Restriction map for
pFN21AE6627
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I AspEI AvaI
AviII AvrII BalI BanII BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaWI
BseAI BsgI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HpaI MaeI MfeI MluI
MluNI MroI MscI MseI MslI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TfiI Tru9I
Tsp509I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AluI 82 AGCT AG^CT
Alw44I 94 GTGCAC G^TGCAC
ApaLI 94 GTGCAC G^TGCAC
AspI 526 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 654 GGACC G^G[AT]CC
BamHI 203 GGATCC G^GATCC
BclI 131 TGATCA T^GATCA
BsaBI 363 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 297 GGTCTC GGTCTC(1/5)
BsiEI 68 CGGCCG CG[GA][CT]^CG
BsmAI 298 GTCTC GTCTC(1/5)
BstF5I 521 GGATG GGATG(2/0)
BstYI 203 GGATCC [GA]^GATC[CT]
EaeI 68 CGGCCG [CT]^GGCC[GA]
EagI 68 CGGCCG C^GGCCG
EarI 423 GAAGAG CTCTTC(1/4)
EclXI 68 CGGCCG C^GGCCG
Eco52I 68 CGGCCG C^GGCCG
FbaI 131 TGATCA T^GATCA
FokI 521 GGATG GGATG(9/13)
HinfI 166 GACTC G^A[ACGT]TC
HphI 371 TCACC GGTGA(8/7)
Ksp632I 423 GAAGAG CTCTTC(1/4)
MaeII 308 ACGT A^CGT
MamI 363 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 203 GGATCC [GA]^GATC[CT]
NlaIII 480 CATG CATG^
PflFI 526 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 166 GACTC GAGTC(4/5)
PpuMI 653 AGGACCC [GA]G^G[AT]CC[CT]
PstI 78 CTGCAG CTGCA^G
PvuII 81 CAGCTG CAG^CTG
SapI 423 GAAGAGC GCTCTTC(1/4)
SfcI 78 CTGCAG C^T[GA][CT]AG
Tth111I 526 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 203 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 440 GGTACC G^GTACC
608 GGTACC
AfaI 441 GTAC GT^AC
609 GTAC
Asp718I 440 GGTACC G^GTACC
608 GGTACC
AspHI 94 GTGCAC G[AT]GC[AT]^C
408 GAGCAC
BbeI 4 GGCGCC GGCGC^C
621 GGCGCC
BseRI 279 CTCCTC GAGGAG(10/8)
498 CTCCTC
BsiHKAI 94 GTGCAC G[AT]GC[AT]^C
408 GAGCAC
BsmFI 353 GGGAC GGGAC(10/14)
398 GGGAC
BsrDI 333 CATTGC GCAATG(2/0)
116 GCAATG
BsrI 527 ACTGG ACTGG(1/-1)
207 CCAGT
BstNI 395 CCTGG CC^[AT]GG
558 CCAGG
DdeI 245 CTGAG C^T[ACGT]AG
406 CTGAG
DraII 240 AGGGCCT [GA]G^G[ACGT]CC[CT]
653 AGGACCC
DsaI 8 CCGCGG C^C[GA][CT]GG
612 CCGCGG
EcoO109I 240 AGGGCCT [GA]G^G[ACGT]CC[CT]
653 AGGACCC
EcoRII 395 CCTGG ^CC[AT]GG
558 CCAGG
FauI 14 CCCGC CCCGC(4/6)
99 CCCGC
HgaI 54 GACGC GACGC(5/10)
73 GACGC
KasI 4 GGCGCC G^GCGCC
621 GGCGCC
KpnI 440 GGTACC GGTAC^C
608 GGTACC
KspI 8 CCGCGG CCGC^GG
612 CCGCGG
MvaI 395 CCTGG CC^[AT]GG
558 CCAGG
NarI 4 GGCGCC GG^CGCC
621 GGCGCC
RsaI 441 GTAC GT^AC
609 GTAC
SacII 8 CCGCGG CCGC^GG
612 CCGCGG
SfaNI 363 GATGC GCATC(5/9)
578 GCATC
SfoI 4 GGCGCC GGC^GCC
621 GGCGCC
TaqI 644 TCGA T^CGA
650 TCGA
TspRI 150 CAGTG CA[GC]TG[ACGT][ACGT]^
287 CAGTG
TthHB8I 644 TCGA T^CGA
650 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AcyI AlwI BanI
BbvI BcnI BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BssKI BstUI
Cac8I CfoI Cfr13I DpnII Fnu4HI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MaeIII MboI
MboII MnlI MspA1I MspI MvnI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI TseI Tsp45I