Restriction map for
pFN21AE9506
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BseRI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspHI
BspMI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
CelII CfoI Cfr10I CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HhaI HinP1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeII MamI MflI MluI
MspA1I MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccIII 911 TCCGGA T^CCGGA
AcyI 754 GGCGTC G[GA]^CG[CT]C
AflIII 641 ACATGT A^C[GA][CT]GT
AocI 142 CCTGAGG CC^T[ACGT]AGG
ApaI 773 GGGCCC GGGCC^C
Asp700I 930 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 653 TGGCCA TGG^CCA
BanII 773 GGGCCC G[GA]GC[CT]^C
BclI 869 TGATCA T^GATCA
BcnI 688 CCCGG CC^[GC]GG
BfaI 986 CTAG C^TAG
BmyI 773 GGGCCC G[AGT]GC[ACT]^C
BpmI 463 CTCCAG CTGGAG(16/14)
BsaHI 754 GGCGTC G[GA]^CG[CT]C
BsaWI 911 TCCGGA [AT]^CCGG[AT]
BseAI 911 TCCGGA T^CCGGA
BsmFI 87 GTCCC GGGAC(10/14)
BsmI 225 GCATTC GAATGC(1/-1)
Bsp1286I 773 GGGCCC G[AGT]GC[ACT]^C
BspDI 889 ATCGAT AT^CGAT
BspEI 911 TCCGGA T^CCGGA
BspLU11I 641 ACATGT A^CATGT
BsrDI 74 GCAATG GCAATG(2/0)
Bsu36I 142 CCTGAGG CC^T[ACGT]AGG
ClaI 889 ATCGAT AT^CGAT
DraII 773 GGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 18 CCGTGG C^C[GA][CT]GG
EaeI 653 TGGCCA [CT]^GGCC[GA]
Eco81I 142 CCTGAGG CC^T[ACGT]AGG
EcoO109I 773 GGGCCCC [GA]G^G[ACGT]CC[CT]
FauI 492 GCGGG CCCGC(4/6)
FbaI 869 TGATCA T^GATCA
HgaI 755 GCGTC GACGC(5/10)
Hin1I 754 GGCGTC G[GA]^CG[CT]C
MaeI 986 CTAG C^TAG
MfeI 425 CAATTG C^AATTG
MluNI 653 TGGCCA TGG^CCA
MroI 911 TCCGGA T^CCGGA
MscI 653 TGGCCA TGG^CCA
MunI 425 CAATTG C^AATTG
MwoI 616 GCTCTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 688 CCCGG CC^[GC]GG
NspI 641 ACATGT [GA]CATG^[CT]
PstI 249 CTGCAG CTGCA^G
StuI 969 AGGCCT AGG^CCT
TaqI 890 TCGA T^CGA
TthHB8I 890 TCGA T^CGA
XmnI 930 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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BanI 697 GGTGCC G^G[CT][GA]CC
712 GGTGCC
BsgI 37 CTGCAC GTGCAG(16/14)
67 CTGCAC
BsmAI 136 GTCTC GTCTC(1/5)
843 GTCTC
BstNI 656 CCAGG CC^[AT]GG
963 CCTGG
Cfr13I 773 GGGCC G^G[ACGT]CC
774 GGCCC
EcoRII 656 CCAGG ^CC[AT]GG
963 CCTGG
EcoT14I 90 CCTTGG C^C[AT][AT]GG
708 CCAAGG
HapII 689 CCGG C^CGG
912 CCGG
HpaII 689 CCGG C^CGG
912 CCGG
MslI 70 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
591 CATCACCATG
MspI 689 CCGG C^CGG
912 CCGG
MvaI 656 CCAGG CC^[AT]GG
963 CCTGG
Sau96I 773 GGGCC G^G[ACGT]CC
774 GGCCC
SfcI 249 CTGCAG C^T[GA][CT]AG
387 CTGTAG
SspI 358 AATATT AAT^ATT
795 AATATT
StyI 90 CCTTGG C^C[AT][AT]GG
708 CCAAGG
TfiI 219 GAATC G^A[AT]TC
819 GAATC
Tsp45I 154 GTGAC ^GT[GC]AC
977 GTGAC
Tsp509I 426 AATT ^AATT
880 AATT
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
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AciI AfaI AluI AlwI BbvI
BsaJI BsiYI BslI BsrI BssKI
BstF5I Cac8I DdeI DpnII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI ScrFI SfaNI Tru9I TseI
TspRI