Restriction map for
pFN21AE9282
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# NO CUT ENZYMES ( 117 enzymes) #
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AatII AccII AcyI AflII AflIII
AgeI AlwNI Aor51HI ApaI AscI
Asp700I AspI AvaII AviII AvrII
BbeI BbrPI BcnI BfrI BglI
BlnI BsaAI BsaHI BsaI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CfoI Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV EcoT22I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI Ksp632I KspI
MluI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 1945 GGTACC G^GTACC
AccI 2718 GTCTAC GT^[AC][GT]AC
AccIII 788 TCCGGA T^CCGGA
AciI 3274 CCGC CCGC(-3/-1)
AhdI 2710 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2627 GTGCAC G^TGCAC
AocI 856 CCTGAGG CC^T[ACGT]AGG
ApaLI 2627 GTGCAC G^TGCAC
Asp718I 1945 GGTACC G^GTACC
AspEI 2710 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 633 TGGCCA TGG^CCA
BamHI 3297 GGATCC G^GATCC
BanI 1945 GGTACC G^G[CT][GA]CC
BbvCI 3559 GCTGAGG CCTCAGC(-5/-2)
BciVI 2680 GTATCC GTATCC(6/5)
BclI 960 TGATCA T^GATCA
BglII 2370 AGATCT A^GATCT
BlpI 158 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 158 GCTTAGC GC^T[ACGT]AGC
BsaBI 1144 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 788 TCCGGA [AT]^CCGG[AT]
BseAI 788 TCCGGA T^CCGGA
BsmAI 3315 GTCTC GTCTC(1/5)
BspEI 788 TCCGGA T^CCGGA
BspMI 2217 GCAGGT ACCTGC(4/8)
BsrBI 3274 CCGCTC CCGCTC(-3/-3)
BsrDI 520 CATTGC GCAATG(2/0)
BstXI 2478 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 856 CCTGAGG CC^T[ACGT]AGG
CelII 158 GCTTAGC GC^T[ACGT]AGC
Eam1105I 2710 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 856 CCTGAGG CC^T[ACGT]AGG
FbaI 960 TGATCA T^GATCA
HapII 789 CCGG C^CGG
HgaI 2230 GACGC GACGC(5/10)
HincII 1276 GTCAAC GT[CT]^[GA]AC
HindII 1276 GTCAAC GT[CT]^[GA]AC
HpaII 789 CCGG C^CGG
KpnI 1945 GGTACC GGTAC^C
MamI 1144 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 633 TGGCCA TGG^CCA
MroI 788 TCCGGA T^CCGGA
MscI 633 TGGCCA TGG^CCA
MspI 789 CCGG C^CGG
PflMI 1516 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SphI 265 GCATGC GCATG^C
StuI 1043 AGGCCT AGG^CCT
SwaI 1145 ATTTAAAT ATTT^AAAT
Van91I 1516 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1116 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 18 enzymes) #
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AclI 1733 AACGTT AA^CGTT
2117 AACGTT
AseI 1336 ATTAAT AT^TAAT
1807 ATTAAT
AsnI 1336 ATTAAT AT^TAAT
1807 ATTAAT
BcgI 1194 CGAGGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1315 CGAGCTGTGTGC
BseRI 1531 GAGGAG GAGGAG(10/8)
2186 GAGGAG
BspHI 1302 TCATGA T^CATGA
3678 TCATGA
EaeI 633 TGGCCA [CT]^GGCC[GA]
2436 TGGCCG
MfeI 26 CAATTG C^AATTG
247 CAATTG
MslI 841 CATACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2503 CACAGAAATG
MspA1I 1787 CAGCTG C[AC]G^C[GT]G
1900 CAGCTG
MunI 26 CAATTG C^AATTG
247 CAATTG
NspI 265 GCATGC [GA]CATG^[CT]
3728 GCATGT
PshBI 1336 ATTAAT AT^TAAT
1807 ATTAAT
Psp1406I 1733 AACGTT AA^CGTT
2117 AACGTT
PstI 1751 CTGCAG CTGCA^G
2215 CTGCAG
PvuII 1787 CAGCTG CAG^CTG
1900 CAGCTG
RcaI 1302 TCATGA T^CATGA
3678 TCATGA
Tsp45I 69 GTCAC ^GT[GC]AC
2731 GTGAC
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# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
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AcsI AfaI AluI AlwI ApoI
AspHI AvaI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EcoO109I EcoRI EcoRII
EcoT14I Fnu4HI FokI HaeIII HindIII
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XhoII