Restriction map for
pFN21AE6367
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstYI BstZ17I CelII ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SnaBI Sse8387I SspBI SspI
StyI SwaI Tth111I Van91I XbaI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AclI 101 AACGTT AA^CGTT
AcyI 848 GGCGCC G[GA]^CG[CT]C
AlwNI 386 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 190 CCTGAGG CC^T[ACGT]AGG
Asp700I 382 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 145 GGACC G^G[AT]CC
BanI 848 GGCGCC G^G[CT][GA]CC
BbeI 848 GGCGCC GGCGC^C
BbvI 260 GCAGC GCAGC(8/12)
BsaHI 848 GGCGCC G[GA]^CG[CT]C
BsaJI 1019 CCGTGG C^C[ACGT][ACGT]GG
BsiYI 186 CCACCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 186 CCACCCTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 542 GAGAC GTCTC(1/5)
BsmFI 458 GGGAC GGGAC(10/14)
BsmI 159 GCATTC GAATGC(1/-1)
BsrFI 845 ACCGGC [GA]^CCGG[CT]
BstAPI 879 GCACAAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 462 CATCC GGATG(2/0)
Bsu36I 190 CCTGAGG CC^T[ACGT]AGG
CfoI 849 GCGC GCG^C
Cfr10I 845 ACCGGC [GA]^CCGG[CT]
Cfr13I 145 GGACC G^G[ACGT]CC
DdeI 191 CTGAG C^T[ACGT]AG
DsaI 1019 CCGTGG C^C[GA][CT]GG
Eco81I 190 CCTGAGG CC^T[ACGT]AGG
Fnu4HI 260 GCAGC GC^[ACGT]GC
FokI 462 CATCC GGATG(9/13)
HaeII 848 GGCGCC [GA]GCGC^[CT]
HaeIII 570 GGCC GG^CC
HapII 846 CCGG C^CGG
HhaI 849 GCGC GCG^C
Hin1I 848 GGCGCC G[GA]^CG[CT]C
HinP1I 849 GCGC G^CGC
HpaII 846 CCGG C^CGG
ItaI 260 GCAGC GC^[ACGT]GC
KasI 848 GGCGCC G^GCGCC
MaeII 102 ACGT A^CGT
MspI 846 CCGG C^CGG
MwoI 879 GCACAAGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 848 GGCGCC GG^CGCC
Psp1406I 101 AACGTT AA^CGTT
Sau96I 145 GGACC G^G[ACGT]CC
SfoI 848 GGCGCC GGC^GCC
SpeI 721 ACTAGT A^CTAGT
StuI 569 AGGCCT AGG^CCT
TfiI 952 GAATC G^A[AT]TC
TseI 260 GCAGC G^C[AT]GC
XcmI 538 CCAAGAGACTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 382 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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BpmI 977 CTCCAG CTGGAG(16/14)
968 CTGGAG
BssSI 991 CACGAG CACGAG(-5/-1)
546 CTCGTG
BstXI 166 CCATCAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
824 CCAACCACTTGG
HphI 933 GGTGA GGTGA(8/7)
179 TCACC
NspI 632 GCATGC [GA]CATG^[CT]
805 GCATGC
PleI 544 GACTC GAGTC(4/5)
419 GAGTC
SfaNI 88 GATGC GCATC(5/9)
496 GATGC
SmlI 363 CTTGAG C^T[CT][GA]AG
661 CTCAAG
SphI 632 GCATGC GCATG^C
805 GCATGC
TaqI 695 TCGA T^CGA
738 TCGA
Tsp45I 680 GTGAC ^GT[GC]AC
989 GTCAC
TthHB8I 695 TCGA T^CGA
738 TCGA
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# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
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AcsI AluI AlwI ApoI BfaI
BsrI BssKI BstNI Cac8I DpnII
EcoRII HinfI MaeI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV Sau3AI ScrFI Tru9I
Tsp509I TspRI