Restriction map for
pFN21AE6685
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BciVI BcnI
BfaI BfrI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KspI MaeI MamI MfeI
MluI MroI MseI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 101 GGTACC G^GTACC
AccI 394 GTCTAC GT^[AC][GT]AC
AccII 131 CGCG CG^CG
AgeI 98 ACCGGT A^CCGGT
Asp718I 101 GGTACC G^GTACC
AspHI 670 GAGCAC G[AT]GC[AT]^C
AvaII 96 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BanI 101 GGTACC G^G[CT][GA]CC
BanII 144 GAGCCC G[GA]GC[CT]^C
BcgI 82 GCAGCCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 503 TGATCA T^GATCA
BglI 4 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 67 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 67 GCTGAGC GC^T[ACGT]AGC
BsiHKAI 670 GAGCAC G[AT]GC[AT]^C
BsmAI 18 GTCTC GTCTC(1/5)
BsmFI 294 GTCCC GGGAC(10/14)
BspHI 485 TCATGA T^CATGA
BsrDI 215 GCAATG GCAATG(2/0)
BsrFI 98 ACCGGT [GA]^CCGG[CT]
BstUI 131 CGCG CG^CG
BstYI 134 GGATCT [GA]^GATC[CT]
CelII 67 GCTGAGC GC^T[ACGT]AGC
CfoI 206 GCGC GCG^C
Cfr10I 98 ACCGGT [GA]^CCGG[CT]
DraII 590 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO109I 590 AGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 503 TGATCA T^GATCA
HaeII 205 AGCGCC [GA]GCGC^[CT]
HhaI 206 GCGC GCG^C
HinP1I 206 GCGC G^CGC
KpnI 101 GGTACC GGTAC^C
MaeII 150 ACGT A^CGT
MaeIII 631 GTGAC ^GT[ACGT]AC
MflI 134 GGATCT [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 516 CATCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 131 CGCG CG^CG
NlaIV 101 GGTACC GG[ACGT]^[ACGT]CC
PinAI 98 ACCGGT A^CCGGT
RcaI 485 TCATGA T^CATGA
SfcI 662 CTGTAG C^T[GA][CT]AG
Tsp45I 631 GTGAC ^GT[GC]AC
XcmI 29 CCAGCCACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 134 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 15 enzymes) #
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AfaI 76 GTAC GT^AC
102 GTAC
AlwI 489 GATCC GGATC(4/5)
134 GGATC
BsrI 35 ACTGG ACTGG(1/-1)
73 CCAGT
DdeI 63 CTCAG C^T[ACGT]AG
68 CTGAG
EarI 511 CTCTTC CTCTTC(1/4)
289 GAAGAG
EcoT14I 327 CCAAGG C^C[AT][AT]GG
440 CCTTGG
HgaI 495 GACGC GACGC(5/10)
633 GACGC
Ksp632I 511 CTCTTC CTCTTC(1/4)
289 GAAGAG
PflMI 29 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
33 CCACTGGATGG
PleI 292 GAGTC GAGTC(4/5)
422 GAGTC
RsaI 76 GTAC GT^AC
102 GTAC
SfaNI 332 GCATC GCATC(5/9)
353 GCATC
StyI 327 CCAAGG C^C[AT][AT]GG
440 CCTTGG
TspRI 34 CACTG CA[GC]TG[ACGT][ACGT]^
190 CACTG
Van91I 29 CCAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
33 CCACTGGATGG
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AluI AlwNI BbvI BmyI
BsaJI BsaWI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII PvuII
Sau3AI Sau96I ScrFI TfiI TseI