Restriction map for
pFN21AE0599
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
Acc65I AccI AccII AclI AfaI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AvrII BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsgI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MamI MluI MvnI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TaqI TfiI TspRI TthHB8I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 526 GACGTC GACGT^C
AccIII 50 TCCGGA T^CCGGA
AcsI 11 AAATTC [GA]^AATT[CT]
Alw44I 265 GTGCAC G^TGCAC
AocI 549 CCTGAGG CC^T[ACGT]AGG
ApaLI 265 GTGCAC G^TGCAC
ApoI 11 AAATTC [GA]^AATT[CT]
AspHI 265 GTGCAC G[AT]GC[AT]^C
AspI 526 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 214 GGACC G^G[AT]CC
AviII 150 TGCGCA TGC^GCA
BamHI 252 GGATCC G^GATCC
BbeI 112 GGCGCC GGCGC^C
BcnI 40 CCCGG CC^[GC]GG
BglI 275 GCCACGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 50 TCCGGA [AT]^CCGG[AT]
BseAI 50 TCCGGA T^CCGGA
BseRI 395 CTCCTC GAGGAG(10/8)
BsiHKAI 265 GTGCAC G[AT]GC[AT]^C
BspEI 50 TCCGGA T^CCGGA
BsrDI 350 CATTGC GCAATG(2/0)
BsrI 116 CCAGT ACTGG(1/-1)
BssSI 561 CACGAG CACGAG(-5/-1)
BstF5I 401 CATCC GGATG(2/0)
BstYI 252 GGATCC [GA]^GATC[CT]
Bsu36I 549 CCTGAGG CC^T[ACGT]AGG
DraII 213 AGGACCT [GA]G^G[ACGT]CC[CT]
Eco81I 549 CCTGAGG CC^T[ACGT]AGG
EcoNI 549 CCTGAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 213 AGGACCT [GA]G^G[ACGT]CC[CT]
FauI 101 GCGGG CCCGC(4/6)
FokI 401 CATCC GGATG(9/13)
FseI 68 GGCCGGCC GGCCGG^CC
FspI 150 TGCGCA TGC^GCA
HaeII 112 GGCGCC [GA]GCGC^[CT]
HgaI 133 GCGTC GACGC(5/10)
HphI 533 TCACC GGTGA(8/7)
KasI 112 GGCGCC G^GCGCC
MaeII 527 ACGT A^CGT
MaeIII 532 GTCAC ^GT[ACGT]AC
MfeI 17 CAATTG C^AATTG
MflI 252 GGATCC [GA]^GATC[CT]
MroI 50 TCCGGA T^CCGGA
MslI 350 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 96 CAGCGG C[AC]G^C[GT]G
MunI 17 CAATTG C^AATTG
NaeI 69 GCCGGC GCC^GGC
NarI 112 GGCGCC GG^CGCC
NciI 40 CCCGG CC^[GC]GG
NgoMIV 69 GCCGGC G^CCGGC
PflFI 526 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 213 AGGACCT [GA]G^G[AT]CC[CT]
SfoI 112 GGCGCC GGC^GCC
Tsp45I 532 GTCAC ^GT[GC]AC
Tth111I 526 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 252 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 112 GGCGCC G[GA]^CG[CT]C
526 GACGTC
BalI 23 TGGCCA TGG^CCA
273 TGGCCA
BanI 112 GGCGCC G^G[CT][GA]CC
192 GGTGCC
BmyI 193 GTGCCC G[AGT]GC[ACT]^C
265 GTGCAC
BsaHI 112 GGCGCC G[GA]^CG[CT]C
526 GACGTC
BsaJI 276 CCACGG C^C[ACGT][ACGT]GG
355 CCGTGG
BsiYI 503 CCTACATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
549 CCTGAGGAAGG
BslI 503 CCTACATTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
549 CCTGAGGAAGG
Bsp1286I 193 GTGCCC G[AGT]GC[ACT]^C
265 GTGCAC
BsrFI 69 GCCGGC [GA]^CCGG[CT]
535 ACCGGC
BstNI 311 CCTGG CC^[AT]GG
543 CCTGG
Cfr10I 69 GCCGGC [GA]^CCGG[CT]
535 ACCGGC
Cfr13I 214 GGACC G^G[ACGT]CC
546 GGCCC
DpnII 253 GATC ^GATC
370 GATC
DsaI 276 CCACGG C^C[GA][CT]GG
355 CCGTGG
EarI 63 GAAGAG CTCTTC(1/4)
493 GAAGAG
EcoRII 311 CCTGG ^CC[AT]GG
543 CCTGG
Hin1I 112 GGCGCC G[GA]^CG[CT]C
526 GACGTC
Ksp632I 63 GAAGAG CTCTTC(1/4)
493 GAAGAG
MboI 253 GATC ^GATC
370 GATC
MluNI 23 TGGCCA TGG^CCA
273 TGGCCA
MscI 23 TGGCCA TGG^CCA
273 TGGCCA
MseI 248 TTAA T^TAA
407 TTAA
MvaI 311 CCTGG CC^[AT]GG
543 CCTGG
NdeII 253 GATC ^GATC
370 GATC
NlaIII 165 CATG CATG^
174 CATG
Sau3AI 253 GATC ^GATC
370 GATC
Sau96I 214 GGACC G^G[ACGT]CC
546 GGCCC
SfaNI 391 GATGC GCATC(5/9)
566 GCATC
Tru9I 248 TTAA T^TAA
407 TTAA
Tsp509I 12 AATT ^AATT
18 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI AluI AlwI AlwNI BbvI
BssKI Cac8I CfoI DdeI EaeI
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MboII MnlI MspI
MwoI NlaIV ScrFI TseI