Restriction map for
pFN21AE3973
##################################
# NO CUT ENZYMES ( 154 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BanII
BbeI BbsI BbvCI BbvI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StyI SwaI TaqI TseI TspRI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AcsI 160 AAATTT [GA]^AATT[CT]
AflIII 927 ACATGT A^C[GA][CT]GT
Alw44I 1195 GTGCAC G^TGCAC
AlwNI 440 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1195 GTGCAC G^TGCAC
ApoI 160 AAATTT [GA]^AATT[CT]
AvaI 811 CCCGAG C^[CT]CG[GA]G
AvaII 1251 GGTCC G^G[AT]CC
BalI 469 TGGCCA TGG^CCA
BbrPI 1198 CACGTG CAC^GTG
BcnI 1101 CCCGG CC^[GC]GG
BsaAI 1198 CACGTG [CT]AC^GT[GA]
BsaBI 114 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1222 GAGACC GGTCTC(1/5)
BseRI 741 GAGGAG GAGGAG(10/8)
BsmAI 1222 GAGAC GTCTC(1/5)
BsmI 567 GCATTC GAATGC(1/-1)
BsoBI 811 CCCGAG C^[CT]CG[GA]G
BspLU11I 927 ACATGT A^CATGT
BspMI 141 ACCTGC ACCTGC(4/8)
BssSI 999 CTCGTG CACGAG(-5/-1)
BstXI 1281 CCATTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 641 GGATCT [GA]^GATC[CT]
CfoI 78 GCGC GCG^C
DraI 951 TTTAAA TTT^AAA
DraII 338 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 730 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 469 TGGCCA [CT]^GGCC[GA]
EcoNI 307 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 338 AGGCCCT [GA]G^G[ACGT]CC[CT]
Fnu4HI 274 GCCGC GC^[ACGT]GC
HaeII 77 GGCGCT [GA]GCGC^[CT]
HapII 1102 CCGG C^CGG
HhaI 78 GCGC GCG^C
HinP1I 78 GCGC G^CGC
HpaII 1102 CCGG C^CGG
ItaI 274 GCCGC GC^[ACGT]GC
MamI 114 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 641 GGATCT [GA]^GATC[CT]
MluNI 469 TGGCCA TGG^CCA
MscI 469 TGGCCA TGG^CCA
MslI 853 CACAGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 855 CAGCGG C[AC]G^C[GT]G
MspI 1102 CCGG C^CGG
NciI 1101 CCCGG CC^[GC]GG
PmaCI 1198 CACGTG CAC^GTG
PmlI 1198 CACGTG CAC^GTG
SexAI 1247 ACCTGGT A^CC[AT]GGT
StuI 315 AGGCCT AGG^CCT
TfiI 119 GAATC G^A[AT]TC
Tsp45I 626 GTGAC ^GT[GC]AC
XhoII 641 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AspHI 744 GAGCAC G[AT]GC[AT]^C
1195 GTGCAC
BmyI 744 GAGCAC G[AGT]GC[ACT]^C
1195 GTGCAC
BsaJI 657 CCCTGG C^C[ACGT][ACGT]GG
1100 CCCCGG
BsiHKAI 744 GAGCAC G[AT]GC[AT]^C
1195 GTGCAC
BsmFI 1152 GGGAC GGGAC(10/14)
1291 GGGAC
Bsp1286I 744 GAGCAC G[AGT]GC[ACT]^C
1195 GTGCAC
Cfr13I 339 GGCCC G^G[ACGT]CC
1251 GGTCC
EarI 895 CTCTTC CTCTTC(1/4)
1190 GAAGAG
Ksp632I 895 CTCTTC CTCTTC(1/4)
1190 GAAGAG
NspI 411 GCATGT [GA]CATG^[CT]
927 ACATGT
Sau96I 339 GGCCC G^G[ACGT]CC
1251 GGTCC
SspI 348 AATATT AAT^ATT
913 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AfaI AluI AlwI BfaI
BsgI BsiYI BslI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII FokI HaeIII HincII HindII
HinfI HphI MaeI MaeII MaeIII
MboI MboII MnlI MseI MvaI
MwoI NdeII NlaIII PleI RsaI
Sau3AI ScrFI SfaNI Tru9I Tsp509I