Restriction map for
pFN21AB9099
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MaeII
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AcsI 286 GAATTC [GA]^AATT[CT]
AluI 271 AGCT AG^CT
AlwI 518 GGATC GGATC(4/5)
AlwNI 363 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 208 GGGCCC GGGCC^C
ApoI 286 GAATTC [GA]^AATT[CT]
AspI 508 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 65 TGGCCA TGG^CCA
BglII 278 AGATCT A^GATCT
BseRI 571 CTCCTC GAGGAG(10/8)
BsmBI 456 CGTCTC CGTCTC(1/5)
BsmI 617 GAATGC GAATGC(1/-1)
BsrI 92 ACTGG ACTGG(1/-1)
BstAPI 197 GCAAGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 283 TTCGAA TT^CGAA
BstEII 81 GGTAACC G^GT[ACGT]ACC
BstPI 81 GGTAACC G^GT[ACGT]ACC
BstYI 278 AGATCT [GA]^GATC[CT]
EaeI 65 TGGCCA [CT]^GGCC[GA]
EcoNI 242 CCTACTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 81 GGTAACC G^GT[ACGT]ACC
EcoRI 286 GAATTC G^AATTC
HgaI 418 GCGTC GACGC(5/10)
HinfI 637 GAGTC G^A[ACGT]TC
MflI 278 AGATCT [GA]^GATC[CT]
MluNI 65 TGGCCA TGG^CCA
MscI 65 TGGCCA TGG^CCA
MseI 257 TTAA T^TAA
MslI 180 CATCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 283 TTCGAA TT^CGAA
PflFI 508 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 637 GAGTC GAGTC(4/5)
PshAI 413 GACCCGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 270 GAGCTC GAGCT^C
ScaI 524 AGTACT AGT^ACT
SfuI 283 TTCGAA TT^CGAA
SmaI 223 CCCGGG CCC^GGG
Tru9I 257 TTAA T^TAA
Tsp45I 31 GTGAC ^GT[GC]AC
Tsp509I 287 AATT ^AATT
Tth111I 508 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 68 CCATGACAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 278 AGATCT [GA]^GATC[CT]
XmaCI 223 CCCGGG C^CCGGG
XmaI 223 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 28 CGCG CG^CG
417 CGCG
AvaII 24 GGTCC G^G[AT]CC
539 GGTCC
BlpI 102 GCTGAGC GC^T[ACGT]AGC
652 GCTGAGC
Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC
652 GCTGAGC
BsaI 442 GGTCTC GGTCTC(1/5)
586 GGTCTC
BstUI 28 CGCG CG^CG
417 CGCG
CelII 102 GCTGAGC GC^T[ACGT]AGC
652 GCTGAGC
DpnII 279 GATC ^GATC
519 GATC
EarI 37 CTCTTC CTCTTC(1/4)
393 GAAGAG
FauI 415 CCCGC CCCGC(4/6)
206 GCGGG
Ksp632I 37 CTCTTC CTCTTC(1/4)
393 GAAGAG
MaeIII 31 GTGAC ^GT[ACGT]AC
82 GTAAC
MboI 279 GATC ^GATC
519 GATC
MvnI 28 CGCG CG^CG
417 CGCG
NcoI 185 CCATGG C^CATGG
631 CCATGG
NdeII 279 GATC ^GATC
519 GATC
NlaIV 208 GGGCCC GG[ACGT]^[ACGT]CC
626 GGAACC
Sau3AI 279 GATC ^GATC
519 GATC
TspRI 91 CACTG CA[GC]TG[ACGT][ACGT]^
501 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AspHI AvaI BanII
BbvI BcnI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MboII MnlI MspI MvaI
MwoI NciI NlaIII RsaI Sau96I
ScrFI SfaNI StyI TaqI TseI
TthHB8I