Restriction map for
pFN21AE4126
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# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BbrPI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BseAI BsiWI BsmI
BspDI BspEI BspHI BspLU11I Bst1107I
BstBI BstZ17I ClaI DraI DrdI
Eam1105I Eco47III EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MseI MunI NdeI
NheI NruI NsiI NspI NspV
PacI PflFI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SacII SalI ScaI SexAI
SfuI SgrAI SnaBI SpeI SphI
SspI SwaI Tru9I Tth111I XbaI
XcmI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AfaI 1422 GTAC GT^AC
AgeI 939 ACCGGT A^CCGGT
Alw44I 348 GTGCAC G^TGCAC
AocI 1373 CCTCAGG CC^T[ACGT]AGG
ApaLI 348 GTGCAC G^TGCAC
AviII 485 TGCGCA TGC^GCA
BalI 611 TGGCCA TGG^CCA
BbeI 169 GGCGCC GGCGC^C
BbvCI 195 CCTCAGC CCTCAGC(-5/-2)
BcgI 981 GCAGAATTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 478 CTAG C^TAG
BsaI 537 GGTCTC GGTCTC(1/5)
BsaWI 939 ACCGGT [AT]^CCGG[AT]
BsiEI 298 CGGCCG CG[GA][CT]^CG
Bsp1407I 1421 TGTACA T^GTACA
BsrBI 376 GAGCGG CCGCTC(-3/-3)
BsrDI 1119 GCAATG GCAATG(2/0)
BsrGI 1421 TGTACA T^GTACA
BssSI 373 CACGAG CACGAG(-5/-1)
Bsu36I 1373 CCTCAGG CC^T[ACGT]AGG
CpoI 319 CGGACCG CG^G[AT]CCG
DraIII 1177 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 298 CGGCCG C^GGCCG
EclXI 298 CGGCCG C^GGCCG
Eco52I 298 CGGCCG C^GGCCG
Eco81I 1373 CCTCAGG CC^T[ACGT]AGG
EcoT14I 687 CCATGG C^C[AT][AT]GG
FspI 485 TGCGCA TGC^GCA
HinfI 745 GAATC G^A[ACGT]TC
KasI 169 GGCGCC G^GCGCC
MaeI 478 CTAG C^TAG
MaeII 188 ACGT A^CGT
MluNI 611 TGGCCA TGG^CCA
MscI 611 TGGCCA TGG^CCA
MslI 603 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 842 GCCGGC GCC^GGC
NarI 169 GGCGCC GG^CGCC
NcoI 687 CCATGG C^CATGG
NgoMIV 842 GCCGGC G^CCGGC
NotI 297 GCGGCCGC GC^GGCCGC
PflMI 810 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 939 ACCGGT A^CCGGT
RsaI 1422 GTAC GT^AC
RsrII 319 CGGACCG CG^G[AT]CCG
SapI 309 GCTCTTC GCTCTTC(1/4)
SbfI 738 CCTGCAGG CCTGCA^GG
SfoI 169 GGCGCC GGC^GCC
Sse8387I 738 CCTGCAGG CCTGCA^GG
SspBI 1421 TGTACA T^GTACA
StuI 1298 AGGCCT AGG^CCT
StyI 687 CCATGG C^C[AT][AT]GG
TfiI 745 GAATC G^A[AT]TC
Tsp509I 985 AATT ^AATT
Van91I 810 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 25 enzymes) #
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AcyI 169 GGCGCC G[GA]^CG[CT]C
1192 GGCGTC
BamHI 237 GGATCC G^GATCC
698 GGATCC
BbsI 805 GAAGAC GAAGAC(2/6)
1462 GTCTTC
BlpI 837 GCTAAGC GC^T[ACGT]AGC
1545 GCTCAGC
Bpu1102I 837 GCTAAGC GC^T[ACGT]AGC
1545 GCTCAGC
BpuAI 805 GAAGAC GAAGAC(2/6)
1462 GTCTTC
BsaHI 169 GGCGCC G[GA]^CG[CT]C
1192 GGCGTC
BsgI 339 GTGCAG GTGCAG(16/14)
1218 GTGCAG
BsmBI 1194 CGTCTC CGTCTC(1/5)
641 GAGACG
BsrFI 842 GCCGGC [GA]^CCGG[CT]
939 ACCGGT
BstAPI 341 GCAGCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
651 GCAGCGGCTGC
BstEII 438 GGTTACC G^GT[ACGT]ACC
820 GGTGACC
BstPI 438 GGTTACC G^GT[ACGT]ACC
820 GGTGACC
BstXI 38 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
893 CCAAGCCTGTGG
CelII 837 GCTAAGC GC^T[ACGT]AGC
1545 GCTCAGC
Cfr10I 842 GCCGGC [GA]^CCGG[CT]
939 ACCGGT
EcoNI 558 CCTGCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
711 CCTGGAGCAGG
EcoO65I 438 GGTTACC G^GT[ACGT]ACC
820 GGTGACC
Hin1I 169 GGCGCC G[GA]^CG[CT]C
1192 GGCGTC
PaeR7I 638 CTCGAG C^TCGAG
1474 CTCGAG
SfiI 453 GGCCACCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
770 GGCCTCTTGGGCC
SmaI 233 CCCGGG CCC^GGG
1076 CCCGGG
XhoI 638 CTCGAG C^TCGAG
1474 CTCGAG
XmaCI 233 CCCGGG C^CCGGG
1076 CCCGGG
XmaI 233 CCCGGG C^CCGGG
1076 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssHII BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PpuMI PstI PvuII SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI TaqI TseI Tsp45I TspRI
TthHB8I XhoII