Restriction map for
pFN21AB8883
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I BsrDI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
DpnII DraI DraIII DrdI Eam1105I
EarI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MboI
MfeI MflI MluI MroI MseI
MslI MunI NcoI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
TfiI Tru9I Tsp45I Tsp509I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccII 208 CGCG CG^CG
AfaI 162 GTAC GT^AC
Alw44I 190 GTGCAC G^TGCAC
AocI 199 CCTGAGG CC^T[ACGT]AGG
Aor51HI 312 AGCGCT AGC^GCT
ApaLI 190 GTGCAC G^TGCAC
AspHI 190 GTGCAC G[AT]GC[AT]^C
AvaII 373 GGACC G^G[AT]CC
BalI 506 TGGCCA TGG^CCA
BbeI 245 GGCGCC GGCGC^C
BglI 401 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 80 ACCGGA [AT]^CCGG[AT]
BsiEI 380 CGGCCG CG[GA][CT]^CG
BsiHKAI 190 GTGCAC G[AT]GC[AT]^C
BsmAI 294 GAGAC GTCTC(1/5)
BsmFI 492 GTCCC GGGAC(10/14)
Bsp1407I 161 TGTACA T^GTACA
BspMI 213 GCAGGT ACCTGC(4/8)
BsrGI 161 TGTACA T^GTACA
BstF5I 156 GGATG GGATG(2/0)
BstUI 208 CGCG CG^CG
Bsu36I 199 CCTGAGG CC^T[ACGT]AGG
CpoI 372 CGGACCG CG^G[AT]CCG
DsaI 281 CCGTGG C^C[GA][CT]GG
EagI 380 CGGCCG C^GGCCG
EclXI 380 CGGCCG C^GGCCG
Eco47III 312 AGCGCT AGC^GCT
Eco52I 380 CGGCCG C^GGCCG
Eco81I 199 CCTGAGG CC^T[ACGT]AGG
FokI 156 GGATG GGATG(9/13)
HphI 101 TCACC GGTGA(8/7)
KasI 245 GGCGCC G^GCGCC
MboII 72 GAAGA GAAGA(8/7)
MluNI 506 TGGCCA TGG^CCA
MscI 506 TGGCCA TGG^CCA
MvnI 208 CGCG CG^CG
NarI 245 GGCGCC GG^CGCC
NspI 516 ACATGC [GA]CATG^[CT]
RsaI 162 GTAC GT^AC
RsrII 372 CGGACCG CG^G[AT]CCG
SfoI 245 GGCGCC GGC^GCC
SspBI 161 TGTACA T^GTACA
TaqI 137 TCGA T^CGA
TthHB8I 137 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 245 GGCGCC G[GA]^CG[CT]C
489 GGCGTC
AlwNI 523 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
534 CAGGGCCTG
BanI 245 GGCGCC G^G[CT][GA]CC
277 GGTGCC
BsaHI 245 GGCGCC G[GA]^CG[CT]C
489 GGCGTC
BsgI 115 CTGCAC GTGCAG(16/14)
211 GTGCAG
BsrBI 127 GAGCGG CCGCTC(-3/-3)
256 GAGCGG
BsrFI 24 GCCGGC [GA]^CCGG[CT]
378 GCCGGC
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
378 GCCGGC
DdeI 7 CTGAG C^T[ACGT]AG
200 CTGAG
EcoNI 195 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
480 CCTGGAGGAGG
EcoT14I 185 CCAAGG C^C[AT][AT]GG
509 CCAAGG
HaeII 245 GGCGCC [GA]GCGC^[CT]
312 AGCGCT
HgaI 430 GCGTC GACGC(5/10)
490 GCGTC
Hin1I 245 GGCGCC G[GA]^CG[CT]C
489 GGCGTC
HinfI 265 GAGTC G^A[ACGT]TC
296 GACTC
MspA1I 112 CAGCTG C[AC]G^C[GT]G
121 CAGCTG
NaeI 24 GCCGGC GCC^GGC
378 GCCGGC
NgoMIV 24 GCCGGC G^CCGGC
378 GCCGGC
PleI 296 GACTC GAGTC(4/5)
265 GAGTC
PvuII 112 CAGCTG CAG^CTG
121 CAGCTG
StuI 140 AGGCCT AGG^CCT
390 AGGCCT
StyI 185 CCAAGG C^C[AT][AT]GG
509 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI ApaI AvaI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiYI BslI BsoBI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvaI
MwoI NciI NlaIII NlaIV Sau96I
ScrFI SmaI TseI XmaCI XmaI