
397 CTAG
BsgI 327 CTGCAC GTGCAG(16/14)
264 GTGCAG
DsaI 539 CCATGG C^C[GA][CT]GG
593 CCATGG
EaeI 459 CGGCCA [CT]^GGCC[GA]
797 TGGCCA
EcoT14I 539 CCATGG C^C[AT][AT]GG
593 CCATGG
HinfI 478 GACTC G^A[ACGT]TC
808 GAGTC
MaeI 85 CTAG C^TAG
397 CTAG
NcoI 539 CCATGG C^CATGG
593 CCATGG
PleI 478 GACTC GAGTC(4/5)
808 GAGTC
PstI 237 CTGCAG CTGCA^G
378 CTGCAG
SacI 406 GAGCTC GAGCT^C
490 GAGCTC
SfcI 237 CTGCAG C^T[GA][CT]AG
378 CTGCAG
StyI 539 CCATGG C^C[AT][AT]GG
593 CCATGG
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AspHI BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MnlI MslI
MspI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI TseI Tsp45I TspRI