Restriction map for
pFN21AE1910
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# NO CUT ENZYMES ( 103 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BbeI BbrPI BbvCI
BciVI BclI BfaI BfrI BlnI
BsaAI BsaBI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoRI EcoRV EcoT22I
FbaI HaeII HindIII HpaI KasI
KpnI Ksp632I MaeI MamI MfeI
MluI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI TfiI
Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 1279 GACGCC G[GA]^CG[CT]C
AflIII 143 ACGTGT A^C[GA][CT]GT
ApaI 845 GGGCCC GGGCC^C
Asp700I 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 749 TGCGCA TGC^GCA
BalI 276 TGGCCA TGG^CCA
BamHI 1031 GGATCC G^GATCC
BbsI 1047 GAAGAC GAAGAC(2/6)
BglII 248 AGATCT A^GATCT
BlpI 990 GCTGAGC GC^T[ACGT]AGC
BpmI 130 CTGGAG CTGGAG(16/14)
Bpu1102I 990 GCTGAGC GC^T[ACGT]AGC
BpuAI 1047 GAAGAC GAAGAC(2/6)
BsaHI 1279 GACGCC G[GA]^CG[CT]C
BsaI 592 GAGACC GGTCTC(1/5)
BsmAI 592 GAGAC GTCTC(1/5)
BspHI 488 TCATGA T^CATGA
BstEII 705 GGTCACC G^GT[ACGT]ACC
BstPI 705 GGTCACC G^GT[ACGT]ACC
CelII 990 GCTGAGC GC^T[ACGT]AGC
DraIII 679 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 705 GGTCACC G^GT[ACGT]ACC
FseI 24 GGCCGGCC GGCCGG^CC
FspI 749 TGCGCA TGC^GCA
Hin1I 1279 GACGCC G[GA]^CG[CT]C
HincII 763 GTCAAC GT[CT]^[GA]AC
HindII 763 GTCAAC GT[CT]^[GA]AC
HinfI 220 GACTC G^A[ACGT]TC
KspI 1074 CCGCGG CCGC^GG
MluNI 276 TGGCCA TGG^CCA
MscI 276 TGGCCA TGG^CCA
NspI 959 GCATGT [GA]CATG^[CT]
PleI 220 GACTC GAGTC(4/5)
PpuMI 668 GGGACCT [GA]G^G[AT]CC[CT]
PstI 19 CTGCAG CTGCA^G
RcaI 488 TCATGA T^CATGA
SacI 862 GAGCTC GAGCT^C
SacII 1074 CCGCGG CCGC^GG
SbfI 18 CCTGCAGG CCTGCA^GG
SfcI 19 CTGCAG C^T[GA][CT]AG
SmaI 726 CCCGGG CCC^GGG
SmlI 643 CTCAAG C^T[CT][GA]AG
Sse8387I 18 CCTGCAGG CCTGCA^GG
StuI 1175 AGGCCT AGG^CCT
Tsp509I 172 AATT ^AATT
XmaCI 726 CCCGGG C^CCGGG
XmaI 726 CCCGGG C^CCGGG
XmnI 492 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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Alw44I 13 GTGCAC G^TGCAC
565 GTGCAC
AlwNI 985 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
1123 CAGGAGCTG
ApaLI 13 GTGCAC G^TGCAC
565 GTGCAC
AvaI 726 CCCGGG C^[CT]CG[GA]G
859 CCCGAG
BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1013 GCAGCTACTTCG
BglI 29 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
386 GCCTTCAGGGC
BsgI 787 CTGCAC GTGCAG(16/14)
997 GTGCAG
BsoBI 726 CCCGGG C^[CT]CG[GA]G
859 CCCGAG
BsrBI 185 GAGCGG CCGCTC(-3/-3)
699 GAGCGG
BsrFI 25 GCCGGC [GA]^CCGG[CT]
289 GCCGGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
289 GCCGGC
DdeI 991 CTGAG C^T[ACGT]AG
1259 CTGAG
DraII 668 GGGACCT [GA]G^G[ACGT]CC[CT]
845 GGGCCCT
EcoO109I 668 GGGACCT [GA]G^G[ACGT]CC[CT]
845 GGGCCCT
EcoT14I 108 CCATGG C^C[AT][AT]GG
215 CCATGG
HgaI 340 GACGC GACGC(5/10)
1279 GACGC
NaeI 25 GCCGGC GCC^GGC
289 GCCGGC
NcoI 108 CCATGG C^CATGG
215 CCATGG
NgoMIV 25 GCCGGC G^CCGGC
289 GCCGGC
SexAI 680 ACCTGGT A^CC[AT]GGT
1250 ACCTGGT
StyI 108 CCATGG C^C[AT][AT]GG
215 CCATGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AlwI AspHI AspI AvaII BanI
BanII BbvI BcnI BmyI BsaJI
BsaWI BseAI BseRI BsiEI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BspEI
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DpnII DsaI EaeI EagI EclXI
Eco52I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MroI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PflMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TseI Tsp45I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII