Restriction map for
pFN21AE3109
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbsI BbvCI BclI BfaI BglI
BglII BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsmAI BsmBI Bsp1407I BspEI
BspHI BspLU11I BsrGI BsrI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I DraI DraII
DrdI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
SacI SacII SalI SapI ScaI
SfiI SfoI SfuI SnaBI SpeI
SspBI SspI StyI SwaI TfiI
TspRI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 111 CGCG CG^CG
AcsI 827 AAATTT [GA]^AATT[CT]
AflII 651 CTTAAG C^TTAAG
Alw44I 616 GTGCAC G^TGCAC
ApaLI 616 GTGCAC G^TGCAC
ApoI 827 AAATTT [GA]^AATT[CT]
AspHI 616 GTGCAC G[AT]GC[AT]^C
AvaI 251 CCCGGG C^[CT]CG[GA]G
BanI 405 GGTGCC G^G[CT][GA]CC
BanII 792 GAGCCC G[GA]GC[CT]^C
BciVI 204 GGATAC GTATCC(6/5)
BfrI 651 CTTAAG C^TTAAG
BlpI 567 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 567 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 616 GTGCAC G[AT]GC[AT]^C
BsiWI 125 CGTACG C^GTACG
BsmI 693 GAATGC GAATGC(1/-1)
BsoBI 251 CCCGGG C^[CT]CG[GA]G
BspMI 402 GCAGGT ACCTGC(4/8)
BstUI 111 CGCG CG^CG
CelII 567 GCTGAGC GC^T[ACGT]AGC
CpoI 20 CGGTCCG CG^G[AT]CCG
DdeI 568 CTGAG C^T[ACGT]AG
DsaI 410 CCACGG C^C[GA][CT]GG
MaeII 71 ACGT A^CGT
MaeIII 448 GTGAC ^GT[ACGT]AC
MboII 221 GAAGA GAAGA(8/7)
MslI 609 CACGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 111 CGCG CG^CG
NspI 342 GCATGC [GA]CATG^[CT]
PvuII 819 CAGCTG CAG^CTG
RsrII 20 CGGTCCG CG^G[AT]CCG
SbfI 99 CCTGCAGG CCTGCA^GG
SexAI 620 ACCTGGT A^CC[AT]GGT
SgrAI 611 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 251 CCCGGG CCC^GGG
SphI 342 GCATGC GCATG^C
Sse8387I 99 CCTGCAGG CCTGCA^GG
StuI 425 AGGCCT AGG^CCT
Tsp45I 448 GTGAC ^GT[GC]AC
XmaCI 251 CCCGGG C^CCGGG
XmaI 251 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 126 GTAC GT^AC
230 GTAC
AvaII 21 GGTCC G^G[AT]CC
540 GGACC
BalI 509 TGGCCA TGG^CCA
680 TGGCCA
BcgI 336 CGATGAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
557 CGAGCAAGGTGC
BsmFI 120 GTCCC GGGAC(10/14)
355 GTCCC
BspDI 317 ATCGAT AT^CGAT
334 ATCGAT
BsrBI 240 CCGCTC CCGCTC(-3/-3)
637 CCGCTC
BsrDI 285 CATTGC GCAATG(2/0)
645 CATTGC
BsrFI 542 ACCGGC [GA]^CCGG[CT]
612 GCCGGT
BstXI 164 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
359 CCAAGATCCTGG
BstYI 86 AGATCC [GA]^GATC[CT]
362 AGATCC
Cfr10I 542 ACCGGC [GA]^CCGG[CT]
612 GCCGGT
ClaI 317 ATCGAT AT^CGAT
334 ATCGAT
DraIII 260 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
619 CACCTGGTG
EagI 113 CGGCCG C^GGCCG
553 CGGCCG
EclXI 113 CGGCCG C^GGCCG
553 CGGCCG
Eco52I 113 CGGCCG C^GGCCG
553 CGGCCG
HaeII 4 AGCGCC [GA]GCGC^[CT]
437 AGCGCC
HphI 186 GGTGA GGTGA(8/7)
728 TCACC
MflI 86 AGATCC [GA]^GATC[CT]
362 AGATCC
MluNI 509 TGGCCA TGG^CCA
680 TGGCCA
MscI 509 TGGCCA TGG^CCA
680 TGGCCA
MseI 326 TTAA T^TAA
652 TTAA
MspA1I 294 CCGCTG C[AC]G^C[GT]G
819 CAGCTG
NlaIII 343 CATG CATG^
532 CATG
PstI 100 CTGCAG CTGCA^G
193 CTGCAG
RsaI 126 GTAC GT^AC
230 GTAC
SfaNI 705 GATGC GCATC(5/9)
143 GCATC
SfcI 100 CTGCAG C^T[GA][CT]AG
193 CTGCAG
Tru9I 326 TTAA T^TAA
652 TTAA
Tsp509I 478 AATT ^AATT
828 AATT
XcmI 161 CCACCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
359 CCAAGATCCTGGTGG
XhoII 86 AGATCC [GA]^GATC[CT]
362 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BmyI BsaJI BsgI BsiEI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DpnII EaeI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
ItaI MboI MnlI MspI MvaI
MwoI NciI NdeII NlaIV PleI
Sau3AI Sau96I ScrFI SmlI TaqI
TseI TthHB8I