Restriction map for
pFN21AB9693
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# NO CUT ENZYMES ( 145 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AlwNI AocI ApoI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaI BseAI BsgI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DpnII DraI
DraIII DrdI Eam1105I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MboI MfeI MflI MluI MroI
MseI MslI MunI NcoI NdeI
NdeII NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tru9I Tsp509I Tth111I
TthHB8I XbaI XcmI XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 671 GTAC GT^AC
AluI 518 AGCT AG^CT
Alw44I 73 GTGCAC G^TGCAC
ApaLI 73 GTGCAC G^TGCAC
AscI 135 GGCGCGCC GG^CGCGCC
Asp700I 552 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 311 CCTCAGC CCTCAGC(-5/-2)
BsaWI 533 ACCGGA [AT]^CCGG[AT]
BsiEI 8 CGGCCG CG[GA][CT]^CG
BsrBI 507 CCGCTC CCGCTC(-3/-3)
BsrFI 115 GCCGGC [GA]^CCGG[CT]
BsrI 479 ACTGG ACTGG(1/-1)
BstXI 242 CCAGCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 115 GCCGGC [GA]^CCGG[CT]
DdeI 312 CTCAG C^T[ACGT]AG
EagI 8 CGGCCG C^GGCCG
EarI 57 CTCTTC CTCTTC(1/4)
EclXI 8 CGGCCG C^GGCCG
Eco52I 8 CGGCCG C^GGCCG
HinfI 685 GACTC G^A[ACGT]TC
Ksp632I 57 CTCTTC CTCTTC(1/4)
MaeII 41 ACGT A^CGT
MboII 58 TCTTC GAAGA(8/7)
NaeI 115 GCCGGC GCC^GGC
NgoMIV 115 GCCGGC G^CCGGC
NlaIII 48 CATG CATG^
PflMI 228 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 685 GACTC GAGTC(4/5)
PpuMI 160 GGGACCC [GA]G^G[AT]CC[CT]
PstI 696 CTGCAG CTGCA^G
RsaI 671 GTAC GT^AC
SfaNI 245 GCATC GCATC(5/9)
SfcI 696 CTGCAG C^T[GA][CT]AG
StuI 750 AGGCCT AGG^CCT
TspRI 594 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 228 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 552 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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Aor51HI 554 AGCGCT AGC^GCT
581 AGCGCT
ApaI 105 GGGCCC GGGCC^C
204 GGGCCC
BalI 50 TGGCCA TGG^CCA
605 TGGCCA
BssHII 129 GCGCGC G^CGCGC
136 GCGCGC
BstF5I 246 CATCC GGATG(2/0)
123 GGATG
Eco47III 554 AGCGCT AGC^GCT
581 AGCGCT
FokI 246 CATCC GGATG(9/13)
123 GGATG
HgaI 447 GACGC GACGC(5/10)
537 GACGC
HphI 154 TCACC GGTGA(8/7)
587 TCACC
KspI 78 CCGCGG CCGC^GG
218 CCGCGG
MaeIII 476 GTGAC ^GT[ACGT]AC
596 GTGAC
MluNI 50 TGGCCA TGG^CCA
605 TGGCCA
MscI 50 TGGCCA TGG^CCA
605 TGGCCA
SacII 78 CCGCGG CCGC^GG
218 CCGCGG
Tsp45I 476 GTGAC ^GT[GC]AC
596 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AspHI AvaI
AvaII BanI BanII BbeI BbvI
BcnI BglI BmyI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HhaI
Hin1I HinP1I HpaII ItaI KasI
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NlaIV Sau96I
ScrFI SfoI SmaI TseI XmaCI
XmaI