Restriction map for
pFN21AE3714
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# NO CUT ENZYMES ( 63 enzymes) #
##################################
AccII AccIII AgeI AhdI AscI
AseI AsnI AspEI AviII AvrII
BbrPI BlnI BsaBI BsaWI BseAI
BsiEI BsiWI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstUI BstZ17I
CpoI EagI Eam1105I EclXI Eco52I
FseI FspI HindIII KspI MamI
MluI MroI MvnI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 4696 GACGTC GACGT^C
Acc65I 1678 GGTACC G^GTACC
AccI 2619 GTCTAC GT^[AC][GT]AC
AclI 3628 AACGTT AA^CGTT
AflII 5860 CTTAAG C^TTAAG
AflIII 2440 ACGTGT A^C[GA][CT]GT
Alw44I 5866 GTGCAC G^TGCAC
Aor51HI 3554 AGCGCT AGC^GCT
ApaI 16 GGGCCC GGGCC^C
ApaLI 5866 GTGCAC G^TGCAC
Asp718I 1678 GGTACC G^GTACC
BamHI 5010 GGATCC G^GATCC
BbeI 4285 GGCGCC GGCGC^C
BcgI 5083 GCAGGCAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5655 GGATAC GTATCC(6/5)
BclI 6336 TGATCA T^GATCA
BfrI 5860 CTTAAG C^TTAAG
BglII 5978 AGATCT A^GATCT
BsaAI 2622 TACGTG [CT]AC^GT[GA]
BsmBI 1537 GAGACG CGTCTC(1/5)
Bsp1407I 3966 TGTACA T^GTACA
BspDI 154 ATCGAT AT^CGAT
BsrFI 4344 GCCGGC [GA]^CCGG[CT]
BsrGI 3966 TGTACA T^GTACA
Cfr10I 4344 GCCGGC [GA]^CCGG[CT]
ClaI 154 ATCGAT AT^CGAT
Eco47III 3554 AGCGCT AGC^GCT
EcoNI 6001 CCTCTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3646 GATATC GAT^ATC
EcoT22I 2102 ATGCAT ATGCA^T
FbaI 6336 TGATCA T^GATCA
HpaI 4105 GTTAAC GTT^AAC
KasI 4285 GGCGCC G^GCGCC
KpnI 1678 GGTACC GGTAC^C
NaeI 4344 GCCGGC GCC^GGC
NarI 4285 GGCGCC GG^CGCC
NcoI 710 CCATGG C^CATGG
NgoMIV 4344 GCCGGC G^CCGGC
NsiI 2102 ATGCAT ATGCA^T
Psp1406I 3628 AACGTT AA^CGTT
ScaI 2674 AGTACT AGT^ACT
SexAI 4816 ACCAGGT A^CC[AT]GGT
SfiI 17 GGCCCCAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 4285 GGCGCC GGC^GCC
SspBI 3966 TGTACA T^GTACA
SspI 2486 AATATT AAT^ATT
XbaI 3720 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 4285 GGCGCC G[GA]^CG[CT]C
4696 GACGTC
BbvCI 4943 CCTCAGC CCTCAGC(-5/-2)
5170 GCTGAGG
BlpI 1711 GCTGAGC GC^T[ACGT]AGC
4570 GCTGAGC
Bpu1102I 1711 GCTGAGC GC^T[ACGT]AGC
4570 GCTGAGC
BsaHI 4285 GGCGCC G[GA]^CG[CT]C
4696 GACGTC
BssSI 2291 CTCGTG CACGAG(-5/-1)
3122 CTCGTG
BstEII 733 GGTGACC G^GT[ACGT]ACC
2428 GGTGACC
BstPI 733 GGTGACC G^GT[ACGT]ACC
2428 GGTGACC
BstXI 1221 CCAAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4007 CCAGACTCCTGG
CelII 1711 GCTGAGC GC^T[ACGT]AGC
4570 GCTGAGC
CfoI 3555 GCGC GCG^C
4286 GCGC
DraIII 3227 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
6196 CACTGGGTG
DrdI 2595 GACACAAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2610 GACTTCAGTGTC
EcoO65I 733 GGTGACC G^GT[ACGT]ACC
2428 GGTGACC
HaeII 3554 AGCGCT [GA]GCGC^[CT]
4285 GGCGCC
HhaI 3555 GCGC GCG^C
4286 GCGC
Hin1I 4285 GGCGCC G[GA]^CG[CT]C
4696 GACGTC
HinP1I 3555 GCGC G^CGC
4286 GCGC
HincII 4105 GTTAAC GT[CT]^[GA]AC
4663 GTTGAC
HindII 4105 GTTAAC GT[CT]^[GA]AC
4663 GTTGAC
MfeI 334 CAATTG C^AATTG
2939 CAATTG
MunI 334 CAATTG C^AATTG
2939 CAATTG
PpuMI 1299 GGGACCT [GA]G^G[AT]CC[CT]
5098 GGGACCC
PshAI 3570 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3787 GACGGTGGTC
SacI 5351 GAGCTC GAGCT^C
5905 GAGCTC
SapI 3949 GAAGAGC GCTCTTC(1/4)
5348 GAAGAGC
SmaI 462 CCCGGG CCC^GGG
3989 CCCGGG
SphI 1901 GCATGC GCATG^C
3093 GCATGC
TaqI 155 TCGA T^CGA
5282 TCGA
TthHB8I 155 TCGA T^CGA
5282 TCGA
XmaCI 462 CCCGGG C^CCGGG
3989 CCCGGG
XmaI 462 CCCGGG C^CCGGG
3989 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI Asp700I AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I Van91I XcmI XhoII
XmnI