Restriction map for
pFN21AE6186
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# NO CUT ENZYMES ( 154 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BseAI BseRI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI DsaI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AflIII 368 ACATGT A^C[GA][CT]GT
AlwNI 232 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 27 CCCGGG C^[CT]CG[GA]G
BbrPI 217 CACGTG CAC^GTG
BpmI 499 CTGGAG CTGGAG(16/14)
BsaAI 217 CACGTG [CT]AC^GT[GA]
BsaWI 190 ACCGGA [AT]^CCGG[AT]
BsgI 334 GTGCAG GTGCAG(16/14)
BsiEI 241 CGGCCG CG[GA][CT]^CG
BsoBI 27 CCCGGG C^[CT]CG[GA]G
BspLU11I 368 ACATGT A^CATGT
BstAPI 231 GCAGGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 204 CATCC GGATG(2/0)
CfoI 13 GCGC GCG^C
EagI 241 CGGCCG C^GGCCG
EclXI 241 CGGCCG C^GGCCG
Eco52I 241 CGGCCG C^GGCCG
EcoNI 486 CCTGGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 389 CCAAGG C^C[AT][AT]GG
FokI 204 CATCC GGATG(9/13)
HgaI 360 GACGC GACGC(5/10)
HhaI 13 GCGC GCG^C
HinP1I 13 GCGC G^CGC
MaeIII 358 GTGAC ^GT[ACGT]AC
PleI 325 GACTC GAGTC(4/5)
PmaCI 217 CACGTG CAC^GTG
PmlI 217 CACGTG CAC^GTG
PpuMI 233 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 229 CTGCAG CTGCA^G
PvuII 431 CAGCTG CAG^CTG
SfaNI 203 GCATC GCATC(5/9)
SfcI 229 CTGCAG C^T[GA][CT]AG
SmaI 27 CCCGGG CCC^GGG
SphI 261 GCATGC GCATG^C
StyI 389 CCAAGG C^C[AT][AT]GG
TaqI 470 TCGA T^CGA
TfiI 467 GAATC G^A[AT]TC
Tsp45I 358 GTGAC ^GT[GC]AC
TspRI 314 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 470 TCGA T^CGA
XmaCI 27 CCCGGG C^CCGGG
XmaI 27 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
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AccII 12 CGCG CG^CG
446 CGCG
AluI 419 AGCT AG^CT
432 AGCT
AlwI 456 GATCC GGATC(4/5)
462 GATCC
AvaII 234 GGTCC G^G[AT]CC
375 GGACC
BanI 154 GGTGCC G^G[CT][GA]CC
274 GGCACC
BmyI 178 GTGCCC G[AGT]GC[ACT]^C
220 GTGCCC
BsaBI 456 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
462 GATCCGAATC
BsmFI 30 GGGAC GGGAC(10/14)
427 GGGAC
Bsp1286I 178 GTGCCC G[AGT]GC[ACT]^C
220 GTGCCC
BstUI 12 CGCG CG^CG
446 CGCG
BstYI 455 AGATCC [GA]^GATC[CT]
461 AGATCC
DpnII 456 GATC ^GATC
462 GATC
EarI 327 CTCTTC CTCTTC(1/4)
349 GAAGAG
FauI 7 CCCGC CCCGC(4/6)
447 GCGGG
HinfI 325 GACTC G^A[ACGT]TC
467 GAATC
HphI 489 GGTGA GGTGA(8/7)
119 TCACC
Ksp632I 327 CTCTTC CTCTTC(1/4)
349 GAAGAG
MamI 456 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
462 GATCCGAATC
MboI 456 GATC ^GATC
462 GATC
MflI 455 AGATCC [GA]^GATC[CT]
461 AGATCC
MspA1I 300 CAGCGG C[AC]G^C[GT]G
431 CAGCTG
MvnI 12 CGCG CG^CG
446 CGCG
NdeII 456 GATC ^GATC
462 GATC
NlaIII 262 CATG CATG^
369 CATG
NspI 261 GCATGC [GA]CATG^[CT]
368 ACATGT
Sau3AI 456 GATC ^GATC
462 GATC
SfiI 345 GGCCGAAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
474 GGCCAACGAGGCC
XcmI 389 CCAAGGCAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
476 CCAACGAGGCCCTGG
XhoII 455 AGATCC [GA]^GATC[CT]
461 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI BbvI BcnI BglI BsaJI
BsiYI BslI BspMI BssKI BstNI
Cac8I Cfr13I DraII EaeI EcoO109I
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MaeII MboII MnlI MslI
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI TseI