Restriction map for
pFN21AE3216
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BcnI
BfrI BglI BlnI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StyI SwaI
Tth111I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 1191 GGTACC G^GTACC
Alw44I 1375 GTGCAC G^TGCAC
ApaLI 1375 GTGCAC G^TGCAC
Asp718I 1191 GGTACC G^GTACC
AvaI 52 CTCGAG C^[CT]CG[GA]G
BglII 168 AGATCT A^GATCT
BlpI 517 GCTCAGC GC^T[ACGT]AGC
BpmI 1424 CTCCAG CTGGAG(16/14)
Bpu1102I 517 GCTCAGC GC^T[ACGT]AGC
BsaJI 1325 CCCAGG C^C[ACGT][ACGT]GG
BsmI 1110 GAATGC GAATGC(1/-1)
BsoBI 52 CTCGAG C^[CT]CG[GA]G
BssSI 1019 CACGAG CACGAG(-5/-1)
BstAPI 1213 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 168 AGATCT [GA]^GATC[CT]
CelII 517 GCTCAGC GC^T[ACGT]AGC
Cfr13I 1408 GGCCC G^G[ACGT]CC
DraI 246 TTTAAA TTT^AAA
HincII 864 GTCAAC GT[CT]^[GA]AC
HindII 864 GTCAAC GT[CT]^[GA]AC
KpnI 1191 GGTACC GGTAC^C
MaeII 500 ACGT A^CGT
MflI 168 AGATCT [GA]^GATC[CT]
NspI 1061 GCATGT [GA]CATG^[CT]
PaeR7I 52 CTCGAG C^TCGAG
PflMI 1326 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1364 CTGCAG CTGCA^G
Sau96I 1408 GGCCC G^G[ACGT]CC
SfaNI 1210 GATGC GCATC(5/9)
SspI 1118 AATATT AAT^ATT
StuI 1431 AGGCCT AGG^CCT
Van91I 1326 CCAGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 52 CTCGAG C^TCGAG
XhoII 168 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 386 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
635 CAGAGGCTG
AocI 829 CCTGAGG CC^T[ACGT]AGG
1186 CCTGAGG
AspHI 1348 GTGCTC G[AT]GC[AT]^C
1375 GTGCAC
BanI 624 GGTGCC G^G[CT][GA]CC
1191 GGTACC
BfaI 691 CTAG C^TAG
1403 CTAG
BmyI 1348 GTGCTC G[AGT]GC[ACT]^C
1375 GTGCAC
BsiHKAI 1348 GTGCTC G[AT]GC[AT]^C
1375 GTGCAC
BsmAI 481 GTCTC GTCTC(1/5)
1279 GTCTC
Bsp1286I 1348 GTGCTC G[AGT]GC[ACT]^C
1375 GTGCAC
BspMI 1194 ACCTGC ACCTGC(4/8)
699 GCAGGT
Bsu36I 829 CCTGAGG CC^T[ACGT]AGG
1186 CCTGAGG
Cac8I 522 GCTTGC GC[ACGT]^[ACGT]GC
843 GCAGGC
Eco81I 829 CCTGAGG CC^T[ACGT]AGG
1186 CCTGAGG
MaeI 691 CTAG C^TAG
1403 CTAG
SmlI 40 CTCAAG C^T[CT][GA]AG
52 CTCGAG
TaqI 53 TCGA T^CGA
926 TCGA
TfiI 274 GAATC G^A[AT]TC
1465 GAATC
TspRI 390 CACTG CA[GC]TG[ACGT][ACGT]^
1373 CAGTG
TthHB8I 53 TCGA T^CGA
926 TCGA
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI BbvI BclI BseRI
BsgI BsiYI BslI BsmFI BsrI
BssKI BstF5I BstNI DdeI DpnII
EcoRII FbaI Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI ScrFI SfcI
Tru9I TseI Tsp45I Tsp509I